Agarose gel electrophoresis

Electrophoresis; Separation of Proteins Purpose: To create an electrophoresis gel in order to further separate Cytochrome C, Myoglobin, Albumin and Hemoglobin(proteins) based off of their net charges. Materials: • Rabbit hemoglobin • Serum albumin(cow) • Myoglobin • Cytochrome C • Microtubes • Tape • Micropipets • Agarose gel • Buffer • Gel chamber • Test tubes • Test tube holders • Gloves • Macropipets Properties of Proteins: Protein Color Isoelectric Point Net Change at pH 8.6 Cytochrome C…

samples is Gel Electrophoresis (GE). Overview of Gel Electrophoresis: Gel electrophoresis is an effective and widely used method that separates and identifies molecules on the basis of electrical charge and size. GE is a particularly useful method for separating amino acids, which are charged, organic molecules such as DNA (deoxyribonucleic acids), RNA (ribonucleic acids), and proteins.…

and genetics. Although DNA is typically used in agarose gel electrophoresis, it was not provided for this lab, so the use of three different dyes had to make do. A null hypothesis was created indicating that dyes with a lighter molecular weight would not pass through the agarose gel easier than those with a heavier molecular weight. Where as the alternate hypothesis stated that, dyes with a lighter molecular weight would pass through the agarose gel easier than those with a heavier molecular…

damages. Students must keep hands away from face, eyes, and mouth, when being in contact with chemicals. Students will enter this lab with basic knowledge on how agarose gel electrophoresis is used to separate proteins. This lab experiment will approximately take 20 minutes to gather all the materials needed and to prepare the agarose gel for use. The run time for the experiment can range between 2 to 3 hours depending on the accuracy and pace of the students. It is important to follow…

Electrophoresis Kendra Gibson November 17, 2015 A scientist drops DNA samples in small indentations at the end of a Jell-O-like gel. He turns on an electrical current at the other side of the gel. His anticipation grows as he watches the samples slowly move from one side to the other. He pulls out a ruler and makes some measurements. He steps back in surprise, for he had just identified the most feared serial killer in the world! He did this by using Gel Electrophoresis. Electrophoresis (the…

In Procedure 6.1, two different gels were used. One gel was 2.0% agarose while the other gel was 0.8% agarose. The reason we poured two gels was because each gel had a different purpose. The 2.0% agarose (thicker gel) was used to determine amplicons while the 0.8% agarose (thinner gel) was used for restriction fragments. One gel was ‘thicker’ than the other gel to distinguish smaller segments than the thinner gel. By having a thicker gel, smaller segments can move better and not be cleared out…

Figure legend: Figure 3 shows the graphed bands given from the picture taken from the blue box after electrophoresis. The graph provided an equation that then allows to solve for the other established base pairs. Figure 4 Figure legend. Figure 4 shows the restriction map of the plasmid indicating the restriction sites. Discussion The gel was analyzed through the method of agarose gel electrophoresis. Once the process was done, a picture was taken to show the distances between the bands as…

and this experiment illustrated the versatility of these molecular biology techniques of PCR and gel electrophoresis to serve as methods identifying individuals. Both of the hypotheses made in this experiment that (1) there were genetic variation with the six students and (2) Student 3’s genotype was heterozygous were supported by the data. As seen in Figures 1 and 2 and Table 1 with different agarose gel percentages, it can be concluded that each of the six students are genetically variable and…

Gel electrophoresis is a method used for separation and analysis of molecules such as DNA, RNA, and proteins, based on their sizes and polarity. DNA (deoxyribonucleic acid) is a molecule that carries most of our genetic information, and possesses a negative charge. During gel electrophoresis, DNA fragments can migrate through the gel also known as agarose when placed in a powerful electrical field. The rate at which the DNA fragments will move through the gel depends on their relative size.…

50 mL of 0.8% agarose gel is heated in the microwave for 1.5 minutes. The solution was cooled to room temperature and 5 μL of GelStarTM, a DNA marker, was added to the 50 mL. The DNA fragments will bind to GelStarTM and the GelStarTM enables the DNA complex to be visible under UV light. Carefully pour the solution into the gel-casting tray, insert the well comb and let the gel solidify. Place the gel tray so that it is parallel with the gel box and then fill the chamber of the gel box with 1XTBE…