Agar plate

Then, we looked at the Tributyrin agar and looked for a clearing/halo around the bacteria. After this procedure, we inoculated three urea broths and after storing it at our optimal temperature for 48 hours we brought it out and examined them for color change. After these tests had been…

BACTERIAL TRANSFORMATION By Derek Tang BIO 281 Lab section 80243 Abstract: The objective of this experiment was to observe the results of bacterial transformation in various growth conditions and determine how successful the altered organism is after the transformation. The organism used was E. Coli because singular cell organisms can have a new gene more easily inserted than multi-cellular organisms. The gene which was inserted is pGLO that carried the gene encoding GFP and a gene for…

case three test are essential to identify the unknown #63 and they are MAC agar test, BCP (lactose) broth and SIM deep medium/slant. Methods such as streak plate technique, aseptic technique, and proper handling of the microbe are necessary to apply when conducting these biochemical test. Contamination during the unknown lab project shows carelessness and may put patient in jeopardy due to incorrect result(80). The MAC agar plate that was stored in the incubator at 37°C for about 48 hours until…

2012) Some errors were that the music playing could have been not exactly the same, the agar that was poured at the beginning of the experiment could have been poured unequally, and the bacteria could have been distributed unequally. Some improvements that could have been done to the experiment were a longer growing time, having more varieties to the music, and measuring the amount of agar into each petri plates. Both table one and figure one have shown that no music grows the most bacteria,…

observed on four agar plates containing different combinations of LB broth, ampicillin, and arabinose. The second indicator was fluorescence, which was observed under UV light. The presence of fluorescence indicated that the bacteria had taken up and expressed the pGLO plasmid, which contained the GFP gene that produces green fluorescent protein. The four plates used in the experiment were the +pGLO LB/AMP/ARA plate, the -pGLO LB/AMP plate, the +pGLO LB/AMP plate, and the -pGLO LB plate. The…

colonies of E. coli mixed either with a pGLO plasmid or without a pGLO plasmid were placed on four Luria-Bertani (LB) agar nutrient plates. One plate had only LB, two had both LB and ampicillin, and one had LB, ampicillin and arabinose sugar. We hypothesized for this study that the E. coli mixed with the pGLO plasmid would take up the plasmid and would then thrive on the agar plates with ampicillin because the pGLO plasmid contains a gene for ampicillin resistance. It also follows then that the…

the best tests and mediums to determine the unknown bacteria.This medium are use to test the metabolic characteristics of the unknown bacteria. In order to identify the unknown bacteria from other bacterias the following medium was used: MSA plate, SIM plate, nitrate reduction test Nutrient gelatin and Urease broth. For Metabolic tests, only Catalase test were performed on the unknown bacteria. These tests were best use gram negative bacteria to indicated what the unknown microbe is. For the…

experimenting will need goggles, gloves, and an apron. To do the project, you will need nine glass petri dishes (make sure you get them sterile), agar, beef broth, sterile cotton swabs, three slices of bologna, three slices of ham, and three slices of turkey. Experimental errors would be breathing on the plate of agar, amount of agar, and water on the plate of agar. In conclusion, the results did not support the hypothesis. The bologna grew an average of 15 total colonies and an average of two…

virus that virus is called a bacteriophage. After a bacteriophage infects a bacteria it can create two different phases, the lytic or lysogenic cycles. During a lytic stage the bacteriophage causes death to the bacteria. When this happens on an agar plate covered in bacteria it creates a clear spot known as a plaque. Each plaque is created by one virus so from the number of plaques present it is possible to govern how many viruses are in a culture. During this lab this Plaque Counting Assay was…

INTRODUCTION Being able to identify an unknown specimen in microbiology is vital in the laboratory. The goal of this experiment was to isolate bacteria on a nutrient agar (NA) plate to obtain pure colonies of the unknown specimen. This study was done by applying the methods learned in Professor Sprenkles microbiology lab in determining the unknown bacterial growth present with use of pure culture colony morphology, cell morphology and arrangement, and Gram reaction. MATERIALS AND METHODS The…