1ml per sample. The samples were incubated at 4⁰C overnight. The following day, the samples were washed with DPBS at 4⁰C and then washed with 2ml 1 perm buffer twice. Anti-mouse Foxp3- PE Ab was added in a 1:100 dilution for 30 mins at 4⁰C. The samples were washed with perm buffer and resuspend in DPBS for flow cytometry analysis. Table 2.4 Antibody panel for SHPS-1 Phenotyping Panel PerCP FITC APC PE PB DC 1 CD45 CD11b CD11c CD103 B220 DC 2 CD4 CD11b CD11c CD8 B220 Treg CD3 CD25 FoxP3 CD4…
Did you know that whenever you smell something, it triggers your senses, and the brain? There are five senses, and one is smell. The brain is a important organ in our body, it tells our body what to do, and what to not do. The person who studied the senses was Linda B. Buck, and the people who studied the brain was the Egyptians. Your senses let you know what is going on around you. The brain is the control center of the body. All information we have about prehistoric cultures comes from…
of nicotinic acetylcholine receptors (nAChRs) early on in the progression of the disease (Okada et…
Enzymology and Catalytic Mechanism Carbohydrate Metabolism, Adenosine Triphosphate Western Governors University ENZYMES • Are proteins that act as catalysts, and carry out chemical reactions.…
Determining Enzymes that Result to Mutation on the three Stages of Cellular Respiration Mary A Enriquez University of the Pacific November 11, 2014 2 Green Dr. Geoffrey Lin-Cereghino Abstract: The purpose of this lab is to determine which type of food source could possibly help strains grow on both permissive and restrictive temperature. Based on all the chemicals accumulating, we can determine the enzymes that are used to inhibit the three stages of cellular respiration. Introduction:…
Rafaela Hajdini Bio 104- 55 November 11th 2014 Fermentation Lab Report Abstract: Introduction: Cellular respiration is a reaction that the majority of living organisms partake in to get adenosine triphosphate (ATP), by obtaining chemical energy, to synthesize in three different phases; glycolysis, citric acid cycle, and the electron transport chain. Both glycolysis and the citric acid cycle are anaerobic pathways which means that oxygen is not need to form energy. However the…
Assignment 1: Metabolism Gone Wrong: Galactosemia Course: Biochem 3D03 TA: Rabia Mateen Name: Kei Cheng Mak ID: 3122260 Date: October 15th, 2014 Introduction Metabolism consists of biochemical reactions catalyzing the conversion of complex macronutrients into usable energy by various enzymes. Energy in food is extracted from three macronutrients - carbohydrate, protein and fat. As food passes through the digestive tract, various enzymes and chemicals in the body break food particles into…
Unfavorable has a negative connotation, it conjures up thoughts of ‘bad” and “non-useful” processes, however as we have learned through the case studies of muscle contraction and photosynthesis, this is simply a misconception. For in the natural world a favorable or unfavorable reaction is not a single entity but is rather part of a coupled reaction that uses the two in tandem to continue the function of essential processes. This being said, and connotation aside, an unfavorable reaction is one…
The human body requires energy to fuel growth, development, and repair in order to sustain proper functioning and remain alive. For humans, this fuel source is food. For example, as Koletzko, Goulet, et. al. researched in infants, they found that infants born with gastroenterology diseases or disorders were unable to harvest energy from food they consumed and therefore suffered severe growth stunts and defects. The relationship between food and calories can now be established because food…
DNP’s a Weight Loss Miracle? Or a Devastating Chain Link of Events? DNP’s a Weight Loss Miracle? Or a Devastating Chain Link of Events? By: Ashveen Sharma As with the many possible weight loss and fat loss options, theories, practices, and scientific reasoning the chances and possibilities of achieving weight loss are endless. From basic strategies such as cardio, and exercising to more advanced such as steroids and insulin injection. However, not all are created equally; one drug…