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19 Cards in this Set
- Front
- Back
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Mito e- chain: Complex I alt names
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NADH dehydrogenase
NADH -CoQ reducatase |
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Mito e- chain: Complex I Prosthetic Group
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FMN
Fe*S |
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Mito e- chain: Complex II alt names
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Succinate dehydrogenase
Succinate-CoQ reducatase |
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Mito e- chain: Complex II Prosthetic Group
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FAD
Fe*S |
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Mito e- chain: Complex III alt names
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Ub:cytochrome c Oxidoreductase
CoQH2-cytochrome c reductase |
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Mito e- chain: Complex III Prosthetic Group
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Heme
Fe*S |
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Mito e- chain: cytochrome c Prosthetic groups
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Heme
cytochrome c doesn't have alt name |
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Mito e- chain: Complex IV alt names
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cytochrome c oxidase
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Mito e- chain: Complex IV Prosthetic Group
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Heme
Cu-A, Cu-B |
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Y Y A N T/A Y Y
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Initiator (Inr
located -2 to +5 Recognized by TAF250 or TAF150 subunits of TFIID Exact sequence may help determine promoter strength • Some promoters have Inr instead of a TATA box - Some promoters have both Inr and a TATA box - Some promoters have neither |
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A/G G A/T C/T G T
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Downstream promoter element (DPE):
Located about 30 bp downstream of Inr Recognized by TAF60 and TAF40 subunits of TFIID • Best understood in Drosophila, may occur in vertebrates |
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assembly of Pre-initiation complex
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TFIID
B F w/pol II E H |
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What are the Core Promoter Elements?
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TATA
Initiator (Inr): Y Y A N T/A Y Y Downstream promoter element (DPE): A/G G A/T C/T G T |
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What are the Regulatory elements?
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1. CAAT box: G G C C A A T C T
2. GC box: G G G C G G 3. Octomer: A T T T G C A T 4. KappaB: G G G A C T T T C C |
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G G C C A A T C T
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CAAT box
about -80, but position can vary recognized by CTF family |
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G G G C G G
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GC box
- Recognized by SP1 |
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A T T T G C A T
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Octomer
- Sometimes present in more than one copy - Recoginized by Oct-1 and Oct-2 - animals |
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G G G A C T T T C C
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KappaB
Recognized by NFkB and H2-TF-1 |
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These are used to figure out if where important sites are for gene activity
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if you have TF:
1. methylation interference 2. DNase I footprinting This will give us binding, but need to know if it's Up/Down --> Then do linker scanning to get specific parts or can do Run off to get Promoter, but not enhancers |
