Agar

ampicillin resistant. Thus by transfection of the DNA, E. coli was able to take up the strain into its genome and recombination occurred as the cell became ampicillin resistant. That is why colonies were able to form on the agar plates. All the experimental plates had colonies including the agar plate of the enzyme of DNAse. Which simply contradicts the transformation principle, this effect could arose by systematic errors that have occurred during the experiment, that is, the enzyme DNAse was…

are slightly raised or flat with irregular margins (Dworkin et al., 2006). On MacConkey’s agar, E. aerogenes will grow, as it is gram negative, and will appear pink as it can metabolize lactose; in blood agar it will grow using gamma hemolysis, causing no change to its color or lysing the blood cells on the plate (Bergey et al., 1957). E. aerogenes can grow in both glucose minimal media and Simmons citrate agar, showing that it can use glucose and citrate, respectively, as its only carbon source…

was done it reveal a gram positive cocci result. A streak plate was completed revealing that the microbe was round/filamentous, convex, erose, granular, yellow, and opaque. The next test was the Tryptic Soy Agar plate for color. Followed by the oxidase test and last was the Mannitol Salt Agar test. Thus microbe #1R was Sporosarcina ureae (Brady, Personal Communication). Procedure of the Gram stain Purpose: The Gram stain reveals whether a microbe is gram positive or…

test how the ecology of life works, we measured and reviewed the growth of bacteria after washing one’s hands at different intervals of time. After washing our hands four times in 30 second intervals, we wiped a finger on a different segment of an Agar plate where our bacteria would have a strong environment to grow and develop. To be sure that each individual whose’ hands would be tested had a source of bacteria on them, we had the freedom of choosing any object in or around the school before…

MATERIALS AND METHODS The first day of the independent experiment was on October 25, 2016. Mannitol Salt Agar and Phenylethanol Agar are selective media that were used to isolate M. luteus from the environmental samples. Sterile cotton swabs were used to obtain the samples from the skin, arms and dust through wiping the surface of the sample source. A handful of backyard soil was previously diluted and then 2mL of this diluted soil was transferred to a sterile test tube containing 9mL water…

We were given three agar plates by our TA, each agar plate containing a nutrient broth. We labeled each agar plate with whatever content we were going to put inside of it. We worked with the control so our three labels were “LB^NP, LB^AmpC, and LB^C”. LB^NP means the broth with no plasmid. LB^AmpC means the broth…

Initially, it started with three plates, the Tryptic Soy Agar (TSA), the Colistin-Nalidixic Acid agar (CNA), and the MacConkey plate (MAC). Each plate had growth of coccus-shaped colonies. Through Gram staining, it was observed that the colonies on the TSA plates contained both gram-negative bacteria and gram-positive bacteria.…

incubator for at least 24 hours. This is done to allow the agar plate to get used to the environment and also to allow a zone of inhibition to be seen on the agar plate if the antibiotic is…

culture. Sterile techniques were followed while performing precise instructions as stated in the referenced Laboratory Manual. Example 1: The first procedure performed was done by isolating a pure culture from the mixture onto a solid Trypticase soy agar (TSA) media. Sterile technique was done by flaming the loop until it turned red to ensure that there were no current bacteria on the loop avoiding contamination followed by rapidly flaming the neck of the test tube to prevent the entry and…

inside our lab room. We then opened a petri dish and smeared the swab across the surface of the agar several times. Once the bacteria was on the agar, we closed the lid immediately, hoping to prevent microorganisms in the air from contaminating our sample. We also collected a sample from the skin of a lab member, and another from the microorganisms in the air. Once our samples were placed in the agar,…