Agar

deduce their role in disease states. The inaugural step in the process of bacterial identification is the culturing of the sample followed by primary isolation, and hence is limited by the fact that the organisms in question must be capable of growth of agar. Procedures for identification are largely dependant on phenotypic analysis, including colony morphology and cellular characteristics. One such defining element of bacterial identification is the Gram stain, which is critical knowledge for…

Different batch experiments were conducted to access the effect of pH on removal of heavy metals by T1B1 strain. 100 ml of sterilized SE medium was inoculated into 250 ml Erlenmeyer flask containing 100 µl (107cells/ml) of bacterial suspension with HM (mg L-1): Fe (150), Mn (100), Ni (50), Cd (30), Cu (30) and Zn (50) with pH adjusted to 4, 6.5 and 9. Another set of experiments were set up by inoculating SE medium containing HMs (mg L-1 ): Cr+6 (100, 150, 200), Fe (100, 150, 200), Ni (50, 100,…

As I remember from the start of my early age days, I was fascinated by the concept of the biology, how the human body works, and how disease process takes over slowly and affect human body and how can we alter the development of disease, has always attracted me, so I choose my career to become doctor and serve humanity. Trying to help needy and poor people is always been my passion and medicine is one of the few profession where i can serve the humanity with my own hands. When i see my patients…

(Move to treatment??) Researchers have discovered that the fight against invading B. anthracis begins with the first infected cell. They found that the impacted macrophages will instantly communicate with other immune cells to warn them of the distress and develop a strategy in order to survive. The key signaling molecule that is involved in the survival strategy is adenosine triphosphate or ATP, which is a basic currency of energy transfer used by all organisms for various biochemical processes…

B Sridhar et al (2012) studied Purification and Characterization of Biosurfactants. The emulsifications of biosurfactants were checked in various oils such as olive, coconut, sunflower and rice bran oils, the emulsification activity of biosurfactants. Pseudomonas aeruginosa had higher emulsification activity than Saccharomyces cerevisiae. While checking the sugar and lipid content in biosurfactants by phenol sulphuric acid method and vanillin reagent method, found high in Pseudomonas aeruginosa…

Question 1: pH can be described as a measure of acidity or basicity (Alkalinity) of a solution. It is exactly equal in amount to the negative log of the concentration of hydronium(H3O+) ions. Due to the negative log relationship, increased hydronium ions transform to lower pH. When we add it to water, an acid releases hydrogen ion. Hydrogen ions in water unite with the water molecules to form hydronium ions, thereby lowering the pH and increasing the acidity of water. Water exposed to air is…

areas of research. Isolation of microbes from a mixed culture broth was performed with the finding of two different microbes; Escherichia coli and Sporosarcina ureae. The isolation process was performed by streaking a mixed culture onto a nutrient agar plate, then transferring the isolated cultures after incubation for biochemical testing. S. ureae is discussed below. Biography S. ureae is a coccus, gram-positive, endospore-forming, chemoorganotrophic, obligate aerobe and has motility via…

pyocyanin, an antimicrobial phenazine pigment. For this study different clinical specimen were collected and from these P. aeruginosa was isolated. To isolate good pyocyanin producing P. aeruginosa, repeated plating of all the isolates on cetrimide agar helped in final selection of the 5 isolates. To examine the antimicrobial activity of those isolates, in vitro inhibition by cross streak assay was performed, in cross streak method all the 5 isolates were able to inhibit the growth of…

For a culture test a endocervical or urethral swab specimen is needed. Specimen can also be taken from the rectum and pharyngeal. The specimen is streaked onto a Thayer-Martin or Martin Lewis selective medium. It can also be streaked onto a chocolate agar nonselective medium and incubated at thirty-five Celsius to thirty-six Celsius and with a five percent of carbon dioxide. A gram stain and oxidase test is then preformed and positive results will show a gram negative, oxidase positive…

Figure 1a. Size of nano curcumin. Image of curcumin nanoparticles taken with Transmission electron microscope (JOEL 2100F) showing particles having size distribution of 59.9 nm to 198 nm ( average size of 160 nm). Figure 1b. Bioavailability of nano curcumin. Mice were given I.P injections with curcumin/nanocurcumin (vehicle: PBS containing 10% ethanol) per mice at 100 mg/kg body weight and then bled at different time points via retro-orbital puncture and blood collected from each…