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hyperoxia (95% O2). OptiMEM media was used to treat the cells. The supernatant, consisting of the media that the cells were grown in, was collected and placed into labeled 15 mL falcon tubes. Next, the tubes were centrifuged for 5 minutes in 4°C at 1000 rpm. 1800 µL of the supernatant was collected from falcon tubes and transferred to labeled centricons. Centricons were centrifuged for 40 minutes in 4°C at 4200 rpm. Centricons were removed and the liquid in the top cone was collected and…

SCENARIO TWO Frank was a customer at Friendly Seafood Restaurant owned by Friendly Eats, Inc. While consuming a bowl of chowder at the restaurant, Frank chipped a tooth on a shell accidentally left in his soup by the chef. He then had to spend $1000 to have his dentist put a crown over that chipped tooth. 1. Name the cause of action in tort that Frank could bring against Friendly Eats, Inc. NEGLIGANCE 2. List each element of that tort. Next to each element, state the facts from the above…

The actions of the Shay’s Rebellion went too far when they disagree with the government taking their land and crops. When the government took their land the framers had no more rights. They had trouble selling their goods and they had trouble paying the money requested by the states. As a result, the state attacked the framers’ land to pay their debts. Some of the farmers wanted to government to have a problem with paper money. On page 195 in the textbook it states, “Surely your honours are not…

Shay’s Rebellion made a big change in history. The question, do you believe in the actions of members Shay’s Rebellion or do you think their actions went too far? Well, Shay’s Rebellion would have been simple if the government did what the people wanted. But, they didn't, so that led to some trouble. In the school textbook on page 195 it states that “American farmers suffered because they could not sell their goods. They had trouble paying the money requested by the states meet Revolutionary War…

Q3) For demographic segmentation, it is appropriate to apply the income variable to compare the likely target market segments between the two watches. A smartwatch will likely to attract customers earning an annual income of USD$20,000 - USD$1,000,000. Meanwhile, a luxury watch is expected to entice customers earning an annual income of USD$1,000,000 and more. According to TechTimes.com, the most expensive smartwatch in the market belongs to LG’s G Watch R which costs around USD$400…

In more specific terms the prevalence among children was 7.4 per 1000 and adults was 17.7 per 1000 patients [16]. SCD patients in general have a 3.75% chance of having a stroke throughout their life [6]. Hemorrhage strokes, which are more common in adults, are most likely to occur between the ages of 20 to 29. Patients who experience…

Cell phones are undoubtedly one of the best inventions in the history of mankind. They basically give us access to the whole world right there at our finger tips. We can search for whatever we want in a matter of seconds, talk to anyone on almost every inch of the globe, and we can do countless other things. In a nutshell, cell phones have definitely made many areas of our lives easier. However, cell phones are damaging one aspect of our day to day lives. They may give us extraordinary access…

in Kindergarten age children ("Vaccination Coverage Among Children in Kindergarten — United States, 2012–13 School Year," 2013). Vaccinations have been credited with saving thousands, if not millions, of lives since their inception by the Chinese in 1000 CE. In 1796, Edward Jenner successfully created an immunity to smallpox, and by the 1930’s there were vaccines for measles, mumps, rubella and polio. No longer did America’s children…

1.0. INTRODUCTION Neonatal admission generally refers to the admission of newborns under 29 days old into a health facility for medical care. Because neonates are fragile and yet to develop competent immune system, they are prone to infections and most of the illnesses they acquire usually require critical care, hence their admission to the neonatal and intensive care units (NICU) (Gupta et al., 1993). Neonatal infections may begin in-utero, early in labour or postpartum (U.N IGME, 2014).…

local franchise, has lower monitoring cost because it is just small store and therefore doesn’t need to pay higher wages. Moreover, efficiency wages can save employers from the turnover cost. Turnover cost can be very high for employers. Assume that 1000 workers in Apple would want to leave the firm because of…