Agar plate

First the unknown tube was isolated. This was completed by taking an inoculating loop and spreading the unknown out on to a Nutrient Agar plate. This was done using the streak plate method, which is described in the lab manual. The bacterium were incubated and grown at 48 hours. Upon observation the isolation plate showed growth of two different colonies. After the morphology was observed and recorded, a Gram stain was performed on each of the two unknowns, this procedure is found in the…

streak agar plates to culture any microbes collected in the swabbing process. The microbe-exposed plates were then sealed and incubated at twenty-eight degrees Celsius for one week. Without environmental disturbances or nutritional competition, species growth was only restricted…

inhibition to lowest. These predictions that was base on previous experiments from (Mcbride et al 2007) and (Wendelbo et al., 2003) were falsified. There is however limitation to my experiment because of the small sample size that consisted only of five agar trays. If more trials were done, we may get results closer to the sources. Since erythromycin is being supported by my data to be a more effective antibiotic, doctors can prescribe this antibiotic to patients with…

Ex: In the agar diffusion method, single bacterial species is uniformly swabbed onto a nutrient agar plate. Control agent of different concentrations is placed on paper disks. These paper discs are kept on the surface of the agar. Control agent diffuses from the paper disk into the surrounding agar. Based on the zones of inhibition size around the disks, it can be evident that an effective agent will inhibit…

majority of the plates had growth in them. The agar plate that had the antibiotics, but not the plasmid had no growth because the bacteria was not introduced to the plasmid that had the antibiotic resistant gene.The LB/amp had growth to it because it was not introduced to any new molecules or chemicals. The po The other plates had growth because they was introduced to the antibiotic resistant gene that made the bacteria able to grow. The positive control of the experiment was the LB plate,…

and can grow colonies on the agar plates. For example, in the LB (- DNA) shown large number of white because contain negative DNA but it is present LB. In the LB/Amp (pGLO negative) shown any growth colony because it is presents Ampicillin that break the bacterial cells. In the LB/Amp (pGLO positive), shown growth colony regardless this has Ampicillin. The reason in this case, it is because in the positive DNA has pGLO that can grow bacteria colony on the agar plate.…

Abstract Many number of medicines, like growth hormones and insulin, are a possibility because of techniques like transformation of genetically engineered bacteria. Although, manipulating bacterial gene expression has many great implications, it can also be quite harmful. By manipulating gene expression of bacteria, such as Escherichia coli, scientists now have the capacity to help bacteria survive changes in their environment and provide an evolutionary advantage. The present study was…

celled. I also put the bacteria on a streak plate. On the second day, I did another gram stain and the bacteria were again pink and bacillus or rod shaped. I also observed the bacteria on a nutrient agar plate and noticed that the bacteria were light white almost clear color bacteria. I took the bacteria that were…

The Bile Esculin test was performed on a Bile Esculin Agar slant by inoculating the slant in a zig-zag pattern and was stabbed using an inoculating needle. The slant was then incubated at 37°C for 48 hours. After incubation, the slant was taken out and observed for any change. The Bile Esculin Agar used in the test was used as a selective and differential medium. The bile that was in the medium was selective for streptococci…

activity of the drug Tal Sindoor can be tested in 2 methods i.e. Gradient plate technique and Kirby-bauer method. Tal Sindoor has an effective anti-microbial activity against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Streptococcus mutans and Candida albicans. But K. pneumoniae and A. Baumannii are resistant to Tal Sindoor like they are with other…