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12 Cards in this Set
- Front
- Back
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Recognition of Ori Site
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Prokaryote:
DNA A protein Eukaryote: unknown |
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Unwinding of double helix
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Prokaryote:
Helicase (req's ATP) Eukaryote: Helicase (req's ATP) |
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Stabilization of unwound strands
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Prokaryote:
SSB protein Eukaryote: SSB protein |
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Synthesis of RNA Primers
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Prokaryote:
primase Eukaryote: primase |
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Synthesis of DNA (Leading & Lagging strands)
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Prokaryote:
leading & lagging: DNA polymerase III Eukaryote: leading: DNA poly delta lagging: DNA poly alpha |
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Removal of RNA primers
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Prok:
DNA polymerase I (5'-->3' exonuclease) ...remember that the 3' exonuclease part of DNA poly III as a correction tool Euk: unknown |
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Replacement of RNA with DNA
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Prok:
DNA poly I Euk: unknown |
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Joining of Okazaki Fragments
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Prok:
DNA ligase I (req NAD) Euk: DNA ligase I (req ATP) |
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Removal of supercoils ahead of advancing replication forks
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Prk:
DNA topoisomerase II (DNA gyrase) [inh. by quinolones like nalidixic acid, ciprofloxacin, norlfoxacin] Euk: DNA topoisomerase II [inh by anticancer: etoposide, teniposide] |
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Synthesis of telomeres
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Prk: none
Euk: telomerase (adds to the end of the DNA b/c human DNA poly cuts off a bit with each replication) |
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DNA Damage:
Thymine Dimers (cause, recognition/excision enzyme, repair enzyme) |
cause: UV radiation
cell cycle phase: G1 recognition/excision enzyme: Excision endonuclease-->deficient in Xeroderma Pigmentosum repair enzyme: DNA poly, DNA ligase |
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DNA Damage:
Mismatched base (cause, recognition/excision enzyme, repair enzyme) |
case: DNA replication error
cell cycle phase: G2 recognition/excisioin enzyme: mutation of hMSH2 or hMLH1 gene (recognition & repair genes) leads to HNPCC (lynch syndrome) repair enzyme: DNA poly, DNA ligase |
