Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
20 Cards in this Set
- Front
- Back
|
The most complex gland of the endocrine system is the: a. pancreas b. thyroid c. parathyroid d. pituitary |
THE CORRECT ANSWER IS "D." The pituitary is considered to be the most complex of all glands in the endocrine system. |
|
|
The preferred fixative for pituitary tissue sections prior to staining with Gomori's chrome-alum-hematoxylin is: a. Zenker's b. Helly's c. Bouin's d. Carnoy's |
THE CORRECT ANSWER IS "C." Picric acid in Bouin's fluid gives this tissue a strong affinity for acid dyes |
|
|
The combined Gomori methods for demonstrating pancreatic alpha and beta cells involves the use of these dyes: a. Ponceau-orange G b. aldehyde fuchsin-phloxine B c. congo red-hematoxylin d. chromotrope 2R-fast green |
THE CORRECT ANSWER IS "B." Dyes used in Gomori's combined method for pancreas are aldehyde fuchsin and phloxine B. |
|
|
A brown substance found in cells of the adrenal medulla, that is argentaffin in nature, is called: a. melanin b. lipofuscin c. chromaffin d. chromatin |
THE CORRECT ANSWER IS "C." The cytoplasm of cells of the adrenal medulla is filled with chromaffin granules that have the property of coloring brown when fixed with dichromate fixatives. |
|
|
Cells that are capable of being impregnated with silver by the use of a reducing agent are said to be: a. amphoteric b. argentaffin c. Argyrophilic d. autofluorescent |
THE CORRECT ANSWER IS "C." Argyrophilic cells can be impregnated with silver if a reducing agent, either chemical or light, is used to reduce impregnated silver to a metallic end product. |
|
|
One of the most commonly used staining procedures for demonstrating fibrin, that results in blue fibrin, red collagen, and blue nuclei is: a. Gomori's aldehyde fuchsin b. Mallory's phosphotungstic acid hematoxylin c. Carstair's method for fibrin d. Weigert's stain for fibrin |
THE CORRECT ANSWER IS "B." Although there are numerous fibrin stains, the FT AH is the only one with the stated results that is commonly used for this purpose. |
|
|
When cells can reduce silver to its visible metallic state without the aid of extraneous agents it is said to be a/an: a. argentaffin reaction b. decidual reaction c. argyrophilic reaction d. luteinizing reaction |
THE CORRECT ANSWER IS "A." A positive argentaffin reaction occurs when cells take up silver and reduce it to its visible metallic state without the use of a reducing agent. |
|
|
In addition to its ability to stain argentaffin cell granules, the Fontana-Masson technique may also be used to stain: a. lipids b. collagen c. melanin d. spirochetes |
THE CORRECT ANSWER IS "C." Melanin is argentaphilic and although this procedure is not specific for melanin, this pigment does have the ability to reduce silver nitrate to metallic silver. |
|
|
Granules of Paneth cells, found in the crypts of Lieberkühn of the small intestine, are intensely acidophilic and stain with: a. Harris' hematoxylin b. silver nitrate c. methylene blue d. orange G |
THE CORRECT ANSWER IS "D." Paneth cell granules are very acidophilic and stain with dyes such as orange G and eosin. |
|
|
Cells that are numerous in connective tissue and have cytoplasmic granules that stain metachromatically are: a. Purkinje cells b. macrophages c. argentaffin d. mast cells |
THE CORRECT ANSWER IS "D." Mast cells are widely distributed in connective tissue. Their cytoplasmic granules stain with metacnromatic dyes, but may also be stained with polychrome Giemsa, and methylene blue. |
|
|
One of the most meaningful, yet simple, techniques for identifying cell types in the pituitary gland is the: a. H&E b. Giemsa c. periodic acid-Schiff d. oil red O |
THE CORRECT ANSWER IS "C." Because of their glycoprotein content, PAS-positive granules of pituitary basophils can be distinguished from alpha cells. |
|
|
The oxidizing agent used in Gomori's chrome alum-hematoxylin-phloxine procedure for demonstrating alpha and beta cells of the pancreas is: a. periodic acid b. phosphotungstic acid c. potassium permanganate d. chromic acid |
THE CORRECT ANSWER IS "C." Potassium permanganate is a necessary oxidizing agent in this procedure when beta cells are to be demonstrated. |
|
|
Fixation in a primary chromate fixative is essential if chromaffin substance is to be demonstrated by this reduction method: a. modified Steiner b. Gomori iron c. trichrome-new fuchsin d. Schmorl's |
THE CORRECT ANSWER IS "D." Sections must be fixed in one of the chromate fixatives, Orth's or Moller's, to demonstrate chromaffin granules, as this reaction will not take place when tissues are exposed to formalin. |
|
|
Argentaffin cells found in the epithelium of the stomach and intestines are known as: a. Paneth cells b. enterochromaffin cells c. Bergmann's cells d. amphophilic cells |
THE CORRECT ANSWER IS "B." Argentaffin, or enterochromaffin cells, also called Kulchitsky cells, contain granules that stain readily with silver salts. |
|
|
A procedure that demonstrates carcinoid tumors as well as alpha cells of the pancreas is the: a. Sevier-Munger b. Warthin-Starry c. Grimelius d. periodic acid-Schiff |
THE CORRECT ANSWER IS "C." Although the Grimelius technique readily demonstrates these cells, the Sevier-Munger, also an argyrophilic procedure, demonstrates them very poorly, if at all. |
|
|
Oxytocin and antidiuretic hormone are two of the hormones secreted by this gland: a. pancreas b. thyroid c. pineal body d. pituitary |
THE CORRECT ANSWER IS "D." Oxytocin and antidiuretic hormones are produced by the posterior portion of the pituitary gland. |
|
|
Beta cells of pancreatic islets and of the pituitary gland stain deep purple with this procedure: a. Lillie azure A-eosin B b. congo red c. aldehyde fuchsin d. Gomori-Burtner |
THE CORRECT ANSWER IS "C." A fresh solution of aldehyde fuchsin stains beta cells deep purple in both the pancreas and pituitary glands. |
|
|
When using the combined Gomori method for pancreatic alpha and beta cells, the reagent used to differentiate phloxine so that alpha and beta cells are more clearly demonstrated, is: a. phosphomolybdic acid b. chromic acid c. phosphotungstic acid d. sodium thiosulfate |
THE CORRECT ANSWER IS "C." Differentiating in phosphotungstic acid removes the vivid red of phloxine stain from connective tissue so that alpha and beta cells can be distinguished. |
|
|
Chromaffin cell tumors of the adrenal gland are known as: a. adenomyomas b. carcinoids c. sarcomas d. pheochromocytomas |
THE CORRECT ANSWER IS "D." Pheochromocytomas arise in the chromaffin tissue portion of the adrenal medulla. |
|
|
A procedure for demonstrating cytoplasmic secretory granules of the juxtaglomerular complex is: a. Bowie's b. Von Kossa c. Mayer's mucicarmine d. Verhoeff-Van Gieson |
THE CORRECT ANSWER IS "A." Bowie's stain is an excellent method for demonstrating these cells, but requires fixation in Helly's fluid and overnight exposure of the sections to Bowie's solution. |
