Agar plate

test how the ecology of life works, we measured and reviewed the growth of bacteria after washing one’s hands at different intervals of time. After washing our hands four times in 30 second intervals, we wiped a finger on a different segment of an Agar plate where our bacteria would have a strong environment to grow and develop. To be sure that each individual whose’ hands would be tested had a source of bacteria on them, we had the freedom of choosing any object in or around the school before…

a pure culture from the mixture onto a solid Trypticase soy agar (TSA) media. Sterile technique was done by flaming the loop until it turned red to ensure that there were no current bacteria on the loop avoiding contamination followed by rapidly flaming the neck of the test tube to prevent the entry and contamination of unwanted microbes. After inoculating the loop substance from the test tube labeled ‘I’ was removed. The streak plate…

compare the effect of using soap (antibacterial and non-antibacterial) and no soap on the growth of Escherichia coli (E.Coli). Hypothesis: If anti-bacterial and non-antibacterial soaps are added to a standard agar media before the E.Coli was introduced and incubated, then the portion of the plate containing the antibacterial soap will have the least amount of bacterial growth because the antibacterial soap contains a higher percentage of bacteria-killing chemicals. Theory: E.Coli is a…

for at least 24 hours. This is done to allow the agar plate to get used to the environment and also to allow a zone of inhibition to be seen on the agar plate if the antibiotic is…

place three filter paper circles) into each baby food jar (#1 through #5). Let them soak until you need them in step 10 3. Lay out five of the nutrient-agar prepared media plates. Place the plates upside down on the clean counter or table and use the permanent marker to label the back of each one, #1 through #5. 4. After labeling them, flip the plates over so that the lid is on top. 5. Take off your gloves and put on a new pair of disposable gloves. 6. Sterilize the tweezers by dipping them into…

placed in agars with ampicillin and without ampicillin. The agars are later inverted and incubated at 37ºC. After a certain time the results are analyzed. Two alternative hypotheses made in this experiment are: 1) the presence of ampicillin will have an effect on bacteria with plasmid 2)there will be a difference in bacterial growth between plasmid lux and puc18. Some predictions are: 1)The agar plate with ampicillin and bacteria without plasmid is going to show no growth. 2)The agar plate with…

The results indicated in Figure 9.1, represent how in the six agar plates obtained, three of them had ampicillin, while the other three had no ampicillin.…

first plate (LB agar with -pGlo E. coli) and the second plate (LB+Amp with -pGlo E. coli). There were two control groups in this lab because both a positive and a negative control were needed to be sure that the E. coli were able to grow, and that the ampicillin antibiotic were not expired or faulty. The first plate (LB agar with -pGlo E. coli) was the positive control group because the expected results were that the E. coli would grow, there for making the results positive. The second plate…

It is hypothesized that Bacteria X will create only narrow spectrum antibiotics. This will be tested by adding the bacteria to a small area on two agar plates – one plate with a gram negative ESKAPE pathogen and the other with a gram positive ESKAPE pathogen. If the bacteria grows on both plates and has a zone of clearance on both plates then it is likely that the bacteria is effective in broad spectrum against both gram negative and gram positive bacteria; this would be the most effective…

80 grams of agar nutrient powder. Then add and dissolve agar nutrient powder in conical flask containing 600 ml of the deionise water. Both these flask are autoclave and sterilise at temperature of 121 ℃ about 20 minutes at 1 atmosphere pressure. After the autoclave is done for these flask containing the agar nutrient, it will be let cool to 50 ℃. The mixture is swirl throughly to mix agar and nutrients. Then the nutrient agar are pour and fill 2/3 full roughly 25-35 ml per petri plate and…