Agar

dried in an oven. The bacterial suspension were prepared of bacterial strains and they were equivalent to 0.5 McFarland (108×1.5 CFU/ml). Then, a uniform cultivation was done by sterile cotton swabs soaked in suspension on the surface of Mueller-Hinton agar (Merck, Germany). Then, the disc containing the extract with concentrations of 500 μg/ml, 250 μg/ml and 125 μg/ml, was removed with sterile forceps and placed in a certain distance from each other, from the edge of the plates, on the surface…

experimenting will need goggles, gloves, and an apron. To do the project, you will need nine glass petri dishes (make sure you get them sterile), agar, beef broth, sterile cotton swabs, three slices of bologna, three slices of ham, and three slices of turkey. Experimental errors would be breathing on the plate of agar, amount of agar, and water on the plate of agar. In conclusion, the results did not support the hypothesis. The bologna grew an average of 15 total colonies and an average of two…

Before inculcating the TSA plate, the surface of the agar and sides of the plate were carefully examined for any visible contamination. A bacterial culture was obtained and a sterile non-toxic swab was gently inserted inside the tube rotating around the culture. The swab was removed by carefully pressing the inside wall of the tube, removing any excess inoculum from the swab. The plate was inoculated by streaking the swab over the entire agar surface in three directions. The plate was rotated…

Jaqueline Moreira Biology 23 March 8, 2016 Pseudomonas aeruginosa Introduction P. aeruginosa belongs to the family called Pseudomonadaceau ( Willey, J., Prescott Microbiology, 2014, p.527). P. aeruginosa is a gram negative bacterium. The shape of this particular bacterium is straight or slightly curved rods, but not helical. The size of this bacterium is about 0.5-1.0 micrometers wide and 1.5-5.0 micrometers long. The arrangement is single, but as is starts to age it forms cysts. P.…

should draw four equal quadrants and label them c (control), 1, 2 and 3, as in the example photo below. Be sure to make note of which antibiotic 1, 2 and 3 correspond with before moving on and repeating these steps on the second agar dish. Following this, on the side of each agar plate, make a label indicting your teacher name, period number, lab group and bacteria sample, this will allow you to stay organized throughout the…

to streak agar plates to culture any microbes collected in the swabbing process. The microbe-exposed plates were then sealed and incubated at twenty-eight degrees Celsius for one week. Without environmental disturbances or nutritional competition, species growth was only restricted…

antibiotics. These bacteria have grown resistant to certain antibiotics and in this experiment, we tested how S. marcescens reacted to 6 different antibiotics. My colleagues and I swabbed a mixture of nutrient broth and Serratia marcescens onto an agar plate containing 6 antibiotic tablets and let the plate sit for a week. We then measured each tablet's zone of inhibition (ZOI) and pooled our data together. It was determined that the bacteria were most resistant to Ampicillin with an average ZOI…

Triple Sugar Iron Test In triple sugar iron slant, the protocol of Acumedia Manufacturers Inc. (2010) will be used. A loopful of isolate will be inoculated. It will be stabbed into the slants to the bottom and will be streaked at the surface of the agar slants. The slants will be incubated for at least 18 to 24 hours at 37°C, and the results will be recorded afterwards. 4. Oxidation-Fermentation Test (OF Test) In OF slants, a loopful of culture will be stabbed halfway to the bottom of the…

The total proteins are composed of 12.8 grams, while cholesterol at 13.8 grams for every 100 grams of fertilized duck egg (Table 2) (Jalaludeen & Churchill, 2006). Proteins aid in the cultivation of various bacteria whereas it is one of the basic growth components in bacterial cultivation. Moreover, the presence of cholesterol in vivo aids in nurturing bacteria and allow it entry into host cells. (Tille, 2013). Table 2 Nutritive Value of Duck egg compared to Chicken egg.…

CHAPTER-2 VARIABILITY AMONG THE ALTERNARIA BRASSICAE ISOLATES INTRODUCTION The chapter is mainly concerned with examining the variability among Alternaria brassicae isolates from the oilseed brassicas. Since, the oilseed brassicas are more prone to the fungal attack and thereby, resulting in reduced productivity of the oilseed. Therefore, the present investigation focuses on the studying the features such as morphological, pathological and molecular variability of Alternaria brassicae. The…