Acid-base homeostasis

Malachite Green Assay: The reaction that AGPase catalyzes produces PPi. The resulting PPi that is produced is hydrolyzed by an inorganic pyrophosphatase (Sigma Aldrich) to generate phosphate (Pi). Each reaction for the assay was conducted on a 96 well plate and the reaction mixture was 50µL and contained Pre-Mix which contained 50mM HEPPS, pH=8.0 (MP Biomedicals), 10mM MgCl2, 1.5mM FBP (Sigma Aldrich), and 0.2mg/mL Bovine Serum Albumin (Sigma Aldrich), and 1.5mU/µL yeast inorganic…

Introduction The objectives in this laboratory were to explore the titrations of several known acids and bases as well as observing the mole reactions of the chemical reactions that each compound undertook. The four following acids and bases were used to predict when a set amount of reactant would reach equilibrium: Hydrochloric Acid (HCl), H2SO4 (Sulfuric Acid), NaOH (Sodium Hydroxide), and Ba(OH)2 (Barium hydroxide). An unbalanced equation of each reaction was given so the balanced equation…

structure of a protein not only defines its shape and size, but also its function. Proteins consist of a long chain of 20 amino acids folded up into complex shapes. Each nonpolar amino acid folds into the interior of the proteins during the folding stage, as they are hydrophobic. The primary and secondary structure determines the hydrophobicity of a protein. Each nonpolar amino acid folds into the interior of the proteins during the folding stage. For instance, membrane proteins contain large…

either acid or base. Note: This activity may take more than one lessons. On the first lesson, students group, discuss and prepare their experiment. On the second lesson, students will have opportunities to carry out the investigation, then record, process, and evaluate their data. Preparing materials In the original experiment, hydrochloric acid and sodium hydroxide solutions have been used as acidic and basic solution, respectively. However, year 9 students might know that hydrochloric acid…

Determining the Chemical Formula of Hydrated Copper (Ⅱ) Sulfate Using the Empirical Formula Introduction In this experiment, the molecular formula of hydrated Copper (Ⅱ) Sulfate will be determined. In order to determine the molecular formula, the empirical formula is needed which is the ratio of moles of one substance to another. In this experiment, it is the ratio of Copper (Ⅱ) Sulfate to water. CuSO₄・XH₂O The empirical formula can be determined if the moles of the compared substances…

ACID-BASE PROPERTIES OF SODIUM CHLORIDE, SODIUM FORMATE AND AMMONIUM CHLORIDE INTRODUCTION The objective of this experiment is to examine the definitive characteristics of acids and bases by investigating the characteristics of strong versus weak acids; more specifically, why these differences are important in the process of titrations. In this experiment, there are two titrations performed and measured the pH of one salt solution. This experiment includes a strong acid and strong base…

concentrations 0.1M, 0.2M, 0.3M, 0.4M). // Hydrochloric acid (2M). // 100cm3 conical flask. // 10cm3 measuring cylinder. // Dropping pipette. // Small piece of plain paper with a large X drawn with think black pen. // Stopwatch. // Goggles. Variables: Independent - The concentration of sodium thiosulfate solution (0.1M, 0.2M, 0.3M, 0.4M). Dependent - The time taken for the cross to not be seen. Control - // Concentration and volume hydrochloric acid/ volume of sodium thiosulfate solution - to…

Experiments involving chemical reactions can be performed using simple household ingredients and products such as baking soda and vinegar. When baking soda (sodium hydrogen carbonate) reacts with ethanoic acid which is found in vinegar in a 1:1 ration, the reaction will produce three different products which are water, carbon dioxide and Sodium acetate. The reaction is: NaHCO3 + HC2H3O2 → NaC2H3O2 + H2O + CO2 In the experiment, the quantitative relationship between the two reagents which is the…

The objective of the lab was to determine the densities of known and unknown metal solids and liquids. For the liquids, initially, an Electronic Scale was placed onto Work Bench, and it was zeroed. An empty 50 mL Graduated Cylinder was placed onto the scale, weighing 54.00 grams. In increments of 10 mL. Until the volume was 30.00 Ml, 10.00 mL of water was poured into the Graduated Cylinder. In between each increase of 10.00 mL, the total volume and mass were recorded. For the known metals, the…

Analysis of Chemical Equilibria INTRODUCTION The reaction being done with borax and HCl is an acid base reaction. The K constant expression of the reaction is Kc = [Na^+][B4O7^-2]. A titration is needed for this experiment because when a strong acid with a known concentration is added to a weak base, the unknown concentration of the weak base can be found, which can then find the moles of the weak base, which can then find the Kc. The first law of thermodynamics is that energy can’t be created…