Acrylamide

the SDS-PAGE gel yielded expect bands and the approximate molecular weight of wild type alkaline phosphatase and maltose binding protein-alkaline phosphatase is 49 kDa and 95 kDa, respectively. Additionally, the concentrations of MBP-AP Elution 1 protein and WT-AP Stage 4 are 0.278 mg/mL and 0.582 mg/mL, respectively. Introduction To determine the purity of a protein, a sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is often utilized. It requires two basic components: an electrophoresis chamber, which is a gel box that contains the gel, and a power supply that provides constant and suitable current. To prepare the polyacrylamide gel, free radical polymerization of acrylamide is performed by reacting acrylamide with the cross-linking agent N,N’-methylene-bis-acrylamide. The reaction is controlled by an initiator-catalyst system, ammonium persulfate (APS)–N,N,N’,N’-tetramethylethylenediamine (TEMED). Sometimes, sodium dodecyl sulfate is added to the gel mixture as a disulfide bond reducing agent, which helps denature the proteins. Once the polymerization process is complete, the liquid mixture will become a gel. In the case of AP proteins, a slab gel electrophoresis technique is used because it is able to analyze several samples at a time. The polyacrylamide slab is prepared between two glass plates that are separated by spacers. Then a plastic “comb” is inserted into the top of the slab gel during polymerization, which forms indentations in the gel…

Critical Noncarcinogenic effect of acrylamide is classified as “Degenerative Nerve Changes”. This means that chronic ingestion of acrylamide resulted in an observable damaging of the central and peripheral nervous system, thus classifying it as a neurotoxin. This conclusion was determined after the results of two separate chronic (2year) drinking water studies performed on lab rats. (Friedman et al., 1995, 224307; Johnson et 14 al., 1986, 061340). The doseresponse data for acrylamide was…

of acrylamide in a wide variety of prepared foods (Tareke, E., et al. 2002). Since acrylamide was found to act as a carcinogen in laboratory animals and a neurotoxicant in humans, this finding created outrage around the world and brought focus to the matter of food processing safety (Council 2006). In the years following the first findings of high acrylamide levels, 3-MCPD and Furan were also among the main toxic agents identified as posing health concerns by health organizations around the…

Gel electrophoresis is used to separate DNA, RNA, and protein molecules by using an electric field. Protein gel electrophoresis is similar to agarose gel electrophoresis, but runs protein bands instead. The technique of protein gel electrophoresis uses cross-linked polymers of acrylamide (which is a neurotoxin and requires careful handling) or polyacrylamide to separate proteins and small nucleic acids. This technique takes longer than the agarose gels used to separate larger nucleic acids.…

GMOs are genetically modified organisms. There are lots of arguments about whether they are safe to eat or not. Most of the food we eat is GM. So are they safe to eat? Do the benefits outweigh the risks? Should they be labeled? I say yes to all three. I think that GMOs are safe to eat. "There currently is no evidence that there are material differences or safety concerns in available bioengineered foods." (FDA and AMA) though there have been some environmental uncertainty, like what makes the…

supernatant and keep the pellet for air drying  Dissolve the pellet in autoclaved distilled water SDS PAGE: SDS-PAGE, with full name of sodium dodecyl sulfate polyacrylamide gel electrophores, is the most widely used technique to separateproteins from complicated samples of mixture, plays key roles in molecular biology and wide range of subfield of biological research. Being present a electricity, proteins migerate towards the negative anode inside the poly-acrylamide gel under…

To make a 12.5% resolving gel, a mixture of 30% acrylamide, 0.8% bisacrylamide, 4X Tris HCl buffer with pH 8.8, DI water, 10% ammonium persulfate, and TEMED. The solution was poured into a gel caster, ethanol was added on top, and it was left alone to polymerize. A 4.5% SDS stacking gel was then prepared by mixing: 30% acrylamide, 0.8% bisacrylamide, 4X Tris HCl buffer with SDS pH 6.8, DI water, 0.25% bromophenol blue, 10% ammonium persulfate, and TEMED. The gel was left to polymerize and the…

crops (Duvauchelle). All this results in minimal pollution during the growing process and optimal crop production when harvested. The Innate Potato and the Golden Rice Project would be two prime examples justifying genetic modifications and their possibility to have a beneficial response in the health of the human population. Potatoes, as well as some other vegetables, release acrylamide, a natural occurring chemical, when cooked at high temperatures such as to make fries or potato chips.…

begin with the experiment, the gel caster was assembled. The two plates were properly cleaned and then introduced well align to avoid leakages to the plastic platform. Once there were correctly adjusted. A wash bottle was used to pour water to the edges and wait for about 5 minutes to verify there was no leakage. The TA came to the group in order to verify the correct assembly. Then, the water was through on the sink. 40 ml of 15% resolving gel solution was prepared. A 100 ml beaker was…

Acrylamide is a potentially carcinogenic (cancer – causing) substance that forms when coffee beans are roasted at high temperatures. The darker the roast the higher the levels of acrylamide are likely to be. In fact, coffee has been shown to be one of the major sources of this dangerous chemical in the diet. Obesity – there is a strong relationship between elevated cortisol levels and obesity. Cortisol may increase fat storage around the midsection. As I mentioned previously, coffee increases…