Agaricus bisporus

Cellobiase (β-glucosidase) is a well characterized enzyme of the cellulose enzyme system which acts synergistically to degrade cellulose into glucose subunits (Ban152). The purpose of this experiment was to measure the rate of cellobiase activity in various club fungi types. The types of club fungi experimentally used was Agaricus bisporus (button mushroom), Pleurotus ostreatus (oyster mushroom), and Lentinula edodes (shiitake mushroom). The living environments of these three mushrooms are distinctly different. Button mushrooms are natives to grassland areas, oyster mushrooms are usually found on decaying trees and wood in clusters, and shiitake mushrooms are found on decaying trees. The hypothesis formulated was that the oyster mushrooms would have the highest rate of reaction, in comparison to shitake and button mushrooms, because it is found on decaying logs and it is larger in…

“chemoresistance” which produces drug resistant cells that renders the treatment ineffective. Since treatment is often difficult and unhopeful, extenstive research has been done on prevenative pathway. The inhibition of tyrosinase enzyme activity may result ininhit uncontrolled proliferation of melanocytes and prevention of accumulation of melanocytes and melanin pigments Representative animal cancer models are important for analyzing the underlying molecular pathogenesis of these cancers and…

INTRODUCTION The experiment studied mushrooms, which are members of the phylum Basidiomycota and the kingdom Fungi. The study tested three types of mushrooms: Agaricus bisporus commonly known as the button mushroom, Pleurotus ostreatus or the oyster mushroom, and Tricholoma magnivelare or the shiitake mushroom. The three types of mushrooms decompose different forms of organic matter and occupy different habitats. The oyster mushrooms live on tree roots and decompose dying hardwood trees; the…

activity test. Fraction 2 was further purified by subjecting to an ion-exchange chromatography column of DEAE Sephadex A-50. Elution condition was chosen as a stepwise salt gradient . Protein detection and determination: • Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed on the crud protein extract as well as those protein fractions collected from the two chromatography columns, using the method of Laemmli and Favere. • Molecular weights of the protein samples…

Cent. Or. State Univ. Ext. Serv. 42. Henderson, A.M., Gervais, J.A., Luukinen, B., Buhl, K., and Stone, D. (2010) Glyphosate General Fact Sheet. Natl. Pestic. Inf. Cent. Or. State Univ. Ext. Serv. 43. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72 (1–2), 248–254. 44. Grabowski, G.A., Gatt, S., Kruse, J., and Desnick, R.J. (1984) Human lysosomal β-glucosidase: kinetic…

Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72 (1–2), 248–254. 44. Grabowski, G.A., Gatt, S., Kruse, J., and Desnick, R.J. (1984) Human lysosomal β-glucosidase: kinetic characterization of the catalytic, aglycon, and hydrophobic binding sites. Arch. Biochem. Biophys., 231 (1), 144–157. 45. Ašić, A., Bešić, L., Muhović, I., Dogan, S., and Turan, Y. (2015) Purification and…