Agar plate

This results suggest that an evolutionary change has presented itself on the antibiotic plates due to the original bacteria being susceptible to it. With the bacteria having a short generation time of 45 minutes, the chances that a mutation would arise with the resistant plasmid was highly likely. Through conjugation the bacteria would then…

glow. The plate with -DNA/+Amp grew and didn’t glow; however, the cells in that plate shouldn’t have grown or glowed because only cells with +DNA contained the pGFP. In the plates labeled +DNA/+Amp and +DNA/+Amp/+IPTG nothing grew or glowed despite the presence of ampicillin and the glowing gene, which means the bacteria did not pick up either of the genes. In the experiment, the resistant ampicillin gene and the gene for green fluorescent protein was tested. After incubating the plates, it…

stain and mount the samples we grew. Of course it taught us how to view our stained specimen under a light microscope. Grow the microorganisms we obtained was the first part of the lab. What we used to culture our microorganisms was a Nutrient Agar Plate. Its general purpose is to be a nutrient medium used for the cultivation of microbes. It is frequently used for isolation and purification of cultures. We used the streaking method. Streaking is a technique used to isolate…

gram negative bacteria. There are media that will be useful in isolating this organism. The best option for a gram negative bacteria would be using a selective media specifically MacConkey agar or EMB agar. These agars are used to isolate gram negative bacteria because the crystal violet in the MacConkey agar will not allow the growth of gram positive bacterium making it a good media to isolate Desulfovibrio. If that selective media does not work then another media is to use a sulfur-reducing…

This study is about isolating phage. The first step was to do an enrichment isolation then do a plaque assay and spot test. After that the next protocol was do a plaque streak, which was conducted numerous of times. Then serial dilution was conducted and RE Digests of Phage DNA as well. Last step was protocol 10.3, gel electrophoresis. The relevance of this study is important to field of biology because bacterial infections could be treated with bacteriophages. Bacteriophages can be a solution…

L.B. Agar Lysogeny broth (LB), a nutritionally rich medium, is primarily used for the growth of bacteria. The abbreviation is commonly taken to mean Luria broth, Lennox broth, or Luria-Bertani medium. According to its creator Giuseppe Bertani, the abbreviation LB was actually intended to stand for lysogeny broth (1). The formula of the LB medium was published in 1951 and continues to be the most common media used today (2). There are several common formulas of LB. Although they are different,…

Triple Sugar Iron Test In triple sugar iron slant, the protocol of Acumedia Manufacturers Inc. (2010) will be used. A loopful of isolate will be inoculated. It will be stabbed into the slants to the bottom and will be streaked at the surface of the agar slants. The slants will be incubated for at least 18 to 24 hours at 37°C, and the results will be recorded afterwards. 4. Oxidation-Fermentation Test (OF Test) In OF slants, a loopful of culture will be stabbed halfway to the bottom of the…

It is shown in Table 2, that each bacteria and fungi had different agar cultures. Each plate differed in its number and diversity of the colonies. For instance, the soil sample consists of a variety of punctiform that prove bacteria grows better in moist, warm environments, as the soil is underground. Furthermore, the other samples…

As a negative control, the test strain should be inoculated alone. Inoculate in the centre of the plate (V8 juice agar) or Eucalyptus tree and incubate at 25oC in the dark for two weeks. The plate or bark is observed periodically for the sexual development i.e perfect state. Mating can be observed by seeing the dense filament growth surrounding the colony and is confimed by observing dikaryotic…

Autoclave the tubes Preparation of Inoculums and its Standardization: 1) Take loop full of culture from working slant of respective organism and streak it on the respective agar medium. The ATCC microbial culture strains were revived and sub-cultured on the slants of Soybean casein digest agar and Sabouraud dextrose agar for the growth of bacteria…