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90 Cards in this Set

  • Front
  • Back

DNA

Deoxyribonucleic acid, A double stranded polymer of nucleotides

Deoxyrubose sugar

Sugar molecule containing five carbons

Complementary base pairing

Pairing of nitrogenous base


One strand of DNA is opposite of the other


Adenine+thymine and guanine+cytosine

Nitrogenous base

Adenine, thymine, guanine, cytosine

Nucleotides

Molecule consisting of a five carbon sugar with a nitrogenous base attached

Phosphate group

Group of four oxygen atoms surrounding a central phosphorous atom found in backbone of DNA

Antiparallel

Describes 2 adjacent nucleotides running 8n opposite directions relative to one another

Purine

Double ring


Composed of carbon and nitrogen

Pyrimidine

Single ring


Composed of carbon and hydrogen

Phosphodiester Bond

A chemical bond of the kind joining successive sugar molecule in a polynucleotide

1

Hydrogen bonds

2

Phosphodiester bonds

3

Glycosidic bond

4

Adenine

5

Thymine

6

Cytosine

7

Guanine

8

Deoxyribos sugar

9

Phosphate

Wilkins and Franklin studied the structure of DNA using?

X ray diffraction

Wilkins and Franklin studied the structure of DNA using?

X ray diffraction

Template

A single stranded DNa sequence. Acts as guiding pattern to produce a complementary mRNA

Semiconservative

1/2 of parent strand is "conserved" in both new daughter strands

DNA helicase

The enzyme that unwinds double helical DNA

Single stranded binding proteins (SSBs)

A protein that keeps separated strands of DNA apart (prevents annealing)

DNA gyrase

Enzyme that relieves the tension produced by the unwinding of DNA

Replication fork

The region where the enzymes replicating a DNA molecule are bound to untwisted, single stranded DNA

Replication bubble

Where two replication forks are in close proximity to eachother, producing a bubble in replicating DNA

Anneal

Pairing of complementary strands of DNA through hydrogen bonding

RNA primer

A sequence of 10-60 RNA bases that is annealed to a region of single-stranded DNA for the purpose of initating DNA replication

Primase

The enzyme that builds RNA primers

Leading strand

Synthesized continously during DNA replication

Lagging strand

Synthesized in short fragments, which later are joined together

Okazaki fragments

Short fragments of DNA that are a result of the synthesis of the lagging strand

DNA polymerase 1

An enzyme that removes RNA primers and replaces them with the appropriate deoxyribonucleotide

DNA ligase

Enzyme that joins DNA fragments together

DNA polymerase 111

Enzyme that connects new bases to the old bases in DNA template

DNA polymerase 1 and 111

Proofreaders, makes sure there's no mistakes. If mistakes are found, the incorrectly paired nucleotide is removed or excised and the correct one is added.

DNA is in the shape of?

Double helix

Nucleotides are made of?

Sugar, phosphate, and one nitrogen bases

Holds nitrogen bases together?

Hydrogen bonds

Sugar found in DNA

Deoxyribose

Sugar in RNA

Ribose

Chargaffs rule?

Amount of A in DNA is always equal to amount of T

Showed photograph to Watson and Crick

Maurice Wilkins

The difference between hydrogen bonds in A+T and G+C

A+T 2 bonds, C+G 3 bonds

Where do free floating nucleotides come from?

From food we eat

Enzyme unravels DNA

Helicase

Enzymes that attaches free floating nicleotides

DNA polymerase 111+1

Enzyme that connects fragments on lagging strand

DNA ligase

Prevents Annealing

SSBs

Relieves tension on unwound strands

DNA gyrase

RNA that is transcribed from DNA and delivers genetic code to ribosome

mRNA

Twisted ladder structure

Helix

Produced xray to identify DNA structure

Rosalind Franklin

RNA that delivers amino acids to ribosome

tRNA

Process of DNA transferring code to mRNA

Transcription

Terms for sequence of 3 nitrogen bases that form a code

Codon or triplet

Group of three complementary bases on tRNA that pairs with codons on mRNA

Anticodon

3 steps in both transcription and translation

Intation, Elongation, termination

Process of reading mRNA to determine protein structure

Translation

Signals ribosome to attach to mRNA strand

Promoter region ahead of start codon

Ends protein synthesis

Stop codon

Three post transcriptional modifications of mRNA

5' cap, 3' tail and remove introns

Enzyme transcribes mRNA from a DNA template

RNA polymerase

3 results of mutations

Substitution, Addition, and Deletion

Mutation that occurs in the introns of mRNA

Silent mutation

Mutation from result of addition or deletion

Frameshift mutation

Mutation that causes translation to end early

Nonsense mutation

A base substitution that alters a codon and results in the wrong amino acid

Missense mutation

Organelle that is site of protein synthesis

Ribosome

Thymines replacement in RNA

Uracil

mRNA is made during? And where?

Transcription, in the nucleus

DNA is located in?

Nucleus

Process that converts DNA into mRNA

Transcription

Makes up ribosomes

rRNA

Amino acids held together by?

Peptide bond

Transcription takes place in?

Nucleus

tRNA is uses what to match to mRNA?

Anticodons

Proteins made at?

Ribosome

Where does second step of protein synthesis occurs?

Cytoplasm

What attaches amino acids into a chain?

tRNA

tRNA is found in?

Cytoplasm

Exons

Coding

Introns

Non coding

Point mutation

Only one base is affected

Silent mutation

When nucleotide changes, resulting amino acid is still the same

Frameshift mutation

When base is added or removed

Nonsense mutation

When it's terminated early

Missense Mutation

Substitution and incorrect amino acid is placed