Virology Quiz 2 Old Questions

Front 1

What is detected by the FELV snap Elisa used diagnostically to detect cats infected with FeLV
a) p27 protein
b) ab to p27 protein
c) gp 70 glycoprotein
d) ab to gp 70 glycoprotein

Back 1

a) p27 protein

Front 2

enterocolitis is a syndrome that is sometimes observed in cats that are felv infected and viremic. it is characterized by bloody diarrhea and anemia and has been associated with
a) FIV
b) feline panleukopenia virus
c) feline coronavirus
d) rotavirus

Back 2

b) feline panleukopenia virus

Front 3

what three epidemiological factors are considered most important in determining if a cat that is infected with felv will be unable to fight off the infection and become viremic

Back 3

age-younger
duration of exposure (high)
exposed to high concentration of virus

Front 4

an felv positive viremic cat that has been diagnosed with a lymphoma will most likely test Elisa (pos/neg), IFA (pos/neg), FOCMA ab (pos/neg), VN ab (pos/neg)

Back 4

Elisa positive
IFA positive
Focma ab negative
VN ab negative

Front 5

a cat is presented with anorexia and weight loss lasting about 2 weeks. you test the cat for felv by the snap test and it is positive. of the following clinical signs or blood results, which would you least expect to observe in this FELV positive cat?
a) lymphadenopathy
b) stomatitis and gingivitis
c) hypergammaglobulinemia
d) normocytic, normochromic anema
e) presence of numerous lymphoblasts in the blood smear

Back 5

c) hypergammaglobulinemia

Front 6

which of the ofllowing is true regarding felv infection in cats?
a) alimentary lymphosarcoma is more frequently seen in young cats that test elisa positive for felv
b) after exposure to felv, older cats are more likley to become viremic and shed felv than younger cats
c) more felv infected cats die of non neoplastic felv complications than of felv neoplasia
d) chronic regenerative anemia is the most common presenting sign in cats that test elisa positive for felv
e) felv is transmitted between cats primarily through bites during fights

Back 6

c) more felv infected cats die of nonneoplastic felv complications than of felv neoplasia

Front 7

a 6 month old cat is diagnosed with a thymus lymphosarcoma at necropsy. the peripheral blood cells and tumor cells are positive for felv by pcr. with this history the cat should test negative for:
(there may be more than one answer)
a) circulating focma ab
b) circulating virus neutralizing (vn) ab
c) felv antigen as determined by elisa using the snap test
d) infected circulating lymphocytes as determined by the ifa test on a blood smear
e) none of the above- the cat should test positive for all of the above

Back 7

a) circulating focma ab
b) circulating vn ab

Front 8

which clinical sign is least likely to be associated with felv infection in cats
a) thymus atropy
b) immunosuppression
c) acute lymphocytic leukemia
d) erythrocytosis (high rbc count)
e) lameness due to solitary bone tumor

Back 8

d) erythrocytosis

Front 9

the three most important factors that will determine if a cat, exposed to felv, beomes infected and viremic are

Back 9

age- younger cats more susceptible
exposure time- must be prolonged
dose- must be high

Front 10

the saliva of an emaciated, anemic 8 year old tomcat is tested and found to contain lots of infectious felv particles. blood from the cat is submitted to the diagnosted lab to perform an ELISA test to detect antigenemia, a neutralizing antibody test to detect the present of viral neutralizing ab, nad an indirect fluroscent antibody test to detect viral infected lymphocytes. what set of results would you expect on this cat?
a( elisa pos, IFA pos, NAT pos
b) elisa pos, ifa pos, nat neg
c) elisa neg, ifa neg, nat pos
d) elisa neg, ifa pos, nat neg
e) elisa pos, ifa neg, nat pos

Back 10

b) elisa pos, IFA pos, NAT neg

Front 11

which of the following is incorrect regarding rabies inclusion bodies. these IBs
a) are obsreed in the brain cells of all animals that die from rabies infections
b) are located in the cytoplasm of neurons
c) are called negri bodies
d) conist of accumulation of viral nucleocapssid proteins
e) can be seen with both light and EM microscopes

Back 11

a) are observed in the brain cells of all animals that die of rabies virus infection

Front 12

there is one felv asociated malignancy that is primarily seen in older cats that often test felv negative by the snap elisa test. which is it?
a) thymic lymphosarcoma
b) alimentary lymphosarcoma
c) acute lymphocytic leukemia
d) multicentric lymphosarcoma
e) lymphosarcoma involving the cns

Back 12

b) alimentary lymphosarcoma

Front 13

felv infected cells can be recognized and killed by cells of the immune system by a process called ADCC. the first event that must occur in order for ADCC to proceed is the binding of the felv infected cells by _____ (a molecule). the viral component to which this molecule binds is the ________. the next stage is recognition of the binding molecule by cells. one important cell responsible for ADCC is _______

Back 13

antibodies
glycoprotein (GP70)
macrophages

Front 14

an felv positive viremic cat that has been diagnosed with a lymphoma will most likley test Elisa (pos/neg), IFA (pos/neg), focma ab (post/neg), VN ab (pos/neg)

Back 14

elisa positive
IFA positive
FOCMA ab negative
VN ab negative

Front 15

felv is excreted primarily in the ____ of viremic cats. an infected cat will clear the felv infection if it develops antibodies to the _____ (A viral protein) with ____ weeks after infection, before the virus ahs a chance to infect the cells in the ____ _____. during this time the cat will test (pos/neg) by the snap elisa test. however, if the cat does not develop Ab then it can become persistenly infected. persistenly infected cats have a ____ chance of dying of felv associated diseases with ___ years of infection. these persistently infected cats often develop a _____ anemia

Back 15

saliva
gp70
3 weeks
bone marrow
positive
t80%
3 years
non regenerative

Front 16

the recombinant vaccinia irus that is used to vaccinate raccoons against rabies is immunogenic. its immunogenicity is due to the fact that the recombinant virus that the raccoons are vaccinated with expressed the rabies virus protein
a) on its envelope
b) as part of its capsid
c) on both its envelope and capsid
d) as part of its nucleoprotein
e) from its genome only during the replication in the raccoons

Back 16

e) from its genome only during replication in the raccoons

Front 17

which is incorect regarding felv infection
a) felv is transmitted principally via the saliva during mutual grooming
b) the anemia observed is typically regenerative in nature
c) the felv elisa test detects the presence of p27 antigen in blood of felv infected cats
d) cats with felv induced lymphosarcoma typically do not have ab to the felv focma antigens
e) the felv ifa test detects the presence of felv antigen in the lymphocytes of felv infected cats

Back 17

b) the anemia observed is typically regenerative in nature

Front 18

the diagnostic lab recieves bone marrow sample from a cat suspected of being latently infected with felv. two samples are tested for prseence of felv: 1) dna extracted from the blood ells, and 2) a blood smear made from the bone marrow sample. if this cat is latenlty infected, which tests have potential to dect felv in the latently infected lymphocytes
a) pcr performed on the extracted dna to detect the viral genome
b) fa test to detect viral glycoproteins on the latently infected lymphocytes in the blood smear
c) immunohistochemistry/immunoperoxidase to detect p27 viral capsid proteins in the cytoplasm of the latently infected lymphocytes in the blood smear
d) none of tha bove-
e) all of the above

Back 18

a) Pcr performed on the extracted dna to detect the viral genome

Front 19

which o the following ar true regarding the focma antigen?
a) focma is present on fiv transformed lymphocytes
b) focma is a protein encoded for by fiv
c) cats with lymphosarcoma have circulating ab to focma
d) cats with circulating ab to focma are infected with fiv
e) focma is associated with felv infection and not fiv infection

Back 19

e) focma is associated with felv infection, not fiv infection

Front 20

elisa nand ifa tests are routintely performed on cats to determine if they are infected with felv. the commercially available elisa detects ____ located in the blood, and ifa detects ___ located in/on the ______ of infected cats

Back 20

antigen
antigen
infected lymphocytes

Front 21

what is the prupose of running an ifa in addition to the elisa? what additional info will be proved by the ifa that can be figured out from the elisa alone

Back 21

elisa detects antigenima only whereas ifa can detect viremia. IFA can tell if a positive elisa cat is latently infected or contagious

Front 22

the protein detected by the routinely used felv elisa is

Back 22

p27

Front 23

a cat diagnosed with an alimentary lymphosarcoma is least likely to be
a)vomiting
b) having diarrhea
c) emaciated
d) young, less than 2 years
e) antigenemic and viremic

Back 23

d) young

Front 24

a health cat is present for vaccination. it is antigenemic as determined by the elisa, but non viremic by the IFA test.
a) what sample would you submit for the IFA test
b) what does the IFA test detect in the sample to determine if the cat is viremic
c) explain how a sequestered lesion can result in the cat being antigenemic but non viremic

Back 24

a) air dried blood smear
b) felv antigen on virus infected lymphocytes
c) sequestered lesions produce virus ag but do not infect cells

Front 25

pcr is another test that can diagnose felv infections in cats by detecting the felv in material extracted from circulating lymphocytes. PR dects the presence of felv (rna/dna) in the (cytoplasm/nucleus) of infected lymphocytes

Back 25

DNA
nucleus

Front 26

true or false: a cat with felv induced lymphosarcoma and that is shedding teh virus would be expected to have neutralizing antibodies to the gp 70 viral glycoprotein

Back 26

false

Front 27

true or false: a cat with felv induced lymphosarcoma and that is shedding teh virus would be expected to have circulating antibodies to the focma viral antigen

Back 27

false

Front 28

true or false: a cat with felv induced lymphosarcoma and that is shedding teh virus would be expected to test positive by the ifa test

Back 28

true

Front 29

FOCMA

Back 29

feline oncorna ell membrane associated antigen

Front 30

a vet suspects that a 7 year old tomcat has felv. he has lost weight and is aenmic. if the anemia is associated with felv, describe the type of anemia

Back 30

normocytic, normochromic
non regenerative

Front 31

a vet suspects that a 7 year old tomcat has felv. he has lost weight and is anemic. the vet performs a needle aspirate of the bone marrow, and exams the aspirated cells microscopically. she suspects felv associated lymphosarcoma/leukemia based on the appearance/characteristco of the cells from the aspirate. what might these characterists be? _______
you would expect these tell to test ___ for the FOCMA ag by fluorescent ab test, and for the cat to be ____ for antibeodies to the FOCMA antigen

Back 31

large lymphoblasts
positive
negative

Front 32

the felv vaccine that is routinley administered to cats is a
a) killed virus vacine
b) subunit virus vaccine
c) dna vaccine
d) mod live virus vaccine
e) live virus vaccine

Back 32

b) subunit virus vaccine

Front 33

Which of the following is least correct regarding inclusion bodies. Inclusion bodies:
a. Consist of accumulations of structural viral proteins or complete virions
b. Cannot be seen with a light microscope- only with an EM microscope
c. Caused by herpesvirus are located primarily inside the nucleus
d. Caused by rabies virus are called negri bodies
e. Can be either acidophilic or basophilic

Back 33

cnnot be seen with a light microscope- only with an EM microscope

Front 34

Which of the following is least correct regarding inclusion bodies. Inclusion bodies:
a. Consist of accumulations of structural viral proteins or complete virions
b. Cannot be seen with a light microscope- only with an EM microscope
c. Caused by herpesvirus are located primarily inside the nucleus
d. Caused by rabies virus are called negri bodies
e. Can be either acidophilic or basophilic

Back 34

cnnot be seen with a light microscope- only with an EM microscope

Front 35

which of the following is true? the biologicaly activity or infectivity of the two prion proteins prpsc and prpc will remain unaffected after
a) boiling for 30 minutes
b) fixing with 10% formaldehyde
c) digestion with protease enzyme
d) digestion with nuclease enzyme
e) radiation with ionizing uv light

Back 35

e) radiation with ionizing uv light

Front 36

Perivascular cuffing can be defined as the accumulation of _______ around blood vessels

Back 36

lymphocytes

Front 37

which of the following is not a natural reservoir of the rabies virus in amiera?
a) grey fox
b) coyote
c) raccoon
d) silver haired bat
e) none of the above

Back 37

e) none of the above

Front 38

name a virus that can cause tumors and seizures in cats

Back 38

feline leukemia virus

Front 39

the recombinant vaccinia virus that is used to vaccinate raccoons against rabies is immunogenic. Its immunogenicity is due to the fact that the recombinant virus that the raccoons are vaccinated with expressed the rabies virus protein:
a on its envelope
b as part of its capsid
c on both its envelope and its capsid
d as part of its nucleoprotein
e from its genome only during replication in raccoons

Back 39

from its genome only during replication in raccoons

Front 40

name a disease that can cause pruritis and encephalopathy in sheep

Back 40

scrapie

Front 41

after a bite the rabies virus will replicate locally in the muscle cells before invading the cns. how does it invade the cns? via the _______. Does rabies replicate in the salivary glands? (yes/no)
the routine lab test performed to determine if an animal is rabid is the _____
a skunk that tests negative for rabies by this routine test can still be expceted to transmit rabies virus (true/false)

Back 41

neurons nearby
yes
IFA
False

Front 42

describe the histopathological lesions that helped coin the term spongiform

Back 42

large vacuolization in neurons of brain tissue, with no inflammation

Front 43

a dog is bitten by a rabid skunk. how does rabies virus reach the brain of this dog to infect neurons? Via
a) virus infected circulating lymphocytes that exit blood vessels in teh braine
b) virus infected circulating monocytes that enter the brain by diapedesis
c) viremia- the virus circulations in the blood not associated with blood cells
d) peripheral nerves by axoplasm flow
e) a combo of all the above

Back 43

d) peripheral nerves by axoplasm flow

Front 44

which of the following dogs is least likely to have died of rabies? a dog that is showing the presence of
a) rabies virus in its neurons by the FA test
b) negri bodies in its neurons
c) rabies virus specific IgG circulating in its serum
d) rabies virus specific igg in its CSF
e) rabies virus in the acinar cells of its salviary glands by FA test

Back 44

c) rabies virus specific Igg circulating in its serum

Front 45

from your general knowledge of TSEs, which of the following would you not expct to see in the following animals?
a) long incubation period in a deer diagnosed with CWD
b) trembling, incoordination and paralysis in cattle with BSE
c) encephalitis in a mink that died of TME
d) pruritis in a sheep with scrapie
e) vaculuoated neurons in the cns of a cat that died of feline spongiform encephalopathy

Back 45

c) encaphalitis in a mink with TME

Front 46

how does the abnormal prpsc protein differ from the normal prpc protein. compared to the prpc protein, the prpsc protein contains
a) 3 additional amino accids at its amino terminus
b) 3 additional amino acids at its carboxy terminus
c) 3 less amino acids at its carboxy terminus
d) 3 substituted amino acids
e) no change in amino acids- structurally different with more b-sheets and less a-helices

Back 46

e) no change in amino acids, structurally different with more b sheets and less a-helices

Front 47

which of the following is not a resrvoir of rabies in america?
a) opossum
b) fruit eating bat
c) coyote
d) raccoon
e) vampire bat

Back 47

a) opossum

Front 48

descibe the prodromal form of rabies virus infection and list some specific clinical signs you would expect to see in a cat during this stage

Back 48

theis form is characterized by a change in behavior and the animal may be feverish. behavioral changes in wild animals usually consist of friendly and tame while domestic animals such as nervousness, anxiety and irritation

Front 49

rabies virus will not be dected in the salivary gland of a dog if
a) rabies is absent in the brain of that dog
b) negri bodies are absent in the neurons of the cns of that dog
c) the furious form of rabies is absent in the dog
d) pharyngeal paralysis and excessive salivation is absent in the dog
e) ascending paralysis is absent in the dog

Back 49

a) rabies is absent in the brain of that dog

Front 50

in the Us, CWD is endemic in
a) cattle
b) elk
c) mule deer
d) a and b
e) a and c
f) b and c

Back 50

f) elk and mule deer

Front 51

which of the following samples collected from a rabid horse is least likely to yield laboratory results confirming that the animal died of rabies
a) csf
b) blood sample in heparin
c) salivary gland
d) skin biopsy
e) hippocampus

Back 51

b) blood sample in heparin

Front 52

which of the following is false regarding the agent that causes scrapie in sheep? the agent:
a) causes an encephalitis
b) is transmitted between sheep via the placenta
c) induces no immuno response as a result of the ifnection
d) causes vacuolization of the neurons in the brain
e) will always kill sheep that display clinical signs of infection

Back 52

a) causes an encephalitis

Front 53

what mammal is most resistant to infection with rabies virus?

Back 53

opossum

Front 54

in spite of the fact that the incubation period of rabies can last for years, veterinarians in the us are required to quarentine dogs suspected of having rabies for only 10-14 days in order to determine if the dog (that has bitten someone) is in fact infected. Please explain the discrepancy between the incubation period and the quarentine period

Back 54

compared to the incubation perio,d the development of clinical signs is very short. if the dog has bitten someone they should start to show signs within ten days of the biting

Front 55

of the viruses that can cause cns disease in cats, which virus has been transmitted by the feeding of meat and bone meal derived from british cattle?

Back 55

feline spongiform encephalopathy

Front 56

of the viruses that can cause cns disease in cats, which virus has been associated with lymphomas in the cns and spinal cord?

Back 56

feline leukemia virus

Front 57

PrPsc stands for

Back 57

prion protein scrapie

Front 58

which of the following would be least frequently obserbed in rabies viral infection in cattle?
a) change in voice
b) aggression
c) paralysis
d) salivation
e) pruritis

Back 58

e) pruritis

Front 59

please explain why cats that have serum ab to the FOCMA do not develop FeLV induced lymphosarcoma

Back 59

the Ab to FOCMA lyse the viral infected cell and prevent lymphosarcoma development

Front 60

heavily felv infected cats that are secreting felv in their saliva, will definitely not be
a) antigenemic (Elisa pos)
b) viremic (IFA pos)
c) virus neutralizing ab positive
d) FOCMA ab positive

Back 60

c) VN ab positive

Front 61

Which of the following is incorrect regarding rabies virus inclusion bodies. These IB's
a are observed in the brain cells of all animals that die of rabies virus infection
b are located in the cytoplasm of neurons
c are commonly referred to as negri bodies
d consist of accumulation of viral nucleocapsid proteins
e can easily be observed with both the ligth and electron microscopes

Back 61

a are observed in the brain cells of all animals that die of rabies bodies infection

Front 62

Negri bodies

Back 62

ICIB's, characteristics of rabies infected neurons

Front 63

Which of the following is incorrect regarding rabies virus inclusion bodies. These IB's:
a) are observed in the brain cells of all animals that die of rabies virus infection
b) are located in the cytoplasm of neurons
c) are commonly referred to as negri bodies
d) consist of accumulation of viral nucleocapsid proteins
e) can easily be observed with both the light and electron microscopes

Back 63

a are observed in the brain cells of all animals that die of rabies bodies infection