swab to gather the Propionibacterium acnes (P. acnes) bacteria from various spots around our face, we then grabbed a Petri dish filled only with a thin layer of agar. We proceeded to swab the P. acnes bacteria off of the cotton swab (gently to not break the agar) into the Petri dish. Once we had completed swabbing the P. acnes over the agar we closed the Petri dish, taped over the dish and then wrote our initials on the dish and handed both to Mr.Breines who then put the Petri dishes into an…
microbiota or other contaminants via plate streaking on four selective and differential media namely: Mannitol salt agar (MSA) to inhibit the growth of most organisms and differentiate possible pathogenic staphylococci, Phenylethyl alcohol (PEA) agar to grow gram-positive organisms, Eosin methylene blue (EMB) agar to cultivate gram-negative bacteria and isolate fecal coliforms, and Nutrient agar for a general purpose to support the growth of the unknowns. All plates were incubated at 35˚C for…
and Lee, 2012) Some errors were that the music playing could have been not exactly the same, the agar that was poured at the beginning of the experiment could have been poured unequally, and the bacteria could have been distributed unequally. Some improvements that could have been done to the experiment were a longer growing time, having more varieties to the music, and measuring the amount of agar into each petri plates. Both table one and figure one have shown that no music grows the most…
Ultra-violet light is produced by high-energy wave frequencies just outside of the visible spectrum. This light energy can interfere with the bonds which comprise the backbone of DNA by exciting the electrons in these atoms—leading to conformational changes which cause base-pair bonds to be broken, shifted, or replaced, causing genetic mutations, or abnormalities in the typical phenotypic expression of an organism. The purpose of this lab was to understand more about ultraviolet light as an…
Science Enterprises have petri dishes agar already setup to buy. The site also has recipes for making your own agar. The easier route would be to buy agar setup, and you would get more accurate results. Science Enterprises then says to store petri dishes in the refrigerator for up to sixty days as long as they are upside down. When you are ready…
2.4 Factors that Affect Colletotrichum sp. Infection and Growth Factors that affect Colletotrichum growth and infection include inoculum concentration, wetness duration, temperature, age of plant, resistant plant cultivar, pH, nutrient and light. 2.4.1 Inoculum density One of the main factors that facilitate Colletotrichum sp. infection and growth is inoculum density. Up till now, according to Diéguez-Uribeondo et al. (2011), disease severity increased exponentially with increasing…
He Thinks He Can There once was a little train engine that could. There also once was a man named Edward Pierce that could do more, by robbing the train. He did it because he simply believed he was able to achieve his goal. Edward Pierce was an opportunist and deemed himself capable of pulling off the “largest crime of the century” because he had all the necessary assets including money, intelligence, and connections in the underground world. Edward Pierce’s method of acquiring money is…
noticed that the arrangement appeared to be single celled. I also put the bacteria on a streak plate. On the second day, I did another gram stain and the bacteria were again pink and bacillus or rod shaped. I also observed the bacteria on a nutrient agar plate and noticed that the bacteria were light white almost clear color bacteria. I took the bacteria that were…
Many products in Ayurveda have antimicrobial activities against many pathogens. Sindur which is a kulpirasayan also is a broad spectrum antibiotic. Antimicrobial activity of tal sindur : Metallic and mineral preparations are unique preparations in Ayurveda. Along with herbal preparations which includes Bhasmas, herbo-mineral preparations, Kupipakwa Rasayanas which are inorganic mineral preparation, are the base of treatment of various diseases in the Ayurveda. Tal Sindoor contains mercury ,…
Introduction: Antibiotic sensitivity testing is used in clinical laboratories to predict the effectiveness or failure of antibiotics in vivo by testing in vitro. The need for antibiotic sensitivity testing is urgent due to the surge of antimicrobial resistant pathogens in recent years. Antimicrobial resistance hinders the prevention and treatment of the infections caused by parasites, bacteria, fungi and viruses. Antimicrobial resistance is the resistance of a pathogen to an antimicrobial that…