Agar

For this reflection, I’m going to specifically focus on the morphological unknown lab we used 2-3 lab periods for. Before going into lab, I started to create my own dichotomous key for all the microbial species we’ve been working on, assuming we’d have a species we previously worked on. My dichotomous key went from from gram stain to respiratory use and to fermentation results. I quickly realized that I shouldn’t have been working on this as my first go to. I mentioned in a past reflection that…

Now days, microorganisms are very important to identify to Genus and species because they can cause various diseases. We need to know that what helpful or harmful microbe in this environment. This project’s purpose is to identify an unknown bacterium by various methods. Most stains used in microbiology are differential stain, which use more than one dye so that different cells, chemicals, or structures such as Gram staining, TTC reduction, Oxygen requirement test, Catalase test, Nitrate test,…

In this chapter Edgar has a lot of hate for Sir Phineas. Some of the reasons are Sir Phineas stole Edgar’s book and hide it from him. Another reason is Sir Phineas “Killed Edgar’s dad.” When Edgar and Sir Phineas got in the fight Samuel stood up for Edgar and got his book back. One reason Edgar and Sir Phineas are not a good mix is because Sir Phineas stole Edgar’s book that he found when he was climbing up to the Highlands. For example, on (Page 36) Edgar said “I know what you did to…

Silver nanoparticles have unique antimicrobial properties. They have a variety of applications: diagnostics, wound healing, pharmaceuticals, etc. These nanoparticles destroy bacterial cells walls and interrupt membrane integrity by their electrostatic attraction to the negatively charged bacterial cell walls [10]. Metal nanoparticles are usually synthesized by chemical techniques which is costly and highly toxic. Currently, there is a growing demand for alternative methods that would limit the…

Scientific experiments and observations have led to many discoveries, which in turn have allowed scientists to develop a better understanding on the way diseases originate in humans. Molecular biology in particular has allowed scientists to discover the way in which domains, found in proteins impact a genome. Domains are autonomous folding units that carry out specific functions and mechanisms in a certain protein. These functions consist of being responsible for interactions in the cell, which…

A small number of E. coli was put into culture tubes. After it grew for a while, experimenters placed equal amounts of the cultures onto agar infected with the virus called T1 phage. A researcher hypothesized that if changes occurred in response to contact with the selective agent, survivors would be allocated according to a Poisson distribution and the mean would be equal to the variance…

“Clostridium perfringens is a nonmotile, encapsulated, endospore-forming, gram-positive rod. It is classified as an anaerobe since no colonies arise when agar plates streaked with this bacterium are incubated in the air.” (University of Pittsburgh School of medicine, Par 2) Clostridium perfringens are bacteria that produce toxins harmful to humans. This bacteria are found anywhere in the environment. Basically clostridium perfringens are food poisoning bacteria. Clostridium perfringens is a…

S- One of my strengths is that I made my topic very relatable, and everybody was interested in my warm up. The members were fascinated by the agar slides and where I went to contain the samples. They kept asking questions about the places that I obtained the bacteria and some seemed amazed at which slide corresponded with which place. It is important to have a compelling group to encourage active participation and learning about the group topic. Another action that I viewed as one of my…

DISCUSSION Unknown #297 was collected from a urine sample from a patient with a kidney infection, named Doris. There have been several tests that were performed at the University of Illinois clinical Laboratory to identify the unknown. Unknown #297 was identified to be Bacillus subtilis with the various tests that were conducted. Most of the results that were observed in the laboratory matched the expected results for the bacterium. All of the morphological characteristics results matched…

cycle, oxidative phosphorylation, and biosynthetic pathways because they have small base pairs. Snowden explained bacteria cannot survive outside of body fluids and it can only be transmitted by sexual contact. This bacteria cannot be seem visible on agar plate meaning it cannot be grown culturally in a…