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45 Cards in this Set

  • Front
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Gold top-
additive
mode of action
uses
Additive: None

Mode of Action: Serum separator tube (SST) contains a gel at the bottom to separate blood from serum on centrifugation

Uses: Chemistries, Immunology and Serology
Light Green Top
Additive
mode of action
uses
Additive: Plasma Separating Tube (PST) with Lithium Heparin

Mode of Action: Anticoagulates with lithium heparin; plasma is separated with PST get at the bottom of the tube.

Uses: Chemistries
Purple Top
Additive
mode of action
uses
Additive: EDTA

Mode of Action: Forms calcium salts to remove calcium

Uses: Hematology (CBC) and Blood Bank (Crossmatch); requires FULL DRAW-invert 8 times to prevent clotting and platelet clumping.
Light Blue Top
Additive
mode of action
uses
additive: Sodium Citrate

Mode of action: Forms calcium salts to remove calcium

Uses: Coagulation tests (protime and prothrombin time), FULL DRAW required.
Green Top
Additive
mode of action
uses
Additive: Sodium heparin or lithium heparin

Mode of Action: Inactivates thrombin and thromboplastin

Uses: For lithium level, use sodium heparin. For ammonia level, use sodium or lithium heparin.
Dark Blue Top
Additive
Mode of Action
Uses
Additive: Sodium EDTA

Mode of Action: Tube is designed to contain no contaminating metals.

Uses: Trace element testing (zinc, copper, lead, mercury) and toxicology.
Light Gray Top
Additive
Mode of Action
Uses
Additive: Sodium flouride and potassium oxalate

Mode of Action: Antiglycolytic agent preserves glucose up to 5 days

Uses: For lithium level, use sodium heparin. Glucoses require FULL DRAW (may cause hemolysis if short draw)
Yellow Top
Additive
Mode of Action
Uses
Additive: ACD (acid-citrate-dextrose)

Mode of action: Complement inactivation.

Uses: HLA tissue typing, paternity testing, DNA studies
Yellow-Black Top
Additive
Mode of Action
Uses
Additive: Broth mixture

Mode of action: Preserves viability of microorganisms.

Uses: Microbiology-aerobes, aneaerobes, fungi
Black Top
Additive
Mode of Action
Uses
Additive: Sodium citrate (buffered)

Mode of action: Forms calcium salts to remove calcium

Uses: Westergren Sedimentation Rate; requires FULL DRAW
Orange Top
Additive
Mode of Action
uses
Additive: Thrombin

Mode of Action: Quickly clots blood

Uses: STAT serum chemistries
Light Brown Top
Additive
Mode of Action
uses
Additive Sodium heparin

Mode of Action: Inactivates thrombin and thromboplastin; Contains virtually no lead.

Uses: Serum Lead determination
Pink Top
Additive
Mode of Action
uses
Additive: Potassium EDTA

Mode of Action: Forms calcium salts

Uses: Molecular/viral load testing
red top tube is used for
Chemistries, immunology and serology, blood bank (crossmatch)
The gold top tube is used for
Chemistries, immunology and serology
The light green top tube is used for:
Chemistries
The red-gray top tube is used for
Chemistries
The purple top tube is used for
Hematology (CBC) and blood bank (crossmatch); requires FULL DRAW - invert 8 times to prevent clotting and platelet clumping
The light blue top tube is used for:
Coagulation tests (protime and prothrombin time), FULL DRAW required
The dark green top tube is used for
Lithium levels (use sodium heparin); ammonia levels (use sodium or lithium heparin)
The dark blue top tube is used for
Trace element testing (zinc, copper, lead, mercury) and toxicology
The light gray top tube is used for
Lithium levels (use sodium heparin); glucoses, requires FULL DRAW (may cause hemolysis if short draw)
The yellow top tube is used for
HLA tissue typeing, paternity testing, DNA studies
The yellow-black top tube is used for
Microbiology - aerobes, anaerobes, fungi
The black top tube is used for
Westergren sedimentation rate; requires FULL DRAW
The orange top tube is used for
STAT serum chemistries
The brown top tube is used for:
Serum lead determination
Centrifugation?
An apparatus consisting essentially of a compartment spun about a central axis to separate contained materials of different specific gravities, or to separate colloidal particles suspended in a liquid.
Immunology?
The branch of biomedicine concerned with the structure and function of the immune system, innate and acquired immunity, the bodily distinction of self from nonself, and laboratory techniques involving the interaction of antigens with specific antibodies.
Serology ?
The science that deals with the properties and reactions of serums, especially blood serum.
The characteristics of a disease or organism shown by study of blood serums: the serology of acquired immune deficiency syndrome; the serology of mammals.
Heparin ?
Heparin is an anticoagulant commonly used in chemistry and special chemistry testing. It is the recommended anticoagulant for many determinations using whole blood or plasma specimens because of its minimal chelating properties, minimal effects on water shifts, and relatively low cation concentration.
Lithium Heparin
There are currently three salts of heparin that are commonly used in blood collection devices: ammonium, lithium and sodium. Lithium heparin is the recommended form of heparin to be used because it is least likely to interfere when performing tests for other ions. Lithium heparin is essentially free of extraneous ions. It should not be used for collection of blood for lithium levels.
Anticoagulant
A substance that prevents the clotting of blood
Plasma
The clear, yellowish fluid portion of blood, lymph, or intramuscular fluid in which cells are suspended. It differs from serum in that it contains fibrin and other soluble clotting elements
Calcium Citrate
calcium salt of citric acid. Calcium citrate is also used as a water softener because the citrate ions can chelate unwanted metal ions.
Anti Glycolytic Agent
keeps glucose from braking down
EDTA
Chemistry, Pharmacology. ethylenediaminetetraacetic acid: a colorless compound, C10H16N2O8, capable of chelating a variety of divalent metal cations: as a salt used as an anticoagulant, antioxidant, blood cholesterol reducer, food preservative; as a calcium-disodium salt used in the treatment of lead and other heavy-metal poisonings.
Viral Load Testing
The viral load test measures the amount of HIV virus in your blood.
Sedementaion Rate
The erythrocyte sedimentation rate (ESR), also called a sedimentation rate, sed rate or 'Biernacki Reaction', is a non-specific measure of inflammation that is commonly used as a medical screening test. The ESR is increased by any cause or focus of inflammation. The ESR is decreased in sickle cell anemia, polycythemia, and congestive heart failure. The basal ESR is slightly higher in females
HLA Tissue Typing
Tissue type is a blood test that measures substances called antigens that determine whether donor tissue is compatible for transplant into another person. This test may also be called HLA typing. Antigens on the surface of body cells and tissues can tell the difference between normal body tissue or foreign tissue (for example, tissue from another person's body). Tissue type helps identify the best donor for tissues or blood cells (such as platelets). In some cases, tissue typing may be done to determine a person's risk for developing certain diseases, especially certain autoimmune diseases.
Anti Glycolytic Agent
keeps glucose from braking down
EDTA
Chemistry, Pharmacology. ethylenediaminetetraacetic acid: a colorless compound, C10H16N2O8, capable of chelating a variety of divalent metal cations: as a salt used as an anticoagulant, antioxidant, blood cholesterol reducer, food preservative; as a calcium-disodium salt used in the treatment of lead and other heavy-metal poisonings.
Viral Load Testing
The viral load test measures the amount of HIV virus in your blood.
Sedementaion Rate
The erythrocyte sedimentation rate (ESR), also called a sedimentation rate, sed rate or 'Biernacki Reaction', is a non-specific measure of inflammation that is commonly used as a medical screening test. The ESR is increased by any cause or focus of inflammation. The ESR is decreased in sickle cell anemia, polycythemia, and congestive heart failure. The basal ESR is slightly higher in females
HLA Tissue Typing
Tissue type is a blood test that measures substances called antigens that determine whether donor tissue is compatible for transplant into another person. This test may also be called HLA typing. Antigens on the surface of body cells and tissues can tell the difference between normal body tissue or foreign tissue (for example, tissue from another person's body). Tissue type helps identify the best donor for tissues or blood cells (such as platelets). In some cases, tissue typing may be done to determine a person's risk for developing certain diseases, especially certain autoimmune diseases.