Analytical Essay

HPLC column can be categorized into two types • Reverse Phase • Normal Phase 3.4.1.1. Reverse Phase Chromatography Reverse Phase system has a non-polar stationary phase over which polar solvents are used as mobile phase; it is generally used for separation of polar species. 3.4.1.2. Normal phase chromatography Normal Phase system has a polar stationary phase over which non-polar solvents are used as mobile phase; it is generally used for separation of non-polar species.[38]. A detection…

The film, “Jaws”, was released in 1976, by Stephen Spielberg and is widely considered the first Hollywood Blockbuster. “Jaws” is one of my if not my favorite movie, I have seen this film, as many as a half dozen times and each time I view it, I find that I enjoy it more and more. “Jaws” has a unique way of captivating any audience who views the film, its director Stephen Spielberg, is a master at grabbing the audience’s attention by making us feel as if we are the ones in the water at the Amity…

ABSTRACT Flakiness & Elongation index is one of the most prominent criteria that govern behavior and performance of aggregate in the bituminous mixes. The strength and serviceability requirements of bituminous mixture such as Stability, Flow, Voids in Total mix (VTM), Voids Filled with Bitumen and (VFB) highly depend on the physical properties of aggregates. This study conducted by observing the effect of flakiness & elongated index by adding different percentages from 0% to 50% of flaky &…

Introduction Thin-layer chromatography, otherwise referred to as TLC, is a fast and inexpensive way to determine the amount of components in a solution or to identify an unknown compound. The principal behind TLC is when different compounds are placed on silica gel or alumina, (stationary phases) they will travel different distances when eluted with the proper solvent. According to ChemistryGuide, this is because the more polar compound has a stronger interaction with the silica gel due to its…

The human body produces various essential proteins that provide functionality in the biochemistry of our system. How individual molecules are separated or a mixture of molecules are separated can be dependant of their size. Size exclusion chromatography (SEC), is the controlled separation technique that is based on the size of the particles in relation to the pore size (Barth and Saunders 2012). The separation process veers to be quick for large molecules and slower for smaller molecules. The…

Chromatography is a process used to separate molecules on the basis of chemical or physical properties, such as molecular mass, charge, or hydrophobicity. (Ninfa 121) There are many types of chromatography processes that can be used in different experiments and scenarios. These chromatography processes on include Gel filtration, ion exchange, and high pressure liquid chromatography. However, in this laboratory session the process of affinity chromatography is utilized to purify a His-tagged…

Ali Youssef CHM 111-0A7L Mitra Jahangeri Paper Chromatography Lab Discussion The results of the Paper Chromatography process seem to have aligned with what was expected. When the capillary action on the paper had completed and the chromatograph was finished, the distance that each cation moved (D), as well as the distance that the solvent moved (L), could be properly measured. The distance that the solvent moved ended up being 7.05cm, a number that would represent the constant variable L in…

Egypt The Egyptian outlook on perfumes could be derived from their lifestyle learned from the records, the objects found in connection to death and afterlife and mummified remains. The ancient Egyptians were concerned with the odours of everyday life and death. Dated to 1550 BCE, the Ebers papyrus gives us a wealth of knowledge on Egyptian medical preparations for ailments, perfumed anointing oils, and incense plants. As we move back through time, as early as the third millennium BCE, it…

4.2.3.5. Antioxidant assays in gastric tissue homogenate 4.2.3.5.1. Catalase activity Catalase activity in the gastric tissue was measured according to the procedures of Sinha (1972). One ml of homogenate solution was taken and mixed with 5 ml of phosphate buffer of pH 7.4. To this 4 ml of 0.2 M H2O2 was added and time noted. Exactly after 180 seconds of adding H2O2, a set of 1ml of reaction mixture from the above was taken and 2 ml dichromate acetic acid was added. It was then kept in a boiling…

Gas chromatography (like any other chromatography) is a separation technique in which the mobile phase is a gas. This technique is applied for the separation of volatile substances. This method separates a mixture into its components and is used to measure the concentration as well as it identifies the components. GC creates a time separation rather than physical separation which means that the components are separated in time. After they are detected, a chromatogram is generated. Each peak in…