Adsorption

preconditioned cartridge. After loading, the vacuum was still applied to the cartridges for 5 min in order to remove the residual solvent. 1 mL of ethanol/water (8/2, v/v) were added to the cartridges to eliminate molecules retained by physical adsorption by the polymer. Eluting step was performed using 2 mL of methanol/acetic acid (9/1, v/v) mixture solution. The elution fractions were dried under a gentle nitrogen stream. The residue was dissolved with 1 mL of methanol and filtered through a 0…

when no growth is taking place. Hershey and Chase then, proceeding in the direction of infecting cells, used the blender experiment to suspend these infected cells. From this test, they determine that DNA accesses the cell directly after the phage adsorption. Hershey and Chase suggest that DNA plays a role in the forming of phage growth (whereas they state that the protein has no responsibility for the growth of the phage), since the temperature changes do not release any of the phage DNA from…

5. Protein purification There are many thousand kinds of proteins with different properties and functions in a cell. Proteins have been purified in active form on the basis of such characteristics as solubility, size, charge and specific binding affinity. Normally, protein mixtures are subjected to a series of separations, each based on a different property to yield a pure protein. 5.1 Separation based on solubility (Scopes, 1994) The protein solubility in various solvents depended on the…

The pharmacokinetics of morphine and codeine in human plasma and urine after oral administration of a papaveris pericarpium containing traditional Chinese medicine The opiate morphine (MOR) and codeine (COD) are found in natural poppy products of papaveris pericarpium, which is the principal active component in many traditional Chinese medicines for treating cough. Despite its long history of use, little is known about the pharmacokinetics (PKs) of papaveris pericarpium, which are important…

feed stuffs of oligosaccharides and polysaccharides, including dietary fibre can be referred to as prebiotics. (Lindberg, 2014). Additionally, for a feed ingredient to be classified as a prebiotic it should be able to resist digestion, absorption, adsorption and should be fermentable in the host gut by the microbiota which are situated in the gastro intestinal system which will stimulate the growth and activity of one or more bacteria in the system .Similarly dietary fiber can play an important…

Electrical and mechanical functions integrated in the nanoscale range led to the development of the new age devices, Nanoelectromechanical systems (NEMS). These devices because of their size, they come with properties such as reduced mass, high quality factor (Q factor) and very high fundamental resonance frequencies. These qualities of NEMS makes them desirable for numerable applications such as components of signal processing devices, actuators and high speed sensors. NEMS can be used to…

without decomposition ("Gas Chromatography", 2016). This technique involves using a gas as the mobile phase. There are two types of gas chromatography: • Gas-solid chromatography – separates substances on the basis of their different strengths of adsorption on the solid • Gas-liquid chromatography – separates substances in the basis of different solubilities in the stationary phase How does it work? In a gas chromatograph the sample being analysed is inserted with a syringe into a heated flowing…

Bullseye Glass is a local glass manufacturing company in Portland, Oregon. In May 2016 Oregon Department of Environmental Quality (DEQ) reported that on May 9th and 10th the company had violated the 24-hour benchmark and released 4x higher amounts of lead that allowed and that the Bullseye Glass company was the source. (OPB, 2016.) The company responded by claiming that DEQ is attempting to close the company down. The issue had been centrally over the production of a “white opal” glass, which…

the precipitate under these conditions for 12 hr gives the highest CO conversion. During the drying period of the solution retained by the porous support may migrate by capillary flow and diffusion and the solute redistributed by desorption and re-adsorption. When the solvent evaporates, precipitation of solute happens as the solution becomes supersaturated and brings crystallization of the precursors in the pores and outer surface of the…

The schematic diagram presented in Figure 1.1 illustrates the experimental rig used for the kinetic analysis of reactive flash volatilization (RFV) of acetol and guaiacol. The flow rates of oxygen and nitrogen gases were regulated using mass flow controllers (the type and serial number). Water, on the other hand, was delivered to an evaporator by a high-performance liquid chromatography pump (Agilent) at rates between 0.06 to 0.17 ml/min. The evaporator set at a temperature of 180C allowed to…