Scrappy Chixs Lab 2

Front 1

Mannitol Salt agar is both ________ and ____________.

Back 1

Selective and Differential media. Selective is based on the ability to grow in the presence of 7.5% NaCl. (Halotolerence). The media is differential in that it indicates the use of mannitol by changing the phenol red indicator from red to yellow as the pH drops.

Front 2

Halotolerent organisms include the ________ and _______.

Back 2

Staphylococci and micrococci

Front 3

Mannitol

Back 3

a sugar alcohol

Front 4

Mannitol Salt Agar

Back 4

Salt resistant - ( upper left side of plate), Salt resistant mannitol + (yellow section on plate), Salt sensitive, unknown mannitol reaction ( lower )

Front 5

Eosin Methylene Blue Agar is both _________ and _________.

Back 5

Selective and differental

Front 6

Eosin Methylene Blue Agar is selective against?

Back 6

Gram positive organisms because of the eosin Y and methylene blue dyes in the media.

Front 7

Why is Eosin Methylene Blue Agar differential?

Back 7

It is differentail because it seperates the Gram negative organisms into those that ferment lactose and sucrose from those that cannot.

Front 8

As the degree of fermentation increases the fermentors colors range from _________to______to dark_______ or even ________ with a metallic green sheen.

Back 8

pink, red, purple or even black.

Front 9

What is an example of a fast fermentor in Eosin Methylene Blue Agar?

Back 9

Escherichia coli

Front 10

Give an example of a slow fermenter in Eosin Methylene Blue Agar.

Back 10

Enterobacter aerogenes

Front 11

Staphylococcus epidermidis

(Eosin Methylene Blue Agar)

Back 11

Gram positive - inhibited - no growth

Front 12

Eosin Methylene Blue Agar

Back 12

Escherichia coli fast fermenter ( upper left corner)

Enterobacter aeogenes slow fermenter ( lower right )

Staphylococcus epidermidis - gram postive- inhibited - no growth ( upper right corner)

Front 13

Pseudomonas Isolation Agar

Back 13

designed for the selection of Pseudomonas species by the inclusion of the phenolic compond Triclosan.

Front 14

Pseudomonas species often produce ___________ pigments. Often ________ or __________ in color and sometimes pigments which fluoresce under ultraviolet light.

Back 14

Diffusible

Green or blue-green

Front 15

Pseudomonas Isolation Agar

Back 15

P.fluorescens ( upper left corner)

P aeruginosa ( upper right corner)

E. coli , no growth ( lower )

Front 16

PEA means

Back 16

Phenylethyl Alcohol agar

Front 17

PEA contains the inhibitory substance called?

Back 17

Phenylethyl alcohol

Front 18

PEA is a ____________ media.

Back 18

Selective in that is grows slow or prevents the growth of gram negative bacteria while allowing Gram positive organisms to grow.

Front 19

Example of Gram negative bacteria

Back 19

E. aerogenes

Front 20

Example of gram positive organism

Back 20

B. subtilis and E. faecalis

Front 21

Phenylethyl Alcohol agar

Back 21

Bacillus subtilis ( upper left corner)

Enterococcus faecalis ( upper right corner)

Enterobacter aerogenes ( lower )

Front 22

A unique characteristic of the PEA agar is?

Back 22

It gives off a rose odor caused by the substance phenylethyl.

Front 23

DNAse Agar

Back 23

Weak + (upper left corner)

Strong + ( upper right corner)

negative (lower)

Front 24

DNAse contains?

Back 24

Free DNA in the media

Front 25

Which enzyme, released by some organisms, breaks down the DNA into free nucleotides which can be taken up by the cell?

Back 25

Exonuclease

Front 26

The indicator in DNAse is?

Back 26

Methyl Green

Front 27

How can you tell if an organism has used some of the DNA in the DNAse agar?

Back 27

There will be a zone of clearing around the areas of growth because methyl green is ONLY green when bound to DNA.

Front 28

Blood Agar

Back 28

alpha (partial ) hemolysis (upper left)

Gamma (no) hemolysis (upper right)

beta (complete) hemolysis ( lower)

Front 29

Blood agar is a selective or differential media?

Back 29

differential which seperates organisms based on a hemolytic ability.

Front 30

Organisms that completely lyse red blood cells are?

Back 30

B-hemolytic

Front 31

B-hemolytic leave a ________ ________ around a colony.

Back 31

clear zone

Front 32

a-hemolytic organisms ___________ ___________ red blood cells.

Back 32

partially lyse

Front 33

In Blood agar a greenish zone around the colonies is caused by?

Back 33

partially lysed red blood cells

Front 34

Organisms showing no hemolysis are sometimes called?

Back 34

y-hemolytic

Front 35

Skim Milk Agar

Back 35

If the casein is broken down into amino acids (peptonization) a clear zone will be visable around the areas of growth.

Upper half is negative.

Lower half is positive (note the clearing area)

Front 36

What does Casein hydrolysis agar plates test for?

Back 36

It tests for the organisms's ability to release an exoenzyme ( casease) which breaks down the milk protein (casein) in the skim milk plates.

Front 37

Skim milk agar tests for the ability of an organism to release the exoenzyme

Back 37

casease

Front 38

The three primary physical parameters for describing a colony are:

Back 38

shape (configuration), margin and elevation

Front 39

MPN analysis is performed using tubes of _____ broth.

Back 39

lactose

Front 40

Kirby-Bauer (antibiotic disk-diffusion assays) are carried out using _____ plates

Back 40

Mueller-Hinton agar

Front 41

A positive MR test indicates the following end product(s):

Back 41

mixed acids

Front 42

MR-VP broth performs two tests that relate to the breakdown of:

Back 42

glucose

Front 43

What would you expect to see if you streaked a culture of Enterobacter aerogenes (a gram-negative organism) on phenylethyl alcohol agar?

Back 43

no growth

Front 44

Identify the organism growing on this Petri plate that is positive for starch hydrolysis

Back 44

A

Front 45

Bubbling of hydrogen peroxide placed on colony growth is a sign of the presence of the enzyme catalase. This is due to the production of:

Back 45

oxygen gas

Front 46

A positive starch test can be confirmed after adding Lugol's reagent. It is indicated by:

Back 46

a zone of clearing that is colorless to golden

Front 47

DNAse agar contains free DNA which can be broken down by the exoenzyme:

Back 47

exonuclease

Front 48

The reagent used in the catalase test is

Back 48

hydrogen peroxide

Front 49

The main substrate available for hydrolysis in skim milk agar is:

Back 49

casein

Front 50

The pH indicator found in Mannitol Salt agar is:

Back 50

phenol red

Front 51

The selective agent(s) in Eosin methylene Blue agar is/are:

Back 51

eosin Y and methylene blue

Front 52

Blood agar is able to distinguish between different types of organsims based on ____ ability?

Back 52

hemolysis

Front 53

The selective agent in Pseudomonas Isolation agar is:

Back 53

triclosan

Front 54

Looking at this Petri plate of organisms growing on Pseudomonas Isolation agar what can you conclude about Organism C?

Back 54

It is not a Pseudomonas

Front 55

Which of the following organisms plated onto the phenylethyl alcohol agar plate shown above is most likely Gram-positive

Back 55

Organism A and Organism B

Front 56

The potential end products that can be readily detected by observation of sugar (carbohydrate} broth tubes is/are:

Back 56

acid and gas

Front 57

The indicator present in Simmon's citrate media is:

Back 57

bromthymol blue

Front 58

The pH indicator present in a phenylalanine deaminase slant is:

Back 58

there is no pH indicator in this media

Front 59

. Which test is not determined by use of SIM media?

Back 59

mixed acids from glucose

Front 60

Which of the following tubes show a positive test for urease activity in urea broth?

Back 60

Tube B

Front 61

The reagent used to perform the MR test is:

Back 61

methyl red

Front 62

MR-VP broth performs two tests that relate to the breakdown of:

Back 62

glucose

Front 63

The MPN value for the sample shown above is:

Back 63

15

Front 64

Which of the following circles shown above represents a positive reaction for Stapylococcus aureus in the Staphyloslide test?

Back 64

#2

Front 65

In a positive oxidase test a _____ color should be seen within 30 seconds of applying bacteria to oxidase paper.

Back 65

dark blue or violet

Front 66

The oxidase test checks for the presence of cytochrome c oxidase an enzyme found in certain ______ bacteria.

Back 66

aerobic

Front 67

Before a stach hydrolysis test can be interpreted the following reagent must be added to the Petri plate:

Back 67

Lugol's

Front 68

DNAse agar contains free DNA which can be broken down by the exoenzyme

Back 68

exonuclease

Front 69

The chemical reaction catalysed by catalase is:

Back 69

hydrogen peroxide --> water to oxygen

Front 70

Organisms that produce clear halos around colony growth on Blood agar plates are said to be _____ hemolytic.

Back 70

beta

Front 71

The enzyme activity responsible for the visible reaction seen in this SIM tube is:

Back 71

cysteine desulfurase

Front 72

Pseudomonas Isolation agar is:

Back 72

selective

Front 73

What would you expect to see if you streaked a culture of Enterobacter aerogenes (a gram-negative organism) on phenylethyl alcohol agar?

Back 73

no growth

Front 74

The end product that is detected by the added reagents in a VP test is

Back 74

acetoin

Front 75

Which of the tubes pictured shows a negative VP test?

Back 75

Tube A

Front 76

Look at this nitrate broth. No gas was seen in the Durham tube. Nitrate A and Nitrate B have been added. What was the fate of the nitrate?

Back 76

reduced to nitrite

Front 77

Given the following information: The disk shown is for the antibiotic Ampicillin; the organism on the plate is Escherichia coli; The organism is most likely ____ to the antibiotic.

Back 77

resistant

Front 78

DNAse agar contains the indicator reagent ____ which allows for detection of the breakdown of DNA by microorganisms.

Back 78

Methyl green

Front 79

The reagent used in the catalase test is:

Back 79

hydrogen peroxide

Front 80

Phenylethyl alcohol agar is used to:

Back 80

select against Gram-negative organisms

Front 81

The potential end products that can be readily detected by observation of sugar (carbohydrate} broth tubes is/are:

Back 81

acid and gas

Front 82

Which of the following organisms placed on Simmon's citrate slants is positive for citrate utilization?

Back 82

Organism A

Front 83

Which of the phenylalanine tubes (above) show breakdown of phenylalanine to Phenylpyruvic acid and ammonia?

Back 83

Tube B

Front 84

Looking at the two SIM tubes shown here you can clearly determine that the organism is both:

Back 84

Indole+, sulfide+

Front 85

The type of aggltination test used in the lab where an antibody is attached to a latex particle to enhance visibility of the reaction is known as ___ agglutination.

Back 85

indirect

Front 86

What would be the logical explanation if tube B were clearly pink compared to tube A?

Back 86

ammonia was produced from amino acids

Front 87

Tube A shows a tube after nitrate reagents and zinc powder were added. The conclusion would be:

Back 87

nitrate was reduced to ammonia

Front 88

. The source of information used to create the dichotomous keys in the lab manual and for the species tables kept in the lab drawets is:

Back 88

Bergey's Manual

Front 89

The pH in a positive Simmon's citrate test is:

Back 89

basic

Front 90

Phenylalanine is broken down to form _____ and ammonia which can be detected on a pheylalanine slant using the reagent ferric chloride.

Back 90

phenylpyruvic acid

Front 91

Which of the following SIM tubes shows a non-motile organism?

Back 91

Tube A