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9 Cards in this Set
- Front
- Back
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What is recombinant dna technology |
Combining different organisms dna, to manipulate and alter genes to improve industrial processes and medical treatments |
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Steps in recombinant dna technology for in vivo |
Is india transforming its curry Isolation Insertion Transformation Identification cloning |
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Methods to isolate dna fragments for recombinant DNA technology |
Reverse transcription Restriction endonuclease Gene machine |
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Method of PCR |
In thermocycler, temperature is increased to 95C to break h bonds and split dna into single strands Temperature decreased to 55C to allow primers to attach(anneal) DNA polymerase attach complementary free nucleotides to align Temperature increased to 72C for taq dna polymerase to make dsDNA |
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How do dna probes work with dna hybridisation |
Sample of patient dna removed and heated to make it single stranded Mixed with dna probes that are complementary to allele with heritable condition If patient has allele for condition, their dna binds to the probe, and can be identified |
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Process of genetic fingerprinting |
Collection Extraction Digestion Separation Hybridisation Development Analysis |
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How does restriction endonuclease work |
- Cut up dna at recognition sequence - blunt ends formed when cut occurs between two opposite pairs -Sticky ends formed when cut staggered, exposing dna bases- known as palindromic |
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What to do with dna before insertion |
-Promoter region added - at start of dna fragment- to ensure dna polymerase can attach for transcription to occur - terminator region- attend of gene- for rna polymerase to detach and stop transcription so only 1 gene is copied |
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How to identify transformation |
Marker genes: antibiotic resistance, fluorescent markers , enzyme markers |
