substrates (Table 1). Table 1: Substrate specificity of PPO enzyme Substrates Specific activity (units mg-1 protein) Km (µM) Vmax (abs min-1) Catechol 2300 4.12 X 10-3 3020 L-DOPA 430 16.40 X 10-3 990 L-Tyrosine 1930 6.12 X 10-3 2930 P-Cresol 2110 5.01 X 10-3 2983 Effect of Inhibitors The effects of various compounds on the PPO activity were provided in Tables 2. Enzyme activity was markedly…
You already know your pool has a pump, but do you really know what that pump actually does? You might be surprised! What Exactly Does a Pool Pump Do? Your pool pump is the heart of your pool and without it, it would die...so to speak. Just like a human heart, your pool's heart (the pump) only has one thing it has to do which is pump water. However, that one thing serves several very important functions, such as: • Pulls the water from your pool through the skimmers and drains. • Then it…
performed using the Statistical Package for Social Science (SPSS) version 10. Statistical results of the optimum percent swelling are depicted in Table 1. The optimized parameters are explained as follow: 1. Effect of initiator concentration: The effect of initiator concentration on the grafting efficiency of synthesized hydrogel is shown in Figures 1. It is…
automatically adjusting hydrogen peroxıde Colloıdal-Ag dose to reduce colonization and growth of heterotrophic bacteria in dental unit waterlines and wastewaters. Thirty dental units were assigned into three groups with respect to the protocol employed: Group 1: 10 units were selected for automatic administration. Group 2: 10 units were selected for manual administration and Group 3: 10 units served as controls. Water samples from DUWLs and waste waters were collected every week during the ten…
Water quality dynamics in an urbanizing subtropical estuary (Oso Bay, Texas) Estuarine environments like that of Oso Bay in Corpus Christi, Texas are subject to fluctuations in geochemistry and relative water quality based on factors related to extreme weather events, changing water flow and human impacts. A 2011-2015 study on the water quality dynamics of Oso Bay found fluctuations in the estuarine geochemistry were related to some of these factors, particularly human influences. A wastewater…
Gasmalla et al Trop J Pharm Res, January 2014;13 (1): 61 Tropical Journal of Pharmaceutical Research January 2014; 13 (1): 61-65 ISSN: 1596-5996 (print); 1596-9827 (electronic) © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved. Available online at http://www.tjpr.org http://dx.doi.org/10.4314/tjpr.v13i1.9 Original Research Article Nutritional Composition of Stevia rebaudiana Bertoni Leaf: Effect of Drying Method Mohammed Abdalbasit…
Introduction The purpose of this lab was to identify the lipids in an egg yolk and to identify the amount of lipids in milk [1]. To extract the lipids from the egg yolk, the egg yolk needed to be combined with an organic solvent such as a mixture of CHCl3/MeOH. An organic solvent was needed to carry the extracted lipids up the TLC plate because the plate was polar, due to the presence of silica gel (silanol groups). To compare relatively similar lipids to the ones found in the egg yolk, the egg…
the temperature at a range between 25-55 °C (Pasupuleti & Braun, 2010). During alkaline hydrolysis of proteins, some amino acids like serine and threonine are damaged during alkaline hydrolysis, however, tryptophan remains intact. Also, this process causes racemization of amino acids, by modifying its structure and converting L-amino acid in its isomeric forms D-amino acids (Wisuthiphaet & Kongruang, 2015). Disulphide bonds are also fragmented with loss of cysteine, serine, and threonine and…
One of the parameters that determines water quality is pH. It is defined as the negative logarithm of the hydrogen ion and is intended to be a measurement of the balance of acids and bases. Temperature affects pH. In pure water, a decrease in pH of 0.45 occurs when temperature is increased by 25°C [11]. There are several methods for measuring pH. The most common is the use of a glass electrode sensible to it, a reference electrode and a measurement device. Another method is the use of…
The purpose of this experiment was to learn techniques to determine the freezing point of a pure benzophenone, a solvent. Furthermore, methods for determining the freezing point depression in relation to the solute, cyclohexanone, concentration were employed in addition to learning what molality is and how it related to the colligative properties being studied. These objectives were achieved by melting both pure benzophenone and benzophenone/cyclohexanone solutions (two solutions with…