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11 Cards in this Set
- Front
- Back
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Staphylococcus aureus
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o Media: BAP
o large (2-3 mm in diameter), opaque, creamy colonies o Narrow zone of β-hemolysis. O Gram-positive cocci in clusters. o Catalase (positive), Coagulase (positive), Mannitol / Salt (positive) |
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Staphylococcus epidermidis
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o Media: BAP
o large (2-3 mm in diameter), opaque, white colonies o No hemolysis o Gram-positive cocci in clusters. o Catalase (positive), Coagulase (negative), Sensitive to the drug Novobiocin |
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Streptococcus pyogenes (GAS)
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o Media: BAP
o very small(.5-1mm), grayish, translucent colonies o large zone of β-hemolysis around colony o Gram-positive cocci in chains. o Catalase (negative), Sensitive to the drug Bacitracin |
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Streptococcus agalactiae (GBS)
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o Media: BAP
o Medium size (~1.5 mm in diameter), grayish, translucent colonies o Narrow zone of β-hemolysis around colony, often times seen under colony after scraping it away o Gram-positive cocci in short chains. o Catalase test (negative), CAMP test (positive) *Resembles Listeria monocytogenes. |
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Streptococcus pneumoniae
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o Media: BAP
o Small(~1mm ), flat, mucoid (encapsulated) colonies o Prominent zone of α-hemolysis around colony o Gram-positive lancet-shaped diplococci o Catalase test (negative), Bile solubility test (positive), Sensitive to the drug Optochin |
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Enterococcus faecalis:
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Media: BAP
o Small to medium size (1-1.5 mm in diameter) grayish-white colonies o No hemolysis o Gram-positive cocci in short chain o Catalase (negative), Esculin (positive), NaCl test (positive) |
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Haemophilus influenzae
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o Media: chocolate (requires X (hemin) and H (NAD) factors for optimal growth)
o Medium (~2 mm in diameter), grayish mucoid colonies(dew-drop appearance) with a distinctive odor (musty basement) o No hemolysis o Gram-negative, pleomorphic (different shapes) coccobacilli o Oxidase (positive), Satellite test (Positive) |
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Neisseria meningitidis
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o Media: BAP & Choc
o Small (~1 mm in diameter), grayish, glistening, mucoid colonies o No hemolysis o Gram-negative, kidney-bean shaped diplococci. o Catalase (positive), Oxidase (positive), glucose (positive), maltose (positive), |
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Neisseria gonorrhoeae
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o Media: chocolate
o Small (~1 mm in diameter), grayish, glistening , mucoid colonies o No hemolysis o Gram-negative, kidney-bean shaped diplococci. o Catalase (positive), Oxidase (positive), glucose (positive), maltose (negative) |
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Corynebacterium diphtheriae
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o Media: BAP
o Small (~1 mm in diameter), white colonies with distinctive garlic odor o No Hemolysis o Gram-positive, straight rods (Chinese characters or X-Y arrangements) Catalase (positive) Toxin Demonstration (Elek Test) |
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Listeria monocytogenes
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o Media: BAP
o Small (~1 mm in diameter), white-grayish colonies o Small zone of β-hemolysis o Gram-positive, pleomorphic (resembles Corynebacterium & Streptococci) -at 25 C (RT) G+ rods, motile -at 37 C (body T) G+ cocci, nonmotile o Catalase (positive), Motility test at RT (positive), CAMP test (positive) *Resembles GBS |
