Agar

elegans were placed on a 10 NGM-lite plate (3g/L NaCl, 17g/L agar, 2.5g/L peptone, 1mM CaCl2, 5μg/ml cholesterol, 1mM MgSO4, 25mM KPO4 buffer pH6.0) which contained 0P50 E. coli, one week prior so that they would reproduce. Two different stages of development were found on the plate, adult, and larva, and 2-5 worms…

After receiving unknown organism #14, many tests were performed to discover the identity. After running a variety of tests, my results led me to believe that my unknown organism was Klebsiella pneumoniae. K. pneumonia produced a negative-rod gram stain. My organism, K. pneumoniae, tested positive for the three main carbohydrates, glucose, lactose, and sucrose. After running tests on these carbohydrates, a pH indicator color change from red to yellow signified a positive result, while remaining…

The Ampicillin Effect of Escherichia coli in a Beta Lactamase plate and non Beta Lactamase plate. Jimena Cárcamo Lab 4 Concepts in Biology 173 C October 6, 2014 INTRODUCTION Bacteria can be conserved in a lab in many different ways, but the most common way is a suspension culture. A suspension culture is when the cells are grown in a sterile, full of supplements, broth. The broth allows the cells to divide and grow easily and will later turn into broth turbid. When culturing…

As the writer gathers his thought to fill the paper of his joy of freedom and agony of twelve years of slavery with the lifeless ink dripping from his pen. His thoughts travel to a past, past filled with promises of liberty and freedom to peruse his dreams. Later became a person of agony, family less and striped of his freedom to serve as slave known as Solomon Northup. Solomon Northup, born in a family of slave in July of 1808 in the town of Minerva, Essex county, New York, which became free…

utilized to help identify an unknown microbe. For the first part of this experiment, a dichotomous key (attachment 1) was created using the information of possible microbes for the unknown as provided by the instructor. The student was then given an agar slant tube containing the unknown #4 microbe to use throughout the course of the experiment. Identifying the shape and whether the microbe was gram negative or positive was done by performing a gram stain and viewing it with a microscope.…

Plant Material Collection Fully grown whole plants of Tribulus terrestris Linn. were collected from different localities of Chennai, Tamilnadu. The fruits were separated out from the plants and washed in running tap water to remove adhering dirt and soil particles and dried first under shade in room temperature and secondly in a hot-air oven for complete drying at 50-60˚C. The dried fruits were made into coarse powder by grinding and stored in labelled bottles for further study and…

Safety standards within a laboratory should be designed in a way that eliminates risks due to using potentially dangerous practices or procedures and dangerous materials. All laboratories are potentially dangerous places, and it is the actions and attitudes of those working there that determine their safety and that of their colleagues and ultimately of the whole community. The manual identifies the biological agents that are in use within the laboratory. It also points out the exposure…

The synthesis of Aa-g-poly(MAA) involve the optimization of various reaction conditions like initiator concentration, monomer concentration, crosslinker concentration, solvent concentration and pH. After the optimization of various reaction parameters the replications were carried-out in triplicates for the reproducibility of the results and statistical of the results obtained. It was performed using the Statistical Package for Social Science (SPSS) version 10. Statistical results of the…

media types that contained selective ingredients. By cross-referencing the different medias and comparing observations, the generalized groups of contaminants were able to be confidently conjectured. Growth was witnessed on DRBC, DRBC + CuSO4, and MRS agars. The finished beer sample was likely contaminated with lactic acid bacteria and wild yeasts such as Brettanomyces sp., based on the colony morphologies witnessed on these respective plates. Introduction The…

organism responsible for this patient’s disease. There are several reasons, but the one that stands out the most is that this organism can only grow on buffered charcoal-yeast extract, which is why there was no growth present on the 5% sheep blood agar. Other contributing factors which make it well-defined that this organism is the agent of disease are that the symptoms the patient had go along with what was exactly listed for the disease as well as the fact that Legionella pneumpohila often…