Week 4 Medium Info

Front 1

Starch Hydrolysis:

Symbol

Back 1

Amylase.

Front 2

Starch Hydrolysis:

Substrate

Back 2

Starch.

Front 3

Starch Hydrolysis:

Reaction

Back 3

Hydrolysis.

Front 4

Starch Hydrolysis:

Indicator

Back 4

Iodine.

Front 5

Starch Hydrolysis:

(+) Results

Back 5

Clearing in agar following iodine.

Front 6

Starch Hydrolysis:

(-) Results

Back 6

No clearing.

Front 7

Starch Hydrolysis:

Used to Isolate...

Back 7

ID Neisseria.

Front 8

DNA Hydrolysis Test:

Symbol

Back 8

Dnase.

Front 9

DNA Hydrolysis Test:

Substrate

Back 9

DNA.

Front 10

DNA Hydrolysis Test:

Reaction

Back 10

Hydrolysis.

Front 11

DNA Hydrolysis Test:

Indicator

Back 11

Methyl Green.

Front 12

DNA Hydrolysis Test:

(+) Results

Back 12

Clearing of green in agar.

Front 13

DNA Hydrolysis Test:

(-) Results

Back 13

No clearing.

Front 14

DNA Hydrolysis Test:

Used to Isolate...

Back 14

Diff. Serratia from Enterobacter.

Front 15

Lipid Hydrolysis Test:

Symbol

Back 15

Lipase.

Front 16

Lipid Hydrolysis Test:

Substrate

Back 16

Lipids (fats).

Front 17

Lipid Hydrolysis Test:

Reaction

Back 17

Hydrolysis.

Front 18

Lipid Hydrolysis Test:

Indicator

Back 18

None.

Front 19

Lipid Hydrolysis Test:

(+) Results

Back 19

Clearing of media around growth.

Front 20

Lipid Hydrolysis Test:

(-) Results

Back 20

No clearing.

Front 21

Lipid Hydrolysis Test:

Used to Isolate...

Back 21

Detect lipolytic bacteria in milk products.

Front 22

Caesin Hydrolysis Test:

Symbol

Back 22

Casease.

Front 23

Caesin Hydrolysis Test:

Substrate

Back 23

Caesin.

Front 24

Caesin Hydrolysis Test:

Reaction

Back 24

Hydrolysis.

Front 25

Caesin Hydrolysis Test:

Indicator

Back 25

None.

Front 26

Caesin Hydrolysis Test:

(+) Results

Back 26

Clearing of media around growth.

Front 27

Caesin Hydrolysis Test:

(-) Results

Back 27

No clearing.

Front 28

Caesin Hydrolysis Test:

Used to Isolate...

Back 28

Grow casease positive bateria.

Front 29

Gelatin Hydrolysis Test:

Symbol

Back 29

Gelitinase.

Front 30

Gelatin Hydrolysis Test:

Substrate

Back 30

Gelatin.

Front 31

Gelatin Hydrolysis Test:

Reaction

Back 31

Hydrolysis.

Front 32

Gelatin Hydrolysis Test:

Indicator

Back 32

None.

Front 33

Gelatin Hydrolysis Test:

(+) Results

Back 33

Liquid media.

Front 34

Gelatin Hydrolysis Test:

(-) Results

Back 34

Gelatinous media.

Front 35

Gelatin Hydrolysis Test:

Used to Isolate...

Back 35

ID gelitinase positive bacteria.

Front 36

Urea Hydrolysis:

Symbol

Back 36

Urease.

Front 37

Urea Hydrolysis:

Substrate

Back 37

Urea.

Front 38

Urea Hydrolysis:

Reaction

Back 38

Hydrolysis.

Front 39

Urea Hydrolysis:

Indicator

Back 39

Phenol Red.

Front 40

Urea Hydrolysis:

(+) Results

Back 40

Pink - Strong Urase positive; Orange - Weak Urease Pos.

Front 41

Urea Hydrolysis:

(-) Hydrolysis

Back 41

Yellow.

Front 42

Urea Hydrolysis:

Used to Isolate...

Back 42

ID Proteus from other enterics.

Front 43

SIM Medium:

Symbol

Back 43

SIM.

Front 44

SIM Medium:

Substrate

Back 44

Sulfer, Cystein.

Front 45

SIM Medium:

Reaction

Back 45

Reduct, Hydrolysis.

Front 46

SIM Medium:

Indicator

Back 46

Iron, Kovak's Reagent.

Front 47

SIM Medium:

(+) Results

Back 47

Black precip. - Sulfer pos.; Red Reagent - Indole Pos.; Motility - Hazy media.

Front 48

SIM Medium:

(-) Results

Back 48

Idk.

Front 49

SIM Medium:

Used to Isolate...

Back 49

Differentiate between Enterobacteriacaea.

Front 50

Kligler Iron Agar:

Symbol

Back 50

TSIA.

Front 51

Kligler Iron Agar:

Substrate

Back 51

Sugar, Sulfer, proteins.

Front 52

Kligler Iron Agar:

Reaction

Back 52

Ferm., Reduc., Oxid.

Front 53

Kligler Iron Agar:

Indicator

Back 53

Phenol Red, Iron.

Front 54

Kligler Iron Agar:

(+) Results

Back 54

5 possible combinations.

Front 55

Kligler Iron Agar:

(-) Results

Back 55

Idk.

Front 56

Kligler Iron Agar:

Used to Isolate...

Back 56

Differentiate between Enterobacteriacaea.

Front 57

Kligler Iron Agar:

Example Results

Back 57

Left to right:

alkaline slant/acid butt (KIA);

alkaline slant/no change in the butt (K/NC);

uninoculated control;

alkaline slant/acid butt, hydrogen sulfide present (KIA, H2S);

and acid slant/ acid butt, gas (NA, G).

Front 58

Kligler Iron Agar:

Yellow slant/yellow butt-KIA (A/A)

Back 58

Glucose and lactose fermentation with acid accumulation in slant and butt.

Front 59

Kligler Iron Agar:

Yellow slant/yellow butt-TSIA (A/A)

Back 59

Glucose and lactose and/or sucrose fermentation with acid accumulation in slant and butt.

Front 60

Kligler Iron Agar:

Red slant/yellow butt (K/A)

Back 60

Glucose fermentation with acid production; proteins catabolized aerobically (in the slant) with alkaline products (reversion).

Front 61

Kligler Iron Agar:

Red slant/red butt (K/K)

Back 61

No fermentation; peptone catabolized aerobically and anaerobically with alkaline products; not from Enterobacteriaceae.

Front 62

Kligler Iron Agar:

Red slant/ no change in the butt (K/NC)

Back 62

No fermentation; peptone catabolized aerobically with alkaline products; not from Enterobacteriaceae.

Front 63

Kligler Iron Agar:

No change in slant/ no change in butt (NC/NC)

Back 63

Organism is growing slowly or not at all; not from Enterobacteriaceae.

Front 64

Kligler Iron Agar:

Black precipitate in the agar (H2S)

Back 64

Sulfur reduction (an acid condition, from fermentation of glucose or lactose, exists in the butt even if the yellow color is obscured by the black precipitate).

Front 65

Kligler Iron Agar:

Cracks in or lifting of agar (G)

Back 65

Gas production.