Analytical Essay

like molecular weight, density, shape, etc. If it is used for the separation of one type of materials from others is termed as preparative centrifugation and when used for measurement of physical properties of macromolecules, then it is known as analytical centrifugation. Principle of centrifugation: Particles suspended in a solution are pulled downwards by Earth’s gravitational force. In a solution, particles with higher mass…

MDMA (3,4-methylenedioxymethamphetamine), commonly referred to as ecstasy, and methamphetamine are central nervous system stimulants, which can produce euphoria, enhanced mental and emotional clarity, sensations of lightness and floating, and other hallucinations. (Chandra et al. 2015) Due to their effects, MDMA and methamphetamine are used in Drug-Facilitated Crimes (DFC), intended to impair behaviour, perceptions, or decision-making capacity to take advantage of an impaired person without…

Paper Chromatography Paper Chromatography is an analytical method that is used to separate coloured chemicals substances, especially pigments. This is how paper chromatography is conducted: Materials Chromatography Paper (Can also be Coffee Filter) Large Beaker (max one litre) Paper Clips/Sellotape & Skewer Stick Water soluble black marker Water Procedure: Draw a ink dot with the marker on each one of the chromatography paper near the bottom end of the paper. Then stick or paper clip the…

have the potential to bind to other proteins. Once this red colored species bind to a protein the bound dye molecule transforms to blue species, and this color change is the one measure by the spectrophotometrical. The assay is a spectroscopy analytical technique based upon the absorbance maximum for an acidic solution of Coomassie Brilliant Blue. From the assay is observed the maximum absorbance for an acidic solution of Coomassie Brilliant Blue G-250 mostly shifts from 465 to 595 nm upon…

compound with high reproducibility. The materials for constructing a HPLC column are inert to the analytes such as polished stainless steel, glass and PEEK polymers. There are mainly four types of columns used in HPLC which are high performance analytical column, preparation column, capillary column and…

Carl Jung was a Swiss psychiatrist who established the body of analytical psychology. He stated that every person has several personalities within oneself. Basically, we can look at one’s personality from four different categories, persona, shadow, anima/animus and archetypes. The easiest to recognize is persona. Persona is a social mask that we all show to the public. We change the roles in different situations in society. For instance, a student acts as an attentive respectful person who…

Analytical Method Validation of Sylimarin The λmax of Sylimarin in ethyl alcohol and PBS was found to be 286 nm and 322nm, respectively. The drug follows linearity in the concentration range 2–20 μg/ml with a correlation coefficient value of 0.998 and 0.999 for in ethyl alcohol and PBS, respectively. The accuracy of the method was checked by recovery. The % recovery was found to be in the range of 99.54– 100.98% in ethyl alcohol and 98.94– 101.4% in PBS, respectively. The precision of the…

Introduction: LC-MS is used for the bioanalysis of small molecules drugs, which has molecular mass of less than 800 Da. Now a days, it is also frequently used for the bioanalytical determination of biopharmaceuticals.1 In pharmaceutical R & D, it takes a year to develop a new marketing drug identified from thousands of new compounds. The drug discovery is divided into early and late phase, during which pharmacokinetics study or bioanalysis of a drug is done. Once, the bioanalysis of a drug is…

Introduction Chromatographic process is a separation technique which has to do with the mass transfer of test sample between a stationary and mobile phase. HPLC (High Performace Liqud Chromatography) is a vast system which involves the use of a mobile and stationary phase to separate materials. The stationary phase is usually a column packed with solids (usually silica gel) while the mobile phase is usually a solvent or a mixture of various solvents. The mobile phase usually, is the carrier of…

In Lisel Mueller’s poem “Reading the Brothers Grimm to Jenny,” the narrator details the relationship that he or she has with Jenny. Jenny is a character who is facing her own internal conflicts, but displays innocence and truthfulness to the narrator. As a result of this display, the narrator is conflicted about telling fairytales to Jenny. Moreover, the narrator is concerned with the high qualities and ideas that she attributes to people who are not worthy, who the narrator deems unworthy. To…