Agar

INTRODUCTION : Ghee is a class of clarified butter that originated in South Asia and is commonly used in South Asian (Indian, Bangladeshi, Nepali, Sri Lankan, and Pakistani) cuisine and ritual. Ghee is a type of clarified butter that is prepared by boiling butter and removing the residue. Spices can be added for flavor. Ghee has a long shelf-life and needs no refrigeration if kept in an airtight container to prevent oxidation. The texture, color and taste of ghee depends on the…

If there is coliform in the water sample, the color will change from purple to yellow, indicating that the coliform bacteria ferments lactose. The next test that is used to analyze is the EMB agar test, a differential and selective media designed to distinguish the colonies of different microbes and encourage the growth of the desired microbes, while inhibiting the growth of unwanted microbes. Due the media’s composition, it can distinguish…

Pseudomonas aeruginosa can be cultured on; • MacConkey agar • Blood agar • Luria Broth (LB) and Luria Agar (LA) Media • Cetrimide Selective Agar • Tryptic Soy Agar • Cystine Lactose Electrolyte-Deficient (CLED) Agar…

Unknown #380 was identified as Serratia liquefaciens and this information was received from the patient, Doris. This unknown is a part of the Serratia genus according to Brown’s Microbiological Application1, is a gram-negative rods that are usually found in the digestive tract. The data was gathered by isolating the organism and various tests were carried out, and observations from scientific literature were used to affirm that the organism is S. liquefaciens. Most of the tests carried out…

BACTERIAL TRANSFORMATION By Derek Tang BIO 281 Lab section 80243 Abstract: The objective of this experiment was to observe the results of bacterial transformation in various growth conditions and determine how successful the altered organism is after the transformation. The organism used was E. Coli because singular cell organisms can have a new gene more easily inserted than multi-cellular organisms. The gene which was inserted is pGLO that carried the gene encoding GFP and a gene for…

Identification of Human Streptococcal Pathogens Materials 1. Glass microscope slides 2. Bunsen burner 3. Inoculating loop 4. Sharpie pen 5. 2 bacitracin discs 6. Sterile saline tubes 7. Sterile cotton swabs 8. Tongue depressors 9. Gloves 10. 1 blood agar plate/student pair Procedure Divide a blood agr into two sections, one for you and the other for the lab partner. Wash hand thoroughly before proceduring to the getting a throat culture. Depress the tongue with a sterile depressor, donot put it…

For the culture media tests, synthetic medium, tryptic soy broth (TSB), SF medium, MacConkey agar, and nutrient agar and glucose were used to detect if specific organisms would grow on each media. Synthetic medium is defined media and the contents are exactly known. A positive result would be if one could see cells within the medium after incubation, and…

pGLO plasmids in an attempt to build up ampicillin resistance and to be able to observe the colonies glow bright green. In proper conditions, bacteria that have successfully transcribed the +pGLO plasmids are resistant to the ampicillin within the agar gel. If the transformed E.coli bacterium are in the presence of arabinose, they form colonies that are able to glow green when held under UV light. The bacterium in the…

Illustration of sexduction in E. coli using streptomycin resistant F-lac- recipients and F′lac+ donors Abstract The process in which DNA from a donor cell is transferred through physical contact into a recipient cell is known as conjugation. In the experiment, sexduction, a cross between F′ and F- plasmids, was demonstrated by mixing the donor and recipient, diluting them and using selective plating methods. The plasmids from the donor, recipient and transconjugant were isolated and analyzed…

the expected results. The expected result for fermentation of glucose test was negative while the observed result was positive. Conversely, in Kligler iron agar media, which exams the ability of bacteria to ferment lactose and glucose, M.Luteus gave negative outcome which matches the expected result. Thus, I repeated the Kligler iron agar to test if M.Luteus can ferment glucose and I obtained same result which is negative. Getting positive result for glucose in beginning could be…