Agar

preheated bath, where the cell is heat shocked, allowing the plasmids ' to enter the cell at a rapid pace, once the cell expands. After the tubes are incubated, they were transferred to six agar plates. There were two types of agar plates, one included the plasmid lux and the control plasmid pUC18. Every agar plate was labeled independently…

First the unknown tube was isolated. This was completed by taking an inoculating loop and spreading the unknown out on to a Nutrient Agar plate. This was done using the streak plate method, which is described in the lab manual. The bacterium were incubated and grown at 48 hours. Upon observation the isolation plate showed growth of two different colonies. After the morphology was observed and recorded, a Gram stain was performed on each of the two unknowns, this procedure is found in the…

Streptococcus pneumoniae is placed in a blood agar plate, Penicillin will prevent the bacteria’s growth, while Tetracycline and Kanamycin will have no effect. Therefore, if Streptococcus pneumonia is given one week to grow, then there will be a 1 mm zone of inhibition around Penicillin and a 0 mm zone of inhibition around Tetracycline and Kanamycin. Materials and Methods Prior to beginning, the work stations were cleaned and gloves were placed on. Blood agar plates were labeled and two…

Then, we looked at the Tributyrin agar and looked for a clearing/halo around the bacteria. After this procedure, we inoculated three urea broths and after storing it at our optimal temperature for 48 hours we brought it out and examined them for color change. After these tests had been…

As a negative control, the test strain should be inoculated alone. Inoculate in the centre of the plate (V8 juice agar) or Eucalyptus tree and incubate at 25oC in the dark for two weeks. The plate or bark is observed periodically for the sexual development i.e perfect state. Mating can be observed by seeing the dense filament growth surrounding the colony and is confimed…

Purpose: The purpose of this lab was to compare the effect of using soap (antibacterial and non-antibacterial) and no soap on the growth of Escherichia coli (E.Coli). Hypothesis: If anti-bacterial and non-antibacterial soaps are added to a standard agar media before the E.Coli was introduced and incubated, then the portion of the plate containing the antibacterial soap will have the least amount of bacterial growth because the antibacterial soap contains a higher percentage of bacteria-killing…

CHAPTER 3 3.0 Introduction To establish the differences in consumer acceptance, the pork liver polony sample was compared against the Colcom standard polony. Experiments were conducted by manipulating the independent variable, adding pork liver in two of the polony treatments, and observing differences between the test samples and the control sample. Analyses were used to quantify and describe the differences in shelf life, sensory evaluation scores, microbiological analysis and proximate…

The results indicated in Figure 9.1, represent how in the six agar plates obtained, three of them had ampicillin, while the other three had no ampicillin.…

2.0 Introduction Consumption of foods and beverages that encourages a healthy living, better health and lowering the risk of sickness has become very popular among the consumer whom are health conscious. Hence, a lot more attention is paid to different food extracts such as green tea extracts, grape seed extracts and ginger extracts as they posed excellent antimicrobial, antioxidant and anti-carcinogenic properties (Martin et al. 2009). These extracts have great potential in improving the…

-Antibiotic disk of gentamycin, novobiacin and penicillin -Nutrient agar in petri plate. -Metric ruler, markers and plastic tweezers. -Tea candle light, sterile swab, rubbing alcohol and bleach -Other includes personal protective equipment like an apron, gloves, and safety goggles. Kirby Bauer diffusion is widely used test…