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24 Cards in this Set
- Front
- Back
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Routine h&e staining |
Rehydrate ↓ alcohol Harris hematoxylin 5mins Dip in water 1% acid alcohol 10-30 sec Rinse 5% eosin 5 mins Wash Dehydrate |
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Blue to black due to hematoxylin |
Nuclei |
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Dark blue |
Karyosome |
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Pink |
Cytoplasm |
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Bright orange red |
RBC Eosinophil keratin |
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Blue |
Cement line of bones |
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Deep pink |
Decalcified bone |
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Light pink |
Collagen |
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Aluminu. Ripening agt For progressive and regressive Natural ripening Sodium iodate for faster ripening but shorten lifespan of erhlich Not ideal for RFS and mucopolysacc For catilage and bones |
Aluminum Hematoxylin |
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Most commonly used RA mercuric chloride Good nuclear stain + Glacial acetic acid Color: blue to purple |
Harris hematoxylin |
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RA Alcoholic iodine |
Coles hematoxylin |
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Same as ehrich for nuclear staining RA sodium iodate |
Mayers hematoxylin |
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Kinds of aluminum hematoxylin |
Ehrlich Harris Coles Mayers |
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RA made of ironFor regressive staining only |
Iron hematoxylin |
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Std iron hematoxylin For demonstration of CT |
Weigerts |
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Iron oxidizing agt For demonstration of nuclear and cytoplasmic inclusion |
Heidenhans |
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Best by natural ripening When rippened turns to reddish brown- purple |
Phosphotungstic |
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Used for study of spermatogenesis |
Copper hematoxylin |
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Kinds of iron hematoxylin |
Weigerts Heidenhans Phosphotungstic Cipper hematoxylin |
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Counterstain for h&e technique Reddish to orange color |
Eosin |
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Most commonly used eosin |
Eosin Y/ yellow |
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Color red aka erythrosin B |
Eosin B |
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Eosin that is Alcohol soluble aka ethyl eosin |
Eosin S |
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Kinds of eosin |
Eosin Y,B, and S |
