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15 Cards in this Set
- Front
- Back
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Purpose of staining |
Observe and appreciate the appearance Differentiate one organism to another Helps in identification of organism and its specia structure |
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Types of stain |
Simple Indirect or negative Special Differential |
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This stains the background |
Negative staining |
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Example of negative staining |
India Ink Nigrossing Method |
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Example of capsular stain |
HGAWTM Hiss Gins Anthonys Welch Capsular Tylers Muirs |
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Example of Metachromatic Granules stain |
NALL Neisser Alberts LAMB Ljubinsky |
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LAMB |
Loefflers Alkaline Methylene Blue |
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Process of Differential Stain |
PMDS Primary stain Mordant Decolorizer Secondary stain |
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Gram Stain |
Crystal Violet Iodine 95% Alcohol Safranin |
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Result of Gram Stain |
Gram (+) - purple Gram (-) - Red |
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General Rule of Staining |
All cocci are gram + except NMBV All bacilli are gram - except MCCBLLE |
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Reasons why gram + becomes gram - |
Smear too thick Overdecolorization Too old culture Mixed culture Improper washings |
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Acid fast staining method |
Hot method - Ziehl Neelsen Cold method - Kinyouns |
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Differentiate hot method to cold method |
Primary stain: both carbol fuchsin Mordant: heat | phenol or tergitol Mordant: both 3% acid alcohol Counterstain: methylene blue | malachite green |
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Results of acid fast |
AFO - Red NAFO - blue or green |
