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253 Cards in this Set

  • Front
  • Back

Types of water in soil

Hygroscopic , Combined, Gravitational, Capillary

____________ water is held very tightly around soil particles by adhesive forces

Hygroscopic

Meaning of hygroscopic water

water held very tightly around soil particles by adhesive forces

Meaning of combined water

Water present in form of hydrated oxides of Al,.Si, etc.

What is combined water? How much of it available to plants?

Hygroscopic and combined. Not available to plants

Meaning of gravitational water

Due to gravitational force, some amount of water goes beyond through the large pores between soil particles, goes beyond the reach of roots of plants and reaches the water table

Which type of water is available to plants?

Capillary water

What is capillary water?

In between small, colloidal soil particles, very small spaces (capillaries) are present. The water in this spaces is known as capillary water available for absorption.

Diffusion is obvious in ____________. Give reason-

Gases and liquids due to high KE

Define Simple Diffusion

Movement of ions, atoms, or molecules of solutes, liquids or gases from region of hugher concentration to region of lower concentration till equilibrium is reached.

Which of Diffusion, facilitated diffusion and active transport is not dependent on living system?

Diffusion

In diffusion, particles move in a ____________ fashion.

Random

Comment on probability of diffusion w.r.t. solids.

Diffusion in solids is more likely than diffusion of solids

Why is diffusion very very important in plants?

It is the only means for gaseous movement within body

Factors affecting diffusion rates

1. Concentration gradient


2. Permeability of separating membrane


3. Temperature


4. Pressure


5. Size of substances


Diffusion pressure is (cause/ result) of diffusion

Cause

Define diffusion pressure

Potential ability of solid liquid or gad to diffuse from an area of its greater concentration to an area of lesser concentration

DP proportional to -

Number of diffusing molecules

Effects of adding solutes to water

DP reduces

What is DP of pure water?

1236 (Maximum)

What is facilitated diffusion?

Selective transport of large molecules across cell membrane assisted by special proteins (porins) without expenditure of energy

Water soluble substances are transported across membranes by ___________ ___________ aquaporins.

8 different

Diffusion of a substance across cell membrane primarily depends on its _______________.

Solubility of lipids

Which substances diffuse through cell membrane faster?

Fat soluble/ Hydrophobic

Which substances find difficult to diffuse through cell membrane ?

Substances that have a hydrophilic moeity

Porins facilitate diffusion by setting up a concentration gradient - True or False giving reasons

False - They do set up a gradient, a gradient should already be present

How do porins help in transport?

The proteins form channels in the membrane for molecules to pass through. Some channels are always open others can be controlled.

Give types of passive transport with meaning

Symport - 2 molecules in same direction


Anti port - 2 molecules in Opp. Direction


Uniport - 1 molecule membrane independently

Factors increasing active transport

Higher rate of respiration - more ATP - more salt accumulation

Factors reducing active transport

Respiratory inhibitors, Low O2 content

What the proteins used for active transport known as?

Pumps

Transport from lower to higher concentration to lower concentration also known as -

Uphill transport

Compare diffusion, facilitated diffusion and active transport w.r.t. to above points

Water has ________________ so it can move up by capillary action.

High surface tension

Water has ________________ which enables to form a continuos stream of water.

High tensile strength

Water has high ____________, ____________, ____________, hence it acts as temperature stabilizer.

Specific heat, heat of fusion, heat of vaporization

Water has high Specific heat, heat of fusion, heat of vaporization so it can act as _____________.

Temperature stabilizer

In what activities is water required w.r.t. reproduction and gaseous exchange? (4)

Movement of gametes, stomatal movements, dehiscence of fruits, germination of seeds

How does water help aquatic plants?

Transparent, so light passes - photosynthesis

Percentage of water in


1. Watermelon


2. Most herbaceous plants

1. 92%


2. 10-15% of fresh weight as dry matter

Is a seed actually dry? Give reason

No, it has water. Otherwise, it wouldn't be alive and respiring

Give amount of absorption of water by


1. A mature corn plant


2. Mustard plant

1. About 5 L/ day


2. = Body weight / 5 hr

Water is a limiting factor for _____________ and ____________ in both ____________ and ___________ environments.

Plant growth, productivity, agricultural, natural

Examples of membranes


1. Freely Permeable


2. Semi Permeable


3. Selectively Permeable


4. Impermeable

1. Cell wall


2. Parchment paper, Egg membrane


3. Cell membrane, tonoplast


4. Suberin in cork and endodermis, cuticle

What do the following membranes allow to pass?


1. Freely Permeable


2. Semi Permeable


3. Selectively Permeable


4. Impermeable

1. Solvent, solute


2. Solvent


3. Solvent, some selected solutes


4. None

Water always goes from _________ to ___________.

Hypotonic to hypertonic

Concentration of solution is given by _______________. Its unit is ______________.

Osmolarity, milliosmoles/litre

Formula of osmolarity

Moles of solute / volume of solvent in litres

Define osmosis

Diffusion of water or solvent from a solution of lower concentration to a solution of higher concentration or from pure solvent to solution through semi-permeable membrane

Type of surrounding solution for following condition


1. Turgid


2. Flaccid


3. Plasmolysed

1. Hypotonic


2. Isotonic


3. Hypertonic

What happens when cell is kept in -


1. Hypotonic


2. Hypertonic


3. Isotonic

1. Endosmosis - Turgid


2. Exosmosis - Plasmolysed


3. No net flow - Equal in and out - Flaccid

In plant cells, the _____________ contributes to solute potential of cell.

Vacuolar sap

In plant cells, the vacuolar sap contributes to ___________ of cell.

solute potential

At equilibrium, both cell and surrounding should have same ______________.

Water potential

Which is the most accepted mechanism used for Translocation of sugar from source to sink? Who put it forward?

Pressure/ mass flow hypothesis by E. Munch

Process of mass flow hypothesis

Glucose - Sucrose - Companion cells - Sieve tube (active transport) - hypertonic - water from xylem - OP increase - decrease at sink - active transport - glucose - energy use - water to xylem

Loading of phloem sets up a __________________ that facilitates _______________ in phloem.

Water potential gradient, mass movement

Which experiment is used to identify the tissues through which food is transported?

Girdling

What is done in girdling?

On the trunk of a tree, ring of bark upto a depth of phloem layer is removed.

Girdling shows that _________ is the tissue responsible for Translocation of food and that transport takes place in _______ direction.

Phloem, one


(Note: in this experiment, it shows unidirectional, answer is not wrong)

Since the source-sink relationship is ________, (depending on _____________) direction of movement in phloem is bidirectional.

Variable, season or plant's needs

Movement in phloem is _________ directional.

Bi

Compare C3 and C4 plants in terms of efficiency of carbon fixation into sugar

C4 are twice efficient than C3

Compare C3 and C4 plants in terms of water loss during photosynthesis (CO2 fixation)

C4 loses only half as much water as C3 for same amount of CO2

Environmental factors affecting transpiration

Temperature, light, humidity, wind speed

Effect of humidity on transpiration

More humidity less transpiration

Least transpiration occurs at _____________ humidity.

100% relative

Plant factors affecting transpiration

Number and distribution of stomata, percent of stomata open, water status of plant , canopy structure, etc.

Transpiration driven ascent of xylem sap depends mainly of following properties of water:

Cohesion, Adhesion, Surface Tension, Tensile Strength, Capillarity

Cohesion, Adhesion, Surface Tension give water high __________ and high __________.

Tensile strength, Capillarity

Measurements reveal ghat forces generated by transpiration can create pressures sufficient to lift xylem sized column of water over ___________ high.

130 m

Who called transpiration necessary evil and unavoidable evil respectively?

Curtis, Steward

Mesophyll with intercellular spaces and stomata are essentially meant for ___________, but the _____________ is unavoidably responsible for transpiration.

Gaseous exchange, leaf anatomy

Benefits of transpiration (key words)

1. Cooling effect


2. Mineral Nutrition


3. Water movement


4. Optimum turgidity

Which benefit of transpiration has exception? The exception is related to _____________.

Optimum turgidity, succulent plants

Transpiration cools surface by ____________ degrees by ___________.

10 to 15, evaporative cooling

Transpiration supplies water for __________________.

Photosynthesis

Transpiration maintains _________________ of plants by keeping cells turgid.

Shape and structure

Each gram of water adsorbs ______________ heat from leaf and its environment.

580 cal

Transpiration is important for miner Nutrition because -

Minerals are transported through transpiration stream

Transpiration provides an efficient means of ____________ and ___________ of salts absorbed by roots.

Transport and distribution

Water absorbed is translocated through xylem vessels in a ______________.

Continuous water column

Transpiration maintains ___________ in cells.

Optimum degree of turgidity

Plants cells function best when there is some water __________ (deficit/excess)

Deficit

How much amount of water absorbed is lost through transpiration? What is the effect?

98%, large amount of energy wasted

Transpiration causes water deficit and plants suffer from ___________ due to ______________.

Injury, dessication

If amount of water lost exceeds the amount of water absorbed, then it causes ____________.

Wilting of leaves

What cannot be explained by transpiration?

Movement of food

Succulent plants store water in _______________.

Special water storage tissues

Stomata movement are brought by changes in ____________.

Volume and shape of guard cells

Guard cells absorb water from ____________.

Subsidiary cells

What happens to the shape of guard cells when they absorb water?

Pressure on walls causes thin outer wall to bulge out and inner walls to crescent shape

Who proposed the proton transport concept? It explains ____________.

Levitt, mechanism of opening and closing of stomata

According to proton transport concept, the opening and closing of stomata takes place as a result of ______________.

Active transport into and out of guard cells

Which 2 major compounds are involved in opening and closing of stomata?

Starch, CO2

During day, starch is converted into __________ in ___________ which in turn dissociates into _________.

Malic acid, cytoplasm of guard cells, H+ and malate ions

H+ ions given out of cells and ___________ from ________ enter into guard cells.

K+ ions, subsidiary cells

The intake of K+ ions is balanced by intake of _____. This raises __________ of guard cells.

Cl- ions, osmotic pressure

Answer w.r.t. mechanism of opening and closing of stomata:


At night photosynthetic activity ceases. What is the effect? (Minor level)

CO2 concentration increases


pH becomes acidic 5.0

In presence of CO2, an inhibitor hormone _____________ functions and inhibits Uptake of K+ and Cl- ions by -

Abscissic acid (ABA)


1.Changing diffusion


2. Changing permeability of guard cells

Who proposed Starch sugar hypothesis? It explains ____________.

Steward, mechanism of opening and closing of stomata

Explain starch sugar hypothesis for opening of stomata

Day - chloroplasts in guard cells - photosynthesis - sugar - OP increase - Endosmosis

Explain starch sugar hypothesis for closing of stomata

Night - sugar to starch - increase TP - exosmosis

Mention the parts of stomatal apparatus with numbers

1. 2 Guard cells


2. 1 Stoma


3. Many accessory or subsidiary cells

Guard cells are ______________. What is the speciality of these cells w.r.t. other cells of the tissue type?

Modified Epidermal cells, presence if chloroplasts

Shape of guard cells

Dicot - kidney shaped


Monocot - Dumbbell shaped

How many nuclei do guard cells have? How is the cytoplasm?

1, peripheral granular

Comment on walls of guard cells

1. Inner - Thick, elastic


2. Outer - Thin, elastic, Permeable

Inner wall of guard cells is thick due to _____________.

Presence of secondary wall layer

Opening of stoma is also aided due to _________________ of guard cells

Radial orientation of cellulose microfibrils in cell walls

What is a stoma?

Elliptical pore formed due to specific arrangement of guard cells

What are accessory cells?

Cells immediately surrounding guard cells

____________ are reservoirs of K+ and Cl- ions.

Accessory cells

Subsidiary cells are reservoirs of ___________.

K+ and Cl- ions

Which gases are exchanged through stomata?

CO2 and O2


(Note: not H2O because it is only diffused out - not involved in exchange)

Which gas only diffuses out through stoma?

Water vapours

How much percent of water absorbed is utilized by plants?

2%

Define transpiration

Evaporative loss of water in form of water vapour by aerial parts of plants

Mention types of transpiration with percentage transpiration through each

1. Cuticular - 8-10%


2. Lenticular - 0.1%


3. Stomatal - 80-90%

What is cuticle?

Cuticle is waxy coating on epidermis of leaves

Thickness of cuticle varies from plant to plant - True or False

True

Function of cuticle

To Check transpiration

Actual cuticular transpiration takes place through _______

Cracks in cuticle or by diffusion through thin areas of cuticle

Factor affecting Rate of cuticular. Give one application

It is inversely proportional to thickness of cuticle.


In xerophytes, cuticle is thicker to decrease water loss

Lenticular respiration occurs in -

Older bark


Pericarp of woody fruits


Pneumatophores

Give the types of leaves based on location of stomata

Stomata connect -

Intercellular spaces between spongy tissues to atmosphere

Diffusion can account for only _____________ movement of molecules.

Short distance

Movement of molecules across a typical plant cells (about _________) takes approximately ________.

50 microns, 2.5 sec

Water, minerals and food are generally moved by ______________ system.

Mass or bulk flow

What is mass flow?

Mass flow is the movement of substances in bulk or en masse from one point to another as a result of pressure differences between the two points

What is characteristic of mass flow?

Substances in solution or in suspension are swept along at same pace

Bulk flow can be achieved by ___________ or _________.

Positive hydrostatic pressure, negative hydrostatic pressure

Give one example each of - Positive hydrostatic pressure, negative hydrostatic pressure

1. Garden hose


2. Suction through straw

Define Translocation

The bulk Movement of substances through the conducting or vascular tissues of plants is called Translocation

Colour of root hair

Colourless

Layers of cell wall of root hair

Outer pectin, inner cellulose (OPIC)

Pathway of water was given by ____________.

E. Munch

According to E. Munch, what is the path of water?

Soil - Epidermis - Cortex - Endodermis - Pericycle - Xylem

What are the main pathways of water?

1. Apoplast


2. Symplast


3. Transmembrane

Apoplastic movement of water takes place through -

Cell walls and intercellular spaces

____________ pathway does not involve crossing cell membrane.

Apoplast

Choose correct option in each w.r.t. to apoplast -


1. Osmotic/ Non-osmotic


2. Dependent/ Independent of gradient


3. Due to absorption/ imbibition

1. Non-Osmotic


2. Dependent


3. Imbibition

Apoplast is continuous movement throughout the transport - True or False giving reason

False - Apoplast is continuous movement throughout the plant, except the casparian strips

Which pathway of water is faster and why?

Apoplast - because it provides no barrier to water movement

Xylem and phloem are parts of _____________ pathway. Why?

Apoplast - they are non living conduits

What is symplastic pathway?

Symplastic system is the system of interconnected protoplast through plasmodesmata

What is symplast?

Neighbouring cells are connected through cytoplasmic strands that extend through plasmodesmata and form cytoplasmic network call symplast.

Symplastic movement is aided by ______________.

Cytoplasmic streaming

Which layer of root cells is impervious to water and why?

Endodermis. - band of suberised matrix called casparian strips

Movement of water through root layers is ultimately ___________ in __________.

Symplastic, endodermis

Transmembrane pathway of water is through ____________.

All layers of cell (including tonoplast)

How are mycorrhiza useful for plants in absorption of water?

Very large surface are

Give example of obligate association of plant with mycorrhiza

Pinus seeds cannot germinate without mycorrhiza

Factors affecting water absorption

1. Availability of soil water


2. Concentration of soil solution


3. Soil aeration


4. Soil temperature


5. Osmotic pressure of root hair


6. Root anatomy


7. Transpiration

How does availability of soil water affect absorption?

Decrease in soil water below the permanent wilting percentage causes considerable decrease in absorption of water.

How does concentration of soil solution affect absorption?

Increased concentration of soil due to presence of salts inhibits

What is physiologically dry soil?

Soil in which water is concentrated. Having high osmotic pressure. Here water is physically present, but not available for absorption

How does soil aeration affect absorption?

Low O2 and high CO2 retards growth and disturbs metabolism. Retards rate of absorption

Optimum range of soil temperature is ______________ for maximum water absorption.

20℃ to 30℃

How does osmotic pressure of root hair affect absorption?

More OP more rate of absorption

How does root anatomy affect absorption?

Less root hair, thick walled cortical cells, casparian strips reduce rate of absorption

How does transpiration affect absorption?

High rate of transpiration more absorption rate

What are the two theories explaining why root cells are hypertonic?

1. Active Osmotic Theory


2. Passive Transpiration Theory


(AOPT)

According to active Osmotic Theory, root hair and cortical cells are hypertonic due to -

Metabolic activities in the living cells of root utility ATP

In active osmotic theory, after absorption water is transported to next cells due to __________.

DPD

According to active Osmotic Theory, root cells alternately become _______________.

Flaccid and turgid

According to passive transpiration theory, what is major factor for absorption of water?

Suction or cohesive pull developed by the leaves due to transpiration develops water deficit and draws water from the petiole which pulls water from the stem

According to which theory of absorption, metabolic energy is not used?

Passive transpiration theory

Role of root in absorption according to passive transpiration theory

Only ad physical absorbing organ providing surface area for absorption

Role of root in absorption according to passive transpiration theory

Only ad physical absorbing organ providing surface area for absorption

One atmospheric pressure raises a column of water up to _______ m or ______ feet

10.3 m, 34 feet

Height of


1. Californian Sequoia plant


2. Australian Eucalyptus plant

1. 111.6 m or 366.2 feet


2. 114.4 m or 374 feet

In a well watered potted tomato plant, cut the stem close to root. What will you observe?

Exudation/ bleeding of xylem sap from stump

How can we measure the rate of exudation from cut stump? What else can be determined?

Fix a rubber tube to cut stem as a sleeve and collect, composition of exudates

Root pressure is a (positive/ negative) pressure, responsible for -

Positive, pushing up water to small heights in stem

Who explained Root pressure?

Priestly

What was Priestly's explanation of root pressure?

Priestly explained that a sort of hydrostatic pressure develops in the root due to accumulation of absorbed water

What is guttation?

Water loss in its Liquid phase through intact plant parts is known as guttation

Guttation is observed in -

Grass blades, fern leaflets, leaves of many herbaceous plants

Excess water in tips of Grass blades, fern leaflets, leaves of many herbaceous plants etc collects in form of ____________ around special openings called _____________ of __________.

Droplets, hydathodes, veins

Exuded water from guttation often contains __________. What is the further consequence?

Calcium salts, water evaporates off, white crust remains on surface

Hydathodes are also known as ___________.

Chalk glands

2 facts confirming root pressure:

1. Living cells are essential in the root for root pressure to develop


2. Oxygen supply effects root pressure

What is magnitude of root pressure?

Less than 2 atm

How is root pressure measured?

By manometer sealed over cut stump

Root pressure is sufficient only for ____________.

Herbs and shrubs

Root pressure is not applicable to plants taller than __________

20 m or 68 feet

Comment on root pressure in tall connifers and other gymnosperms

Absent

Root pressure is absent in _________.

Tallest plants like conifers and other gymnosperms

Season dependence of root pressure. So Why root root pressure is not applicable?

During summer root pressure is lowest when transpiration rate is very high, wheras during spring it is highest when transpiration rate is low. Actually in summer aerial parts of plants need maximum water

What cannot be explained by root pressure theory?

Root pressure theory cannot explain ascent of sap through xylem elements which is dead lignified tissue

Capillarity theory is a (physical/ living) theory

Physical

Who gave Capillarity theory?

Boehm

According to Boehm water rises in narrow tube by -

Capillarity develop because of force of surface tension just as oil rises in the wick of an oil lamp

According to Capillarity theory, water continues to rise until -

Forces of Adhesion and cohesion are balanced by downward force of gravity

In a 1 micron Capillary, water rises to a height of ___________, only due to ____________.

29 m, resistance to flow of water

Demerits of Capillarity theory

1. Distance to which water can rise is limited


2. Tube must be open, hollow and open at both ends. In gymnosperms and pteridophytes, xylem is vessel less and tracheids have cross walls at ends


3. Requires free surface, but xylem not in direct contact with water


4. Requires uniform diameter, but not so in xylem


5. Thinner capillary, more rise distance. Opposite in xylem.

Who proposed cohesion theory?

Dixon and Jolly

Magnitude of cohesive force of water ___________

350 atms

Cell with shrunken protoplasm is called ____________.

Plasmolysed cell

Water is first lost from ___________ and then from __________.

Cytoplasm, vacuole

When Plasmolysed cell is placed in hypotonic solution, it reabsorbs by _______________, its protoplast resumes original shape. This is known as ___________.

Endosmosis, deplasmolysis

What is osmotic pressure?

Pressure applied to stop osmosis or the pressure needed to prevent passage of pure water through semi-permeable membrane

OP is proportional to -

Solute molecules in given amount of solvent

Compare OP of hypertonic and hypotonic solution

OP of Hypertonic more than OP of hypotonic

Give relationship between osmotic pressure and osmotic potential

Numerically equal, opposite in sign. Osmotic pressure is positive pressure applied, osmotic potential is negative

Give alternate name and symbol for osmotic potential

Solute potential - Psi(s)

Define turgor pressure

The hydrostatic pressure that develops due to endosmosis inside plant cell

Define pressure potential

The pressure exerted by protoplasts due to entry of water against rigid walls

______________ is ultimately responsible for enlargement and extension growth of cells

Turgor pressure

After endosmosis, why doesn't cell rupture?

Rigidity of cell wall

What is wall pressure?

Protoplasts exert pressure potential on wall. Cell wall is rigid and develops an equal and opposite pressure on cell contents known as WP

Relate TP, WP and OP

In fully turgid cell, TP=WP=OP

Give full form of DPD, along with alternate names and abbreviations

Diffusion pressure deficit


Suction force (SF)


Suction pressure (SP)

What is DPD?

Difference between DP of pure water (1236) and that of solution

DPD is proportional to

Solute

Give formulae of DPD

DPD = DP (pure water : 1236) - DP (solution) = OP - TP (WP)

DPD in a fully turgid cell is _______

Zero

When DPD =0, what happens?

Endosmosis stops

Water potential is denoted by _______ and is measured in _______.

Psi(w), pressure units like pascals

Pressure potential is denoted by

Psi (p)

What is value of water potential at atmospheric pressure?

Psi(w)=Psi(s)

What is water potential?

Chemical potential of water or potential energy of water

Water potential is proportional to

Solvent

Give formalae for water potential

Psi(w) = Psi (s) + Psi(p) = - OP + TP = - DPD

Define solute potential

Magnitude of lowering of water potential due to dissolution of solute is called solute potential

If pressure greater than atmospheric pressure is applied to solution, it's water potential (increases/decreases)

Increases

State whether positive or negative:


1. Pressure potential


2. Solute potential

1. Usually positive


2. Always negative

Comment on movement of water w.r.t. to following:


1. Concentration


2. OP


3. Psi(s)


4. DPD


5. Psi(w)

1. Po to per


2. Less to more


3. More to less (less negative to more negative)


4. Less to more


5. More to less (less negative to more negative)

Which are the first and second physical processes to occur?

Imbibition and Diffusion respectively

What is imbibition?

Adsorption of water by hydrophilic compounds

What happens in Adsorption?

Water gets tightly adsorbed on surface of compounds without forming a solution. So, compounds show swelling

Meaning of imbibant and imbibant

1. Imbibant - substance/solid/colloid which adsorbs water


2. Imbibate - adsorbed liquid

What is heat of wetting?

Water molecule get highly adsorbed and become immobilized and lose most of kinetic energy in form of heat

After imbibition, imbibant increases in ___________.

Volume

Pre-requisites for imbibition

1. Water potential gradient


2. Affinity


Between imbibant and liquid

Examples of imbibants

Proteins, cellulose, pectic compounds, starch, gum, dry seeds

Why is glucose converted into Sucrose for transportation?

Sucrose is osmotically active

Give following values for pure water and mention whether maximum or minimum


1. DP


2. OP


3. DPD


4. Psi (w)

1. 1236 (Max)


2. 0 (Min)


3. 0 (Min)


4. 0 (Max)

Give stages of plasmolysis with description

1. Limiting - Normal Cell


2. Incipient - Protoplasm withdraws from corners


3. Evident/ Existing/ Obvious - Complete detachment from cell membrane


(LIE)

Magnitude of ___________ of water is 350 atms

Cohesive force

Water has great cohesive force due to ___________.

Hydrogen bonds

Cohesive and adhesive properties developed form ____________ in xylem.

Unbroken continuous water column

How is a continuous column of water maintained in xylem?

Xylem vessels are short,wide and without end walls. They are placed one above the other and form a tubular structure which extends from the root to the top of the plant.


OR


Adhesive and cohesive forces, continuous anastomizing network of xylem elements

Transpiration pull develop a (positive/negative) pressure or ___________ of about ______ atm in xylem sap.

Negative, tension, 20

H-bond is very (weak/strong).

Weak

Although, H-bond is very weak, how does is account for strong cohesion?

When present in large numbers as in water, a very strong mutual cohesive force is developed

According to cohesion theory, what causes pulling force for water?

Water lost from mesophyll due to transpiration - increase OP - tension - pulling force

What is the supposed demerit of cohesive theory?

Due to variations in temperature/ atmospheric pressure, air bubbles may break continuity of water column

How do xylem vessels allow collateral flow?

Tracheids and vessels are pitted. These pits connect adjacent cells with one another and allow collateral water flow

Who showed that under normal conditions, no air bubbles are present in xylem even in last stage of wilting?

Renner and Bode

What was Renner and Bode's observation?

Under normal conditions, no air bubbles are present in xylem even in last stage of wilting