Agar plate

to correctly perform this lab, plasmids, agar plates, Escherichia coli colonies, as well as incubation periods were used. The data that was collected was completely opposite than what the actual outcome should have been. Yes there was growth in the agar plates however there was not as much growth as predicted. Another problem that arose was the fact that the pGLO did not attach to any Escherichia coli DNA, so when under a UV light, none of the agar plates glowed, even the ones that were supposed…

bacteria is by using agars and staining. The agars that are used in this lab are eosin methylene blue agar (EMB), mannitol agar, and nutrient agar. Mannitol agar is used for identifying bacteria that fall under the Staphylococcus genus (Beaver, L et al. Mannitol Salt Agar). This agar has a very high concentration of salt which is a good environment for the growth of Staphylococcus bacteria only (Beaver, L et al. Mannitol Salt Agar). The next agar that is used is EMB. EMB is a good agar for…

able to form on the agar plates. All the experimental plates had colonies including the agar plate of the enzyme of DNAse. Which simply contradicts the transformation principle, this effect could arose by systematic errors that have occurred during the experiment, that is, the enzyme DNAse was inactive therefore could not interfere with the growth of the bacterial cells and/ or could be result of not preparing the experiment in sterile conditions. However, goal standard plates were set up for…

Proper aseptic technique was used throughout the semester and followed during all biochemical tests preformed, which are described throughout the paper beginning with a streak plate. A streak plate was completed first in order to isolate colonies of the unknown bacteria. This was done by pouring nutrient agar into a plate, waiting for it to solidify,…

The first test was hemolytic reaction; obtaining a blood agar plate, the unknown was swabbed onto the medium and incubated. A Bacitracin test was performed by also acquiring blood agar and swabbing the unknown bacteria onto the plate. A filter paper disk containing 0.04 units of bacitracin was then placed in the middle of the streak, and the plate was incubated at 37C. The next test performed was the Bile Esculin Test. A bile esculin agar slant was inoculated with the unknown, and incubated at…

were a skim milk agar plate and the pure culture of Neisseria sicca. For the catalase test, 3% H2O2 solution dropped onto the agar plate using pipette. A few EA & JB 4 moments of waiting was observed and a bubbling appearance occurred indicating the presence of catalase. For the oxidase test, an ampule of reagent was added to the streak plate of…

We were given three agar plates by our TA, each agar plate containing a nutrient broth. We labeled each agar plate with whatever content we were going to put inside of it. We worked with the control so our three labels were “LB^NP, LB^AmpC, and LB^C”. LB^NP means the broth with no plasmid. LB^AmpC means the broth with…

series of tests was done with Microbe #1R for identification. A Gram stain was done it reveal a gram positive cocci result. A streak plate was completed revealing that the microbe was round/filamentous, convex, erose, granular, yellow, and opaque. The next test was the Tryptic Soy Agar plate for color. Followed by the oxidase test and last was the Mannitol Salt Agar test. Thus microbe #1R was Sporosarcina ureae (Brady, Personal Communication). Procedure of the Gram stain Purpose:…

streak plates and TSA agar, the identity was determined from a Gram-stain of a single, isolated colony; this was confirmed by isolation of that pure colony and viewing the pure sample in a Gram-stain under a microscope yielding a blue colored cocci morphology with no Gram-negative contamination. This was followed by Gram-negative isolation by a Gram-stain showing a myriad of Gram-negative bacillus rods, with no presence of Gram-positive. Further tests…

preheated bath, where the cell is heat shocked, allowing the plasmids ' to enter the cell at a rapid pace, once the cell expands. After the tubes are incubated, they were transferred to six agar plates. There were two types of agar plates, one included the plasmid lux and the control plasmid pUC18. Every agar plate was labeled independently…