Agar plate

Streptomyces and Antibiosis From our group, Group 2, nutrient agar plates inoculated with E. Coli, (Images 1.1,1.2), (Images 2.1,2.2), (Images 3.1,3.2) no antibiotic activity was detected. This could be a result of the putative colonies chosen, not being Streptomyces. All selected colonies were white, or pigmented rink, raised and rough. One (1) and three (3) were inoculated with the putative Streptomyces colonies. Plate 2 was inoculated with the control Streptomyces colony. Although it was…

bottle of melted agar. Remove the cap and pass the neck through the Bunsen burner flame 2-3 times. Pour the contents into the Petri dishes carefully. Replace the lid as quickly as possible. Flame the neck again and replace the cap on the bottle. Keeping the Petri dish flat on the bench, mix the agar thoroughly by gently swirling the plate as directed by your teacher. Avoid splashing the agar on to the lid or over the edge of the dish. Allow the agar to set. This is known as a pour plate. Use a…

amount of the bacteria to the droplet of KOH. Stir it together. Raise and lower the toothpick off the slide to observe the results. Obligately aerobic organisms will grow on top but not in the stab. Motility Agar: Obtain a motility agar tube. Using an inoculation pick stab 2/3 of the way into the agar from your unknown stock culture as straight as possible. Incubate for up to 72 hours. Then observe your culture by holding it up to a light source. Results depend on visual stance, if the growth…

as a Nutrient agar plate, MacConkey’s agar plate, Gram staining, Oxidase test, UTI ID Membrane, and Eosin Ethylene Blue Agar. Materials: Testing Unknown Bacteria #8 Materials: 1. Nutrient Agar Plate (NA) 9. Hydrogen Peroxide - 2 g/ml. MacConkey’s Agar Plate (MAC) 10. UTI filter 3. Eosin Methylene Blue Agar Plate (EMB) 11. Petri plate 4. Unknown Bacteria #8 - 12. Forceps 5. Bunsen Burner - 13" Timer 6. Inoculating loop 7. Gloves 8. Oxidase test strip Methods: 1. Nutrient Agar plate: Taking…

short chains and some single cells. After noting these observations, I inoculated a blood agar plate and a Phenylethyl Alcohol agar plate with the unknown #260 mixed culture broth. The blood agar plate, shown in Figure 2, was inoculated using…

wanted to prove the microbiologist that on this planet has microbes everywhere. To understand more about ubiquity experiment, an important terms need to be explained. Agar is gelatinous- complex polysaccharide obtained from red algae. An agar plate is a Petri dish that has an agar and nutrients used to culture microorganisms. Also, agar can be expand by adding other nutrients such as glucose and other…

swab of any surface, including the human body, will yield many different types of microorganisms. Once grown on a nutrient-rich medium, the identification of these microorganisms can be made by isolating pure colonies and performing and using various plates and broth and recording its characteristics. It is important to understand and characterize each microorganism, behavior, reaction, and appearance for future purposes. In this experiment, the identification of two unknown bacteria was…

in this lab are a sterilized Agar plate, sterilized, 1 P20 Micropipette. P20 micropipette tips, 4.5 milliliters of Top Ager, 0.5 milliliters of M. smegmatis, 2 serological pipets, and 5.0 milliliters of Phage buffer. The protocol is to create agreed on the bottom of the Agar plate to look like a checkerboard. Flip it back over with a cover face in the ceiling. Extract 4.5 milliliters of Top Agar and place it into the 0.5 milliliters of M.smegmatis. Release the Top Agar. Withdrawal of the mix…

The same process was repeated for the next three hand cleaners and sanitiser, into three separate agar plates. The tweezers were washed and sterilised in between each agar plate The labelled plates were placed in a designated area, upside down, over the period of three…

culture, and conducted a variety of biochemical tests. First, I inoculated a blood agar plate and a MacConkey (MAC) agar plate with the unknown #260 mixed culture broth. The blood agar plate, shown in Figure 1, was inoculated using the quadrant streaking method and then incubated for 24 hours at 35 °C. In the third and fourth quadrants, two different bacterial cultures were present. The bacteria that covered most of the plate were light gray, circular, convex, entire colonies in a punctiform…