Agaricus bisporus

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  • Fungi Lab Report

    Cellobiase (β-glucosidase) is a well characterized enzyme of the cellulose enzyme system which acts synergistically to degrade cellulose into glucose subunits (Ban152). The purpose of this experiment was to measure the rate of cellobiase activity in various club fungi types. The types of club fungi experimentally used was Agaricus bisporus (button mushroom), Pleurotus ostreatus (oyster mushroom), and Lentinula edodes (shiitake mushroom). The living environments of these three mushrooms are distinctly different. Button mushrooms are natives to grassland areas, oyster mushrooms are usually found on decaying trees and wood in clusters, and shiitake mushrooms are found on decaying trees. The hypothesis formulated was that the oyster mushrooms would have the highest rate of reaction, in comparison to shitake and button mushrooms, because it is found on decaying logs and it is larger in…

    Words: 917 - Pages: 4
  • Essay On Protein Bodybuilding

    activity test. Fraction 2 was further purified by subjecting to an ion-exchange chromatography column of DEAE Sephadex A-50. Elution condition was chosen as a stepwise salt gradient . Protein detection and determination: • Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed on the crud protein extract as well as those protein fractions collected from the two chromatography columns, using the method of Laemmli and Favere. • Molecular weights of the protein samples…

    Words: 1429 - Pages: 6
  • Km And Vmax Determination Of P-Npb Case Study

    Cent. Or. State Univ. Ext. Serv. 42. Henderson, A.M., Gervais, J.A., Luukinen, B., Buhl, K., and Stone, D. (2010) Glyphosate General Fact Sheet. Natl. Pestic. Inf. Cent. Or. State Univ. Ext. Serv. 43. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72 (1–2), 248–254. 44. Grabowski, G.A., Gatt, S., Kruse, J., and Desnick, R.J. (1984) Human lysosomal β-glucosidase: kinetic…

    Words: 2123 - Pages: 9
  • Leukocyte Gcrete Lab Report

    Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72 (1–2), 248–254. 44. Grabowski, G.A., Gatt, S., Kruse, J., and Desnick, R.J. (1984) Human lysosomal β-glucosidase: kinetic characterization of the catalytic, aglycon, and hydrophobic binding sites. Arch. Biochem. Biophys., 231 (1), 144–157. 45. Ašić, A., Bešić, L., Muhović, I., Dogan, S., and Turan, Y. (2015) Purification and…

    Words: 2582 - Pages: 11
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