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13 Cards in this Set
- Front
- Back
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Describe LINEs |
1. autonomous retrotransposons 2. comprise 21% of the human genome 3. 6.1kb (L1) element containing 2 open reading frames (ORF1 & ORF2) |
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describe ORF1 and what it encodes for |
ORF1 is 1kb long and encodes an RNA binding protein |
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describe ORF2 and what it contains |
ORF2 is 4kb long and contains a reverse transcriptase and endonuclease domain |
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what is the C-value and what is it measure in |
amount of DNA contained within a haploid nucleus. it is measured in kilobases and picograms |
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what is the C-value paradox |
the lack of relationship between the DNA content (c-value) of an organism and its coding potential |
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what is the melting temperature (Tm) ? |
the temp at which DNA is half unfolded |
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what is the rate (speed) of reannealing governed by? |
1. DNA concentration 2. DNA fragment length 3. DNA sequence composition and complexity |
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what is DNA reannealing measured by? |
Cot value (molsec-1) |
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what is the longest tandem array known in humans? and how long is it? |
human alphoid length - 5 x 10^6 bp |
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what are the 3 types of tandemly repeated DNA |
satellite minisatellite microsatellite |
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what is unequal crossing over? |
-nonreciprocal recombination -results from an error in pairing and crossing over in which non-equivalent sites are involved in a recombination event -it produces one recombinant with a deletion of material and one with a duplication |
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what is replication slippage? |
-it occurs when the daughter strand slips back one repeating unit in pairing with the template strand -the extra repeat is extruded as a single strand loop -replication of this daughter strand in the next cycle generates a duplex DNA with an increased number of repeats |
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name some protein coding genes |
1. immunoglobins 2. collagen 3. keratins 4. histones 5. ribosomal proteins 6. actins, myosins and tropomyosins |
