it has been argued that proteins can physically adsorb onto MWCNTs in the EDC/NHS-mediated immobilization [15]. Therefore, it was necessary to investigate whether the observed GOx immobilization occurred through covalent attachment or physical adsorption. The inclusion of Tween-20 in the post-reaction washes or its addition to the reaction mixture is known to effectively remove any physically adsorbed proteins during immobilization [42]. Accordingly, the effect of Tween-20 was examined for…
Introduction: LC-MS is used for the bioanalysis of small molecules drugs, which has molecular mass of less than 800 Da. Now a days, it is also frequently used for the bioanalytical determination of biopharmaceuticals.1 In pharmaceutical R & D, it takes a year to develop a new marketing drug identified from thousands of new compounds. The drug discovery is divided into early and late phase, during which pharmacokinetics study or bioanalysis of a drug is done. Once, the bioanalysis of a drug is…
One of the most widely studied mitigation options for reducing concentration of CO2 in atmosphere involves injecting it into the ocean at great depths, where the gaseous CO2 reacts to form carbonic acid (H2CO3) as shown in equation (2.1). This equation shows various forms of CO2 as it is dissolved in the water (brine). Depending on the pH of the solution, either forms of CO2 may be observed (CO2 (aq), 〖"HCO" 〗_"3" ^"-" , 〖"CO" 〗_"3" ^"2-" ). There are two ways for storing CO2 in ocean, either…
In this research, mesoporous material KIT-6 silica with high surface area (924.5 m2/g) and pore diameter (9.71 nm) was successfully synthesized using rice husk ash as a silica source. According to the SAXS analysis and TEM micrographs, the silicate structure of as-prepared KIT-was not distrupted by the functionalization and activation treatments with 3-APTES and glutaraldehyde, but showed a significant decrease in pore diameter and total pore volume as the compound was covalently bonded to the…
diagram [20]. The alloy 1 presents OCP value of about -1.30 V whereas that of Alloy 2 was -1.44 V, which are more negative than that of Al in the same medium. The presence of tin and gallium in aluminum matrix as alloying elements increases the adsorption of Cl- ions at the values of negative potential which leads to the alloy dissolution [10]. The surface morphologies of Al, Alloy 1 and Alloy 2 after immersion in 2 M NaCl solution for 1 h were obtained by scanning electron microscopy (SEM) (Fig…
The peak at around 2200 cm-1 attributes to N2O absorbed on Cu[13, 27]. It could be found that there is a competitive adsorption between O2 and N2O. But the competitive adsorption seems too slight to account for the obvious decrease of activity. As discussed above, I proposed that the regeneration of Cu+ is the rate-determining step, and O2 may affect the reaction by inhibiting the regeneration step…
discussed. The chlorine reacted with organic matter in the water forming a common class of disinfection byproducts, trihalomethanes (THMs), which are tasteless and odorless known carcinogens (1999). THMs can be taken into the body via drinking and adsorption through the skin (Hood, 2005). Therefore, not only is drinking the water a concern, but now, the water is damaging just to be in contact with all before Pb introduction. Concentrations of these trihalomethanes are unknown and generally…
SAMPLE PREPARATION: Wilcox shale and Pottsville shale samples are tested with the indentation method in this experiment. Both samples are quartz rich and silty shales which contain about 45 to 50% of quartz, 40 to 45% of clays and about 5% of plagioclase. Wilcox shale sample is from Louisiana and formed in upper Cretaceous while Pottsville shale samples are from Alabama and formed in upper Pennsylvanian. These two type of shale rocks are chosen because they are “local” rocks which was abundant…
In this research paper, the functional relationship between protein named Albert and Alice will be prove using two advance techniques. Most of the molecular processes within the cell are carried out by protein-protein interaction (PPI), diseases such as cancer, Alzheimer’s disease can caused by abnormal PPI. As prior knowledge has advocate that this two proteins may have similar cellular pathway, therefore understanding if they have PPI becomes vital. There will be two methods described in this…
1. INTRODUCTION 1.1 What is biogas? Nowadays hydrocarbons are one of the biggest energy bases of our society, but it is not renewable energy, and once they are exhausted they cannot be replenished. (but as it is not a renewable energy once they are exhausted they cannot be replenished) Therefore, scientists from around the world have researched the use of other energy resources to continue moving the world, such as solar and nuclear energy, which are two main areas of research. However, there…