Agar

me continuing on ahead doing the catalase test she had recommended that I do a mannitol salt agar test or a Blood agar test for my final test, which would help determine between Streptococcus pneumoniae, Staphylococcus epidermidis. I decided to go along with the mannitol salt agar method and again with my inoculated loop I grabbed some of my unknown #15 and I had applied it to aseptic technique to the agar plate. While waiting for my results to appear, this test will show us whether or not the…

Bacteriological analysis of 120 soil samples from dumpsites in Ojo, Lagos, with standard microbiological techniques revealed the total viable aerobic bacterial count as 2.19 x107 cfu/mg soil on eosin methylene blue( EMB) agar and 5.12 x 108 cfu/mg soil on MacConkey (MCC ) agar, indicating that the bacterial count is higher on MCC than on EMB. The bacterial isolates and their frequency of isolation from dumpsites were Escherichia coli (33.3%), Klebsiella spp.(16.7%) ,Pseudomonas spp. (13.3%),…

stain tests were performed in order to identify which unknown is gram positive and gram negative. Using selective and differential media, like MacConkey agar which allows the growth of gram negative bacteria only that are able to ferment lactose. Also, mannitol agar was used which isolates and detects gram-positive bacteria. As well as blood agar, which is a nutritive media with differential properties in respect to hemolysis. The catalase test indicated catalase production and the coagulase…

To determine the unknown’s morphology is the first step in narrowing down possible organisms. A MacConkey and Mannitol Salt Agar tests were conducted on the organism and revealed the following. No growth on the MacConkey plate resulting in a gram positive bacteria and the Mannitol Salt Agar result showed it could be Staphylococcus or Micrococcus. (1) A gram stain test was used in determining the shape, size and arrangement of the unknown bacteria (1). The unknown organism is heat fixed onto the…

inhibition to lowest. These predictions that was base on previous experiments from (Mcbride et al 2007) and (Wendelbo et al., 2003) were falsified. There is however limitation to my experiment because of the small sample size that consisted only of five agar trays. If more trials were done, we may get results closer to the sources. Since erythromycin is being supported by my data to be a more effective antibiotic, doctors can prescribe this antibiotic to patients with…

contained no preservatives. Once the experiment is complete, the bacteria dishes should be soaked in a mixture of bleach and water and gloves and goggles should be worn during this process. Procedure Prepare Agar Plates by heating a mixture of 1 Liter purified water and 23 grams Nutrient Agar powder for 15 minutes on a hot plate at 121ºC while stirring consistently. Once the mixture is clear, pour it into the petri dishes one at a time by just barely covering the surface of the plate. Set…

Discussion The various test that were completed had supporting evidence that deduced the identity of the unknown microorganism. A gram stain was performed on the bacterium and a purple color was seen under the microscope. The purple hue that was acquired by the unknown microorganism indicated that the organism had a thick peptidoglycan layer meaning that the bacterium was gram positive. Looking under the microscope helped to identify the cellular arrangement of the bacterium which was sphere…

Nanoparticles 1. Punch 15 circles from a filter paper with the hole punch. 2. Using clean tweezers, place three filter paper circles) into each baby food jar (#1 through #5). Let them soak until you need them in step 10 3. Lay out five of the nutrient-agar prepared media plates. Place the plates upside down on the clean counter or table and use the permanent marker to label the back of each one, #1 through #5. 4. After labeling them, flip the plates over so that the lid is on top. 5. Take off…

Water Crystal Violet Alcohol Safranin Iodine Methylene Blue Hydrogen Peroxide Reagent A (sulfanilic acid) Reagent B (dimethyl-alpha-napthylamine) Kovacs Reagent EMb plate Blood plate MSA plate Starch plate Litmus milk tube Nutrient agar Slant…

placed in agars with ampicillin and without ampicillin. The agars are later inverted and incubated at 37ºC. After a certain time the results are analyzed. Two alternative hypotheses made in this experiment are: 1) the presence of ampicillin will have an effect on bacteria with plasmid 2)there will be a difference in bacterial growth between plasmid lux and puc18. Some predictions are: 1)The agar plate with ampicillin and bacteria without plasmid is going to show no growth. 2)The agar plate with…