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16 Cards in this Set
- Front
- Back
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The use of chemicals to control infection: |
Antimicrobial chemotherapy |
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Microorganisms are becoming resistant to what? |
Antimicrobial agents |
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Something that will specifically target and inhibit or destroy a microbe without harming the host |
Magic bullet |
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Antimicrobial substances that are produced by a microorganism |
True antibiotic |
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Naturally produced molecules that have been chemically modified to make them more effective |
Semisynthetic antimicrobial agents |
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Purely manmade molecules |
Synthetic antimicrobial agents |
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Susceptibility pattern shows what? |
Whether the patient is resistant, susceptible, or has intermediate resistance |
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What test is a commonly used method of determining susceptibility |
Kirby Bauer method |
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Kirby Bauer is what type of test? |
Standardized disk diffusion test |
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In the kirby Bauer test, a petri dish containing what is poured to a uniform thickness of 4mm? |
Mueller Hinton agar |
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How do we read the results for disk diffusion test? |
We measure the zones of inhibition and record whether the organism was resistant, susceptible, or intermediate resistant |
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What is the cleared ring called in the disk diffusion test? |
Zone of inhibition |
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The ability of a bacteria to take up DNA fragments from the environment and ultimately express the information encoded in the genes of that DNA is termed: |
Transformation |
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Describe heat shock: |
Submerging 1/2 of the tube in 42 degree C water bath and agitating both of the tubes by gently shaking them back and forth for 90 seconds |
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Why do we heat shock? |
It allows the DNA to readily enter the cells |
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Why wouldn't we expect a lawn of bacteria on the LBA/AMP + plasmid? |
Because only some take up the plasmid and not all grow with it. That's why we would get # of colonies and not a lawn |
