Absorbance

incubated, washed and blocked with BSA. Again plate was washed, sample and IL-6 was added, incubated, washed and detection antibody was added, incubated and washed. Avidin- HRP was added, incubated and washed. SuperAqua Blue solution was added and absorbance was measured. Use of substances:In ELISA, bovine serum albumin (BSA) was used as a blocking agent to avoid non-specific binding of antigens and antibodies to the microtiter wells. Washing buffer was used to remove, the coating material. In…

Efaverinz+Polysorbate-80 IR spectra of Efaverinz+PEG-400 IR spectra of Efaverinz+MCC+AEROSIL Fig no: 10 IR spectra of the Optimized Formulation Table 10: STANDARD CALIBRATION CURVE FOR EFAVERINZ IN 6.8 PHOSPHATE BUFFER of 2% SLS: CONCENTRATION (µg/ml) ABSORBANCE (nm) 0 0 2 0.077 4 0.138 6 0.211 8 0.281 10 0.352 Figure 11:Standard calibration curve for…

Figure 3.1.2. Antibody titres of rabbit antisera. The antisera were collected from immunized rabbits and antibody titres measured by indirect ELISA as described under Methods (section 2.2.3.1). 3.1.2.1.3 Purification of α-LA, HRP and human erythrocyte specific polyclonal IgG The IgG fractions were purified from rabbit sera by ammonium sulphate precipitation followed by negative ion exchange chromatography employing DEAE-cellulose. The antisera were decomplemented, IgG fraction precipitated by…

Serratia marcescens is one of many species that have been shown to cause infection even though it was once considered as non-pathogenic. Therefore, it is crucial that we find an effective method of controlling these bacteria. Previous studies have already shown that aminoglycosides such as Streptomycin are effective against S. marcescens, but it is also necessary to administer the antibiotic at certain concentrations for minimizing bacterial growth. In this experiment, we attempted to find a…

The electrochemical properties of the squaraine dyes (SQ1 & SQ2) were investigated. The effects of the chemical structures of two SQ1&SQ2 dyes were selected in relation to the variable property of -COOH substituent for SQ1 compare to SQ2 which without substituent and determine their potential energy levels in ground and excited states HOMO and LUMO. The HOMO energy levels of the SQ1 & SQ2 dyes are -5.19 eV and -5.16 eV. The LUMO energy levels are calculated to be -3.08 eV and -3.1eV respectively…

% further, PHA yield from total biomass was increased by 39 %. Higher productivity of PHA from glycerol was due to the reduced nature than glucose.Both biomass and PHA productivity level was higher than glucose.FTIR Showed the maximum relative absorbance at 1742 to confirm the polymer…

Introduction The percentage of men over 40 using testosterone replacement therapy (TRT) tripled from 2001 to 2011¹. Newer, gel based forms of treatment have aided the ease of application and encouraged prescription rates. Along with this trend comes an increase in the data surrounding TRT. This influx adds to the large scale, randomized studies conducted on the use of TRT in hypogonadal men. TRT is widely accepted to provide relief from retarded spermatogenesis, low sex function, depression,…

Early studies on the lateral diffusion of rhodopsin in ROS membranes resulted in viewing this visual receptor as monomeric entity freely diffusing in a phospholipid bilayer (Calvert et al., 2001; Chabre et al., 2003; Liebman et al., 1982; Poo and Cone, 1974). However, it is important to stress that the movement of rhodopsin restricted to approximately two-dimensional environment of membranous phospholipid bilayer would rather reduce its dynamic freedom by orders of magnitude relative to soluble…

electrophiles, scavenging free radicals and providing a reservoir for cysteine. The sulfhydryl group of GSH reduces 5-5'-dithiobis (2-nitrobenzoic acid) (DTNB) and form the yellow colour product 5-thio-2-nitrobenzoic acid (TNB)21. Measurement of the absorbance of TNB at 412 nm provides an accurate estimation of GSH in the sample. Figure 7. Materials 5×105 cells/mL cell suspension Culture medium, BSA, PBS (Ca2+, Mg2+ free), FBS, trypsin-EDTA, L-glutamine, antibiotic-antimycotic solution (10,000…

Cerium is a rare earth element of the lanthanide series (atomic no. = 57– 71) of the periodic table. Among the various rare earth metals, cerium granted a hugeattention towards scientific community due to its catalytic antioxidant property. Usually, antioxidants protect cells from oxidative damage, mediated by ‘reactive oxygen species’ (ROS), which include free radicals such as superoxide anion (O2•-), hydroxyl radical (•OH), singlet oxygen (1O2) and non-free radical species like hydrogen…