Agar plate

Then, a uniform cultivation was done by sterile cotton swabs soaked in suspension on the surface of Mueller-Hinton agar (Merck, Germany). Then, the disc containing the extract with concentrations of 500 μg/ml, 250 μg/ml and 125 μg/ml, was removed with sterile forceps and placed in a certain distance from each other, from the edge of the plates, on the surface of Mueller. The plates in an upside down state were incubated at 37 °C for 24 hours, and the diameter of inhibition zone around the disc…

microbiota or other contaminants via plate streaking on four selective and differential media namely: Mannitol salt agar (MSA) to inhibit the growth of most organisms and differentiate possible pathogenic staphylococci, Phenylethyl alcohol (PEA) agar to grow gram-positive organisms, Eosin methylene blue (EMB) agar to cultivate gram-negative bacteria and isolate fecal coliforms, and Nutrient agar for a general purpose to support the growth of the unknowns. All plates were incubated at 35˚C for…

Introduction: Microbiology is the study of all living microorganisms like bacteria, fungi, protozoans…etc, and non-living microorganisms like viruses, viroids and prions. The significance of identifying an unknown microorganism is first to understand what type of organism it is and what structures it has to help figure out their potential benefit, danger or even neutrality to humans. Physicians benefit from this by identifying what their patients are suffering from and how to treat them. Also…

the different types of motility arrangements were presented and why true motility helped microorganisms in their environment. Motility agar was used in this experiment and a needle saturated with the unknown microorganism was inserted straight into the agar. The following week it was found that there was no bacterial growth outside of the stab line in the agar which indicates the unknown as being nonmotile. The unknown microorganisms morphology and the arrangement were identified after…

continuing on ahead doing the catalase test she had recommended that I do a mannitol salt agar test or a Blood agar test for my final test, which would help determine between Streptococcus pneumoniae, Staphylococcus epidermidis. I decided to go along with the mannitol salt agar method and again with my inoculated loop I grabbed some of my unknown #15 and I had applied it to aseptic technique to the agar plate. While waiting for my results to appear, this test will show us whether or not the…

Motility Test. Due to unknown 10 being gram positive, coccus shaped, and a non-sporeformer many bacteria were eliminated. One test that was run to help in differentiating the bacteria is the motility test. The motility test is preformed by taking soft agar, and heavily inoculating it an inoculating needle. The needle will create a line where the bacteria will diffuse out of it is motile. However, unknown 10 did not exhibit any diffusion, classifying it as a non-motile…

have on a culture of yeast cells in two different agars, testing the rate of mutations and cell death that occurs. The overall purpose of the experiment is to connect the…

Violet red bile agar is a selective medium used to identify lactose-fermenting coliforms. This medium is typically used to test dairy products and water. The medium contains lactose which acts as a carbohydrate source, bile salts and crystal violet that act as an inhibitory agent towards gram-positive microorganisms and neutral red is added to serve as a pH indicator, microorganisms lower the pH which results in the colonies turning red. Some of the organisms that grow on this medium are:…

If there is coliform in the water sample, the color will change from purple to yellow, indicating that the coliform bacteria ferments lactose. The next test that is used to analyze is the EMB agar test, a differential and selective media designed to distinguish the colonies of different microbes and encourage the growth of the desired microbes, while inhibiting the growth of unwanted microbes. Due the media’s composition, it can distinguish…

results. 5-bromo-4-chloro-3-idolyl-b-D-galactoside (X-Gal) is used on the agar plates that the transformed cells are placed on because when digested by beta-galactosidase it produces a blue component part that is visual to the naked eye. The X-Gal can only be digested if the gene encoding beta-galactosidase is fully functional meaning if there is blue then the transformation was successful. The other agent on the some of the agar plates is Ampicillin, which is an antibiotic that kills E. Coli.…