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7 Cards in this Set
- Front
- Back
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Test for proteins |
Add biuret reagent From blue to lilac if protein |
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Reducing sugar test |
Add benenicts |
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Non reducing sugar test |
Add dilute hcl and boil in water bath 2 min
Neutralise with sodium hydrogen carbonate
Do benedicts test
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Starch test |
Add iodine solution Blue black means starch Orange means no starch |
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Lipid test emulsion test |
Add ethanol . Shake |
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Describe how concentration of glucose may be determined . The solution that goes into colorimeter |
More reducing sugar present, more presipitate formed; more benedicts ( copper sulfate) used up; if precipitate filtered out; conc of remaining solution says how much benedicts used up The lower the conc of filtered solution , the higher the conc reducing sugar present |
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Describe how calorimeter works |
Shines beam of light Photoelectric cells gives reading of how much light has passed through Water calibrates calirometer 100% transmission and 0 absorbtion Plot transmission against reducing sugar conc to get calibration curve |
