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44 Cards in this Set
- Front
- Back
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Which of the following stain blue with the H&E stain?
a) cytoplasm b) collagen fibers c) nucleus d) elastic fibers e) decalcified bone matrix |
c) nucleus
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What are Sudan stains primarily used for?
a) blood b) fat c) nervous tissue d) elastic fibers e) decalcified bone matrix |
b) fat (or lipids)
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What is the Mucicarmine stain primarily used for?
a) blood b) fat c) nervous tissue d) elastic fibers e) epithelial mucin |
e) epithelial mucin
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What color does the epithelial mucin take up in the Mucicarmine stain?
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deep red
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What is Wright's stain primarily used for?
a) blood b) fat c) nervous tissue d) elastic fibers e) decalcified bone matrix |
a) blood
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What color will the red blood cells stain for Wright's stain?
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pink
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What colors do elastic fibers stain with Verhoeff elastic stain?
a) red/orange b) pink/red c) purple/red d) blue/black e) green/blue |
d) blue/black
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What colors to collagen fibers stain with Verhoeff elastic stain?
a) red/orange b) pink/red c) purple/red d) blue/black e) green/blue |
b) pink/red
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During preparation of routine H&E slides, how is tissue preserved?
a) fixation b) embedding in paraffin c) staining d) slicing e) dehydration |
a) fixation
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During the preparation of a routine H&E slide, what step occurs after the tissue is preserved?
a) fixation b) embedding in paraffin c) staining d) slicing e) dehydration |
e) dehydration
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During preparation of a routine H&E slide, what allows the tissue to be visualized?
a) fixation b) embedding in paraffin c) staining d) slicing e) dehydration |
c) staining
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Which of the following would be best suited to view reticular fibers?
a) Wright's stain b) H&E stain c) Sudan stain d) silver impregnation e) Masson's trichrome stain |
d) silver impregnation
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Which of the following would be best suited to view lipids?
a) Wright's stain b) H&E stain c) Sudan stain d) silver impregnation e) Masson's trichrome stain |
c) Sudan stain
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Put in order the steps of processing and staining from start to finish:
a) dehydration b) staining c) fixation d) embedding in paraffin e) rehydration f) slicing (with microtome) |
c) fixation
a) dehydration d) embedding in paraffin f) slicing (with microtome) e) rehydration b) staining a) dehydration |
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During the preparation of a routine H&E slide, what allows the tissue to hold its form?
a) fixation b) embedding in paraffin c) staining d) slicing e) dehydration |
b) embedding in paraffin
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During the preparation of a routine H&E slide, what step occurs after the tissue is embedded in paraffin?
a) fixation b) embedding in paraffin c) staining d) slicing e) dehydration |
d) slicing
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During the preparation of a routine H&E slide, what step occurs after staining?
a) fixation b) embedding in paraffin c) staining d) slicing e) dehydration |
e) dehydration
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Which of the following is an acid dye?
a) Azure II b) Toluidine blue c) Methylene blue d) Eosin e) Thionine |
d) Eosin
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What type of charged particles are stained by eosin?
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negative
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Which of the following would be best suited for peripheral blood smears?
a) Wright's stain b) H&E stain c) Sudan stain d) silver impregnation e) Masson's trichrome stain |
a) Wright's stain
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Which of the following stains is used for routine histological examination?
a) Wright's stain b) H&E stain c) Sudan stain d) silver impregnation e) Masson's trichrome stain |
b) H&E stain
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What is the Prussian blue stain used for?
a) sodium b) potassium c) calcium d) copper e) iron |
e) iron
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What color does hematoxylin stain structures?
a) orange b) blue c) pink d) red e) green |
b) blue
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Which of the following would be best suited to differentiate between collagen fibers and other fibers?
a) Wright's stain b) H&E stain c) Sudan stain d) silver impregnation e) Masson's trichrome stain |
e) Masson's trichrome stain
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What control tissue is used for the PAS stain?
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kidney
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What does PAS stain?
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glycogen
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Which hematoxylin is used to stain cytology specimen?
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Gill's
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What type of hematoxylin is considered an iron hematoxylin?
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Weigert
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What are the different types of hematoxylin?
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-Harris
-Delafield -Mayer -Ehrlich -Gill -Weigert |
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What reagent can be substituted for hematoxylin in routine H&E staining?
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celestine blue
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Which type of hematoxylin is generally used for routine H&E staining?
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Harris
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Which hematoxylins oxidize naturally?
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-Delafield
-Ehrlich |
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Which hematoxylins are progressive stains?
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-Mayer
-Weigert |
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For aluminum hematoxylins, what reagent is considered the mordant?
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ammonium aluminum sulfate
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What reagent is the mordant with Weigert hematoxylin?
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ferric chloride
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Which stain is used to differentiate cells in bone marrow?
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Wright's stain
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What nucleic acid stain is used to differentiate RNA and DNA?
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methyl green-pyronin Y
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What nucleic acid stain is used to stain DNA only?
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Feulgen reaction
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What does Mayer mucicarmine stain?
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-mucin
-encapsulated fungi -cryptococcus |
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What does the alcian blue stain? What structure?
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-sugar
-goblet cells |
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What are the name of the three stains that will stain amyloid?
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-congo red
-crystal violet -thioflavin T |
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What microscope is needed for the thioflavin T stain?
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fluorescent
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What color does amyloid appear under a phase-contrast microscope when stained with Congo red?
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apple green
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What color is amyloid stained when using the Crystal Violet stain?
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-light purple
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