Agarose gel electrophoresis

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    Gel electrophoresis is used to separate DNA, RNA, and protein molecules by using an electric field. Protein gel electrophoresis is similar to agarose gel electrophoresis, but runs protein bands instead. The technique of protein gel electrophoresis uses cross-linked polymers of acrylamide (which is a neurotoxin and requires careful handling) or polyacrylamide to separate proteins and small nucleic acids. This technique takes longer than the agarose gels used to separate larger nucleic acids. Also…

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    The technique used in this experiment was serum protein electrophoresis to determine the levels of proteins in cow serum. The objective of this experiment is to perform a gel electrophoresis of cow serum by agarose gel electrophoresis and to interpret the banding patterns produced by the migration of the serum. Electrophoresis is the shift of ions in an electric field in which the protein moves in the gel based on size as well as in electric charge. The electrophoretic mobility of small…

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    Vaccine Analysis Case

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    Discussion When comparing the banding patterns of the crime scene to those of the suspects, the resulting gel indicates that Suspect 2 was at the scene of the crime. Although enzyme 1 produced identical DNA fragments across the gel, enzyme 2 did not. This is evident in lane D and possibly indicates that this enzyme was unable to bind to recognition sites similar to the crime scene DNA in well B. Thus, it produced a DNA fragment smaller in size that travelled further. Since the DNA evidence in…

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    October 8, 2014 Nick D’Ambrosio, Matt Hermann, Carly Pruitt Title: Electrophoresis and Protein Weight Objective: The objective of this experiment was to gain a further understanding of gel electrophoresis, and to determine the denatured weights of unknown proteins using SDS gel. Hypothesis: If gel electrophoresis is conducted on unknown proteins, then the molecular weight can be determined by the distance travelled through the SDS gel compared to the standard. Methodology: The experiment…

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    Denaturing Protein Gels Kaytlin Goodwin Cedarville University Introduction Electrophoresis experiments are conducted on proteins for the purpose of separating proteins based on their characteristics. The characteristics that can be examined during the experiment are dependent on the preparation of both the proteins and the gel medium through which they will run. Native gel electrophoresis experiments highlight and examine multiple protein characteristics, such as size, charge, and…

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    the DNA from A17 and was stored at -20 oC until gel electrophoresis. Gel Electrophoresis To determine the success of DNA extraction and later PCR clean-up the samples went through gel electrophoresis. DNA extraction was tested by putting the samples in the loading chambers of the supporting matrix which was made of one percent agarose gel. GelRed, a nucleic acid dye, was added to help visually identify the DNA bands. A power supply was added to the gel for 20 minute by placing an anode…

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    To examine the DNA, a gel electrophoresis test needs to be run. The first step of gel electrophoresis is to set the gel, which is made of agarose. Agarose is used to separate DNA molecules, and acrylamide is used to separate proteins. The gel starts off as a liquid, which is poured into a molding tray and after it sets, it will turn into a solid. Next .all of the DNA needs to be collected and copied with a Polymerase Chain Reaction, PCR, which is vital to the success of the gel process because…

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    Phylogenic Analysis

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    quality using agarose gel electrophoresis. Species identification was performed by importing finalized CO1 sequences into two DNA databases to ensure accurate identification. Phylogenic analysis was then conducted to determine relationships between identified species. Electrophoresis Electrophoresis of DNA run in 2% agarose gel showed successful extraction of DNA in samples 1-8, 12 and 14-16. Samples were of good quality with high molecular weights (Figure 2). Samples 11 and 13 in Row 1 of gel 2…

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    (ade2::kan2R) cause the cell to become red and grow on mediums containing G418 or kanamycin resistant mediums was observed to have occurred. PCR and Gel Electrophoresis: Figure 1 is the product of gel electrophoresis containing the wild type and transformed PCR products, either being or not being cut by HindIII. From the left (reader’s left) of the gel to the right the lanes are; 1.Ladder, 2. transformed PCR product, 3. transformed PCR product cut with HindIII, 4. wild type PCR product, and 5.…

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    smaller parts. The fragments of DNA are then applied to a jelly-like substance called agarose gel, and an electric current is then sent through the gel. Because the strands of DNA are negatively charged, they will move across the surface of the gel, and the smaller pieces will move farther. The gel is hard to handle, so it is covered in thin nylon membrane, and a layer of paper towels that draws moisture from the gel. As the moisture is being transferred to the paper towel, the DNA is being…

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