Acetic anhydride

3.7 ANALYTICAL METHOD FOR ESTIMATION OF RALOXIFENE HYDROCHLORIDE IN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY USING ACETONITRILE:0.05M AMMONIUM ACETATE (33:67) AS MOBILE PHASE 3.7.1 For Plain Drug Estimation Mode Reverse Phase HPLC Column C18 Detector UV Spectrophotometer Mobile Phase 0.05M Ammonium Acetate (pH 4): Acetonitrile Sample Raloxifene Hydrochloride in Methanol HPLC Flow Rate 1 mL/min Injection Volume 20µL Retention Time 4.6 min Λmax 289nm A stock solution of Raloxifene Hydrochloride was…

3. EXPERIMENTAL WORK 3.1 Materials andMethods 3.1.1 Chemicalsand standards used Table.No.15 List of chemicalsand standards used S.No. Chemicals Manufacturer Name Grade 1 Water Merck HPLC grade 2 Acetonitrile Merck HPLC grade 3 0. 22µ Nylon filter Advanced lab HPLC grade 4 0.45µ filter paper Millipore HPLC grade 5 Theo asthalin Cipla syrup form 6 Salbutamol sulphate and theophylline In - House In- House 3.1.2 Instruments used Table.No.16. List of instruments used S.No Instrument name Model…

A reaction mixture containing 0.5172g of boric oxide and 1.0167g of acetylacetone was prepared and mixed in a 10 mL beaker. The reaction mixture was heated with occasional stirring for 10 minutes. Next, another mixture containing 1.3321g tributyl borate, 3.0005g of vanillin and 15ml of ethyl acetate was prepared in a 50 mL conical flask. The mixture was mixed and a magnetic stirrer was added to the mixture. The mixture was then stirred on a hot plate for 5 minutes at room temperature. After…

Pantoprazole undergoes hepatic first pass metabolism , hence it shows poor bioavailability. The aim of study was to prepare and evaluate mucoadhesive tablet of pantoprazole using tamarind seed powder in combination with HPMC, magnesium oxide, and talc. Tamarind seed powder is used for dissolution enhancer (improve dissolution). Method: Six Formulations were developed with varying concentration of polymer like HPMC and other excipients like tamarind seed powder, magnesium oxide and lactose…

Isolation of Chlorophyll and Beta-Carotene from Plant Leaves Yokoyama Yuri B1 (A0142349E) 1. Aim: a) To separate beta-carotene and chlorophyll via column chromatography. b) To analyse the contents of the plant extract as well as the separated beta-carotene and chlorophyll via thin layer chromatography (TLC) c) To analyse the absorption peaks of both beta-carotene and chlorophyll using UV-Vis spectroscopy 2. Results: Fig 1. UV-Vis spectroscopy results for S2 (Beta-Carotene extract) Wavelength at…

concentration of 0.075%) was added and incubated for 1h. The supernatant was discarded and the cells in 96-well plates were washed with PBS twice to remove the neutral red that was not phagocytized by macrophages. Then cell lysate (ethanol and 0/01 % acetic acid at the ratio of 1∶1, 200 µl/well) was added to lyse cells. After cells were incubated at 4℃ overnight, the optical density at 490 nm was measured by a microplate reader. 2.6. Phagocytosis Assay by opsonized heat-killed baker's…

The main principle of manufacture of urea has two main reactions. 2NH3 + CO2 NH2COONH4 -37.4 Kcal/mol NH2COONH4 NH2CONH2 + H2O + 6.3 Kcal/mol While going main reactions, the undesirable side reaction taking place is 2NH2CONH2 NH2CONHCONH2 (Biuret) + NH3 This process is further complicated by the formation of a substance called biuret, NH2CONHCONH2, which should be kept low because it adversely affects the growth…

A process is disclosed for separating low molecular weight alcohols, especially ethanol, from aqueous mixtures  The feed mixture contains alcohol and water with an extractive solvent comprising a substituted and unsubstituted phenol having 6 carbon atoms and alcohol containing 2 to 4 carbon atoms.  In the process the upper stream contains water with minimum amount of solvent that is 99.9% and the bottom stream contains alcohol with minimum amount of water.  The temperature of the process is…

the Diels-Alder reaction will be performed by reacting (E,E)-2,4-hexadien-1-ol with maleic anhydride without the use of a solvent to make the reaction more greener. The reaction will be tested for the presence of a carboxylic acid in the product, to make sure the reaction proceeded as expected, with the use of sodium bicarbonate (NaHC"O" _"3" ). Reactions Reaction 1: Diels-Alder reaction of maleic anhydride and (2E, 4E)-2,4-hexadien-1-ol…

Measuring the mass of Iron in an iron sulphate tablet using REDOX titration Aim The aim of this experiment is to determine the mass of iron present in one iron sulphate tablet using REDOX titration under acidic conditions. Safety H2SO4: This is an irritant which may cause burns on skin and eyes; therefore care must be taken when handling this chemical. Protective gear (gloves, lab coat and safety goggles) must be worn at all times. If it comes in contact with skin or eyes, it should be…