Patho 3

Front 1

Mange or scrub mites

Back 1

_________ may be licked or nibbled from the skin, thus accounting for their appearance in the feces.

Front 2

status of digestion

Back 2

Fecal examination may also reveal the _________ as is shown by the presence of starch, fat droplets, of undigested muscle

Front 3

pseudoparasites

Back 3

Animals may swallow certain objects that resemble parasite forms.

Front 4

pollen grains

plant hairs

grain mites

mold spores

harmless plant & animal debris

Back 4

Examples of pseudoparasites

Front 5

spurious

Back 5

parasite eggs or cyst from one species of host may be found in the feces of a scavenger or predator host as the result of coprophagy.

Front 6

dehydrated

Back 6

Old samples may become ________, making suspension difficult

Front 7

true

Back 7

worm ova or coccidial cyst may undergo development, hatching or disintegration to such a degree as to interfere with diagnosis

Front 8

10% formalin solution

Back 8

If fecal material is to be transported for more than a few hours it must be preserved using __________ to saturate the sample.

Front 9

Refrigeration

Back 9

will also keep samples in good condition for several days.

Front 10

10x

Back 10

The fixative/preservative should be _____ the volume of the specimen

Front 11

Microscopic Examination of Feces

Back 11

This may include several techniques such as the

Simple Fecal Smear Method

Qualitative Concentration Method

Quantitative Concentration Method

Front 12

Simple Fecal Smear Method

Back 12

This technique should be used only when very small samples are available or when lack of equipment or time prevents the use of more accurate techniques.

Front 13

Simple Fecal Smear

Back 13

what technique involves placing a drop of tap water on the center of the slide

Front 14

grain of rice

Back 14

In Simple Fecal Smear,

with a toothpick, or some similar instrument, detach from the fecal mass a small amount, about the size of a ______

Front 15

LOW POWER

Back 15

magnification in Simple Fecal Smear

Front 16

sodium chloride

sucrose

glycerine

zinc sulfate

zinc acetate

sodium nitrate sodium acetate

magnesium sulfate.

Back 16

heavy flotation solutions

Front 17

Glycerine

Back 17

has too high a viscosity, hence flotation is slow.

Front 18

saline solutions

Back 18

low in viscosity but they tend to dehydrate and thus distort parasite forms

Front 19

saline solutions

Back 19

also they crystallize rather quickly on the microslide.

Front 20

high specific gravity

Back 20

Solutions of _____ will float too much debris, thus defeating the purpose for which they are intended.

Front 21

Sugar Flotation Technique

Back 21

In Sugar Flotation Technique, emulsify ________ gram of feces in 3 ml sugar solution in a vial or other suitable container

Front 22

20 minutes

Back 22

In Sugar Flotation Technique, apply coverglass or slide to surface film for __________

Front 23

wet side

Back 23

Remove coverglass with adherent fluid and place, _______ down, on a slide for examination. If slide is used for surface films, mount with coverglass.

Front 24

Willi's Brine Flotation Technique

Back 24

This method is unsatisfactory for protozoan cysts and trematode ova.

Front 25

1 gram, 2ml

Back 25

In Willi's Brine Flotation Technique, emulsify ________ of feces in _____ ml of brine in a glass cylinder about 2.5 cm in diameter.

Front 26

steel wool

Back 26

In Willi's Brine Flotation Technique, he cylinder may be plugged with a small amount of _______ to hold down the large fecal particles.

Front 27

meniscus

Back 27

Add enough brine to form a _____ at the top of the tube

Front 28

10-60 minutes

Back 28

In Willi's Brine Flotation Technique, place a clean slide on the meniscus and allow it to remain for ________ minutes. Remove the slide, invert and examine.

Front 29

Modified Fluke Egg Technique

Back 29

Add 15 ml detergent solution then mix well with a stirring rod

Add 15 ml detergent solution then mix well with a stirring rod

Front 30

15 ml

Back 30

Modified Fluke Egg Technique, add ____ ml detergent solution then mix well with a stirring rod

Front 31

50 ml centrifuge

Back 31

In Modified Fluke Egg Technique, strain the mixture through the funnel strainer into a ________ tube

Front 32

flooding, swirling

Back 32

Pour enough detergent solution in a ______,______ motion through the feces in the funnel strainer to fill the centrifuge tube

Front 33

5 to 15 minutes

Back 33

In Modified Fluke Egg Technique, Allow the mixture to stand for ______

Front 34

3/4

Back 34

Decant ____ of the fluid from the centrifuge tube

Front 35

true

Back 35

Rewash the fecal materials in the funnel-strainer to refill the centrifuge tube, in order to obtain any ova trapped previously

Front 36

2-3 ml

Back 36

Decant all liquid down to about . Do not disturb the sediment

Front 37

Iodine

Back 37

Add 2-3 drops of ______ to the sediment, allowing the sediment to stand for 2-5 minutes

Front 38

Modified Fluke Egg Technique

Back 38

Using a pipette, transfer the sediment to a microslide and apply coverglass

Front 39

LPO

Back 39

Search the sediment on the slide using the _______ of microscope.

Front 40

McMaster Egg-Counting Technique

Back 40

This is placed in a glass jar containing beads or in a mortar of small basin

Front 41

3 grams

Back 41

In McMaster Egg-Counting Technique, the sample of feces is well mixed and _____ grams are weighed out.

Front 42

58 ml

Back 42

To it is added ______ml of saturated (or half-saturated) salt solution and are vigorously mixed until an even emulsion is obtained.

Front 43

McMaster Egg-Counting Technique

Back 43

The suspension may be passed through a fine mesh strainer (coffee strainer) to remove the larger debris if desired.

Front 44

40% sugar solution

33% saturated zinc sulfate solution

Back 44

what can be used instead of the saturated salt solution?

Front 45

wide bore Pasteur pipette

Back 45

While mixing is in progress a sample is quickly withdrawn with a ________pipette and the material run into the mounting chamber of the McMaster slide.

Front 46

McMaster Egg-Counting Technique

Back 46

The slide is then left for 1-2 minutes to allow the eggs to float to the surface of the salt or sugar solution

Front 47

McMaster Egg-Counting Technique

Back 47

total number of eggs appearing within the ruled areas of the uppermost slide is counted microscopically.

Front 48

McMaster Egg-Counting Technique

Back 48

The eggs rest on the under surface of the upper slide, and with a low-power magnification both the ruled areas and the eggs are in focus.

Front 49

0.15 cm2

Back 49

The number of eggs in each cm2represent the eggs in ______ of the emulsion.

Front 50

Total number of eggs in the one chamber multiplied by 200 or average number of eggs in the 2 chambers multiplied by 200

Back 50

e.p.g formula