Use LEFT and RIGHT arrow keys to navigate between flashcards;
Use UP and DOWN arrow keys to flip the card;
H to show hint;
A reads text to speech;
10 Cards in this Set
- Front
- Back
what is hematopoietic transplantation?
|
bone marrow transplant
|
|
What is Seed threshold?
* |
you have to get enough of the stem cells in the body for them to be stable and proliferate
|
|
how is cell culture expansion a challenge to stem cell use?
|
when you are replicated stem cells to get enough to use, they start to differentiate, making them no longer useful
|
|
What is adult stem cell therapy?
* |
take undifferentiated (these are still more differentiated than embryonic stem cells)
MESENCHYMAL CELLS out of your body |
|
What are the 2 forms of gene therapy?
|
Somatic transformation: deliver a wild type gene to somatic cells of an adult or child, without eliminating the mutant allele
Germ Line Transformation: replace a mutant gene with a wild type allele in the germ line (microinjection)--USED FOR RESEARCH ONLY |
|
what is ex vivo gene therapy
* |
take cells out
modify them inject them back in |
|
indirect gene therapies are used for what? examples?
|
to treat cancer
immune response: antigenic genes, transformed cells cleared Suicide genes: gene expression kills transformed cells Tumor suppressors: genes that inhibit tumorgenesis (p53) |
|
Challenges of gene therapy?
(objective-just read) |
Transformation level
Expression level Regulation Targeting to specific organs Transformation Stability |
|
Major danger in gene therapy?
|
Immunologic reaction--can lead to death
|
|
in ADA deficient SCIDs, what was the key to making gene therapy work?
|
they did ex vivo implantation
but the KEY IS TO KILL PRE-EXISTING T LYMPHOCYTES so that the new ones have room to grow in X linked form, the pts developed activation of oncogenes (womp womp) |