Agar plate

Striker Loops and Needles Distilled Water Crystal Violet Alcohol Safranin Iodine Methylene Blue Hydrogen Peroxide Reagent A (sulfanilic acid) Reagent B (dimethyl-alpha-napthylamine) Kovacs Reagent EMb plate Blood plate MSA plate Starch plate Litmus milk tube Nutrient agar Slant…

Streptococcus pneumoniae is placed in a blood agar plate, Penicillin will prevent the bacteria’s growth, while Tetracycline and Kanamycin will have no effect. Therefore, if Streptococcus pneumonia is given one week to grow, then there will be a 1 mm zone of inhibition around Penicillin and a 0 mm zone of inhibition around Tetracycline and Kanamycin. Materials and Methods Prior to beginning, the work stations were cleaned and gloves were placed on. Blood agar plates were labeled and two…

when testing for the sensitivity of microorganisms using antimicrobial agents. One of the biggest is the range of pH, which should be between 7.2-7.4 and have a thickness of 4mm. Another is the turbidity and the amount of organism placed onto the agar plate. All of these can have an adverse effect on the…

mixture of bleach and water and gloves and goggles should be worn during this process. Procedure Prepare Agar Plates by heating a mixture of 1 Liter purified water and 23 grams Nutrient Agar powder for 15 minutes on a hot plate at 121ºC while stirring consistently. Once the mixture is clear, pour it into the petri dishes one at a time by just barely covering the surface of the plate. Set the plates to the side to settle Tape the meter stick to a counter to measure the drop height of 1.0…

This explains why there were no colonies in the SD plate which had no radiation exposure time. Small amounts of radiation would produce some mutations, but not so many that mutations are likely to occur in multiple locations in the genome and have lethal effects. Therefore, the number of colonies that were present decreased with increasing radiation exposure time. The SD plates which spent the longest under the lamp likely had too many mutations overall and one or…

Mannitol Salt Agar and Phenylethanol Agar are selective media that were used to isolate M. luteus from the environmental samples. Sterile cotton swabs were used to obtain the samples from the skin, arms and dust through wiping the surface of the sample source. A handful of backyard soil was previously diluted and then 2mL of this diluted soil was transferred to a sterile test tube containing 9mL water using a pipette. Four MSA and four PEA plates were used for isolation. PEA plate number 1 was…

identify the pathogen causing disease. The goal of this lab was to be able to identify the unknown pathogen, match the patient to the bacterium, and being able to identify the disease it causes. At the beginning of class, the TA handed each student an agar plate containing some unknown bacteria, each one was numbered. Mine was number 14. The teacher then asked the students to use the techniques used in class (sterilize the inoculated loop, get a small amount of the unknown bacteria, incubate) to…

then carefully streak on quadrant one of the plate. Afterwards, the loop was sterilized by the flame and was allow to cool for thirty seconds so the next streak can be applied. The same concept was then applied for the different metabolic test. It is important to practice aseptic techniques to prevent contamination of results (3). A broth of mixed unknown bacteria was given and labeled…

Before inculcating the TSA plate, the surface of the agar and sides of the plate were carefully examined for any visible contamination. A bacterial culture was obtained and a sterile non-toxic swab was gently inserted inside the tube rotating around the culture. The swab was removed by carefully pressing the inside wall of the tube, removing any excess inoculum from the swab. The plate was inoculated by streaking the swab over the entire agar surface in three directions. The plate was rotated…

microorganism can be sampled in a dry environment and be identified through observing unique characteristics that would make the microbe be considered either a mold, yeast or bacterium. Using a nutrient agar plate and cotton-tipped swabs for collecting the microbes, colony growths can be observed in the plate and inoculated onto a slide for further microscopic observation. The hypothesis was not entirely rejected as certain unique traits of the microbe collected was identified closely to a…